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MiR-203 suppresses ZNF217 upregulation in colorectal cancer and its oncogenicity.

Li Z, Du L, Dong Z, Yang Y, Zhang X, Wang L, Li J, Zheng G, Qu A, Wang C - PLoS ONE (2015)

Bottom Line: In this study, we demonstrated that ZNF217 expression was aberrantly upregulated in CRC tissues and associated with poor overall survival of CRC patients.Furthermore, combined treatment of ZNF217 siRNA and miR-203 exhibited synergistic inhibitory effects.Taken together, our results provide new evidences that ZNF217 has an oncogenic role in CRC and is regulated by miR-203, and open up the possibility of ZNF217- and miR-203-targeted therapy for CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China.

ABSTRACT
Zinc finger protein 217 (ZNF217) is essential for cell proliferation and has been implicated in tumorigenesis. However, its expression and exact roles in colorectal cancer (CRC) remain unclear. In this study, we demonstrated that ZNF217 expression was aberrantly upregulated in CRC tissues and associated with poor overall survival of CRC patients. In addition, we found that ZNF217 was a putative target of microRNA (miR)-203 using bioinformatics analysis and confirmed that using luciferase reporter assay. Moreover, in vitro knockdown of ZNF217 or enforced expression of miR-203 attenuated CRC cell proliferation, invasion and migration. Furthermore, combined treatment of ZNF217 siRNA and miR-203 exhibited synergistic inhibitory effects. Taken together, our results provide new evidences that ZNF217 has an oncogenic role in CRC and is regulated by miR-203, and open up the possibility of ZNF217- and miR-203-targeted therapy for CRC.

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IHC staining of ZNF217 in CRC.(A-Left) Cytoplasma staining of ZNF217 in CRC cells. (A-Right) Lack of ZNF217 expression in normal colonic epithelial cells. (B) The percentage of positively stained cells in cancer and adjacent normal tissues (*P < 0.05, **< 0.01). (C) Kaplan–Meier analysis of overall survival in CRC patients according to ZNF217 expression level. The ZNF217 positive group (n = 55) showed significantly shorter survival compared with the negative group (n = 27; P = 0.0405: log-rank test).
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pone.0116170.g001: IHC staining of ZNF217 in CRC.(A-Left) Cytoplasma staining of ZNF217 in CRC cells. (A-Right) Lack of ZNF217 expression in normal colonic epithelial cells. (B) The percentage of positively stained cells in cancer and adjacent normal tissues (*P < 0.05, **< 0.01). (C) Kaplan–Meier analysis of overall survival in CRC patients according to ZNF217 expression level. The ZNF217 positive group (n = 55) showed significantly shorter survival compared with the negative group (n = 27; P = 0.0405: log-rank test).

Mentions: IHC analysis of 82 cases of CRC and their corresponding noncancerous tissues showed that positive staining for ZNF217 was seen in the cytoplasma of CRC cells and corresponding non-cancerous mucosa cells (Fig. 1A). The mean percentages of cells stained positive for ZNF217 in cancerous and corresponding non-cancerous mucosa were 76.3% and 38.9%, respectively. By comparing the percentage of positive cells, it was determined that ZNF217 expression in colorectal carcinoma was statistically higher than that in the adjacent non-cancerous mucosa (P < 0.001; Fig. 1B).


MiR-203 suppresses ZNF217 upregulation in colorectal cancer and its oncogenicity.

Li Z, Du L, Dong Z, Yang Y, Zhang X, Wang L, Li J, Zheng G, Qu A, Wang C - PLoS ONE (2015)

IHC staining of ZNF217 in CRC.(A-Left) Cytoplasma staining of ZNF217 in CRC cells. (A-Right) Lack of ZNF217 expression in normal colonic epithelial cells. (B) The percentage of positively stained cells in cancer and adjacent normal tissues (*P < 0.05, **< 0.01). (C) Kaplan–Meier analysis of overall survival in CRC patients according to ZNF217 expression level. The ZNF217 positive group (n = 55) showed significantly shorter survival compared with the negative group (n = 27; P = 0.0405: log-rank test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306553&req=5

pone.0116170.g001: IHC staining of ZNF217 in CRC.(A-Left) Cytoplasma staining of ZNF217 in CRC cells. (A-Right) Lack of ZNF217 expression in normal colonic epithelial cells. (B) The percentage of positively stained cells in cancer and adjacent normal tissues (*P < 0.05, **< 0.01). (C) Kaplan–Meier analysis of overall survival in CRC patients according to ZNF217 expression level. The ZNF217 positive group (n = 55) showed significantly shorter survival compared with the negative group (n = 27; P = 0.0405: log-rank test).
Mentions: IHC analysis of 82 cases of CRC and their corresponding noncancerous tissues showed that positive staining for ZNF217 was seen in the cytoplasma of CRC cells and corresponding non-cancerous mucosa cells (Fig. 1A). The mean percentages of cells stained positive for ZNF217 in cancerous and corresponding non-cancerous mucosa were 76.3% and 38.9%, respectively. By comparing the percentage of positive cells, it was determined that ZNF217 expression in colorectal carcinoma was statistically higher than that in the adjacent non-cancerous mucosa (P < 0.001; Fig. 1B).

Bottom Line: In this study, we demonstrated that ZNF217 expression was aberrantly upregulated in CRC tissues and associated with poor overall survival of CRC patients.Furthermore, combined treatment of ZNF217 siRNA and miR-203 exhibited synergistic inhibitory effects.Taken together, our results provide new evidences that ZNF217 has an oncogenic role in CRC and is regulated by miR-203, and open up the possibility of ZNF217- and miR-203-targeted therapy for CRC.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong Province, China.

ABSTRACT
Zinc finger protein 217 (ZNF217) is essential for cell proliferation and has been implicated in tumorigenesis. However, its expression and exact roles in colorectal cancer (CRC) remain unclear. In this study, we demonstrated that ZNF217 expression was aberrantly upregulated in CRC tissues and associated with poor overall survival of CRC patients. In addition, we found that ZNF217 was a putative target of microRNA (miR)-203 using bioinformatics analysis and confirmed that using luciferase reporter assay. Moreover, in vitro knockdown of ZNF217 or enforced expression of miR-203 attenuated CRC cell proliferation, invasion and migration. Furthermore, combined treatment of ZNF217 siRNA and miR-203 exhibited synergistic inhibitory effects. Taken together, our results provide new evidences that ZNF217 has an oncogenic role in CRC and is regulated by miR-203, and open up the possibility of ZNF217- and miR-203-targeted therapy for CRC.

Show MeSH
Related in: MedlinePlus