Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells.
Bottom Line: Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression.In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS).Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.
Affiliation: Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, United Kingdom.Show MeSH
Mentions: To begin to characterise eRNA expression, we initially examined the time course of LPS-induced expression of two key inflammatory genes, SOD2 and IL6[17–19] and the six immune-related eRNAs, SLC30A4-eRNA, SOC3-eRNA, AZIN1-eRNA, TNFSF8-eRNA, MARCKS-eRNA and ACSL1-eRNA. As expected, we observed a rapid induction of SOD2 that peaked at 6 h and a steady increase in IL6 expression throughout the 24 h period (Fig. 2a). All of the eRNAs demonstrated rapid induction with expression peaking between 2 h and 6 h (Fig. 2b).
Affiliation: Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, United Kingdom.