Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells.
Bottom Line: The effects of acetylcholine on axonal information processing, though, remain unknown.In support, immunohistochemistry revealed muscarinic M1 receptor, CaV3.2, and KV7.2/7.3 subunit localization in granule cell axons.Since alterations in axonal signaling affect neuronal firing patterns and neurotransmitter release, this is an unreported cellular mechanism by which acetylcholine might, at least partly, enhance cognitive processing.
Affiliation: UCL School of Pharmacy, University College London, London, WC1N 1AX, UK.Show MeSH
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Mentions: We next asked whether T-type Ca2+ channels are present in mossy fibers. Three subunits, CaV3.1–3.3, encode for T-type Ca2+ channels (Catterall, 2011). CaV3.2 antibody immunogold particles were detected on wild-type axons, dendrites, and somata spines (Figure 7A). The granule cell axon CaV3.2 labeling, although less than in dendrites and spines, was significantly greater than that in CaV3.2 granule cell axons (Figure 7A), indicating that CaV3.2 subunits are located in mossy fibers.
Affiliation: UCL School of Pharmacy, University College London, London, WC1N 1AX, UK.