Cholinergic afferent stimulation induces axonal function plasticity in adult hippocampal granule cells.
Bottom Line: The effects of acetylcholine on axonal information processing, though, remain unknown.In support, immunohistochemistry revealed muscarinic M1 receptor, CaV3.2, and KV7.2/7.3 subunit localization in granule cell axons.Since alterations in axonal signaling affect neuronal firing patterns and neurotransmitter release, this is an unreported cellular mechanism by which acetylcholine might, at least partly, enhance cognitive processing.
Affiliation: UCL School of Pharmacy, University College London, London, WC1N 1AX, UK.Show MeSH
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Mentions: To investigate how endogenous acetylcholine affects granule cell intrinsic activity, we made patch-clamp recordings from mature cells in brain slices before and after extracellular stimulation of afferents in the stratum moleculare (Figure 1A). These cells had input resistances (RN) of 297.94 ± 23.9 MΩ (n = 117) and complex dendritic trees (Figure 1A) as revealed by post hoc morphological analysis. Experiments were performed in the presence of glutamatergic and GABAergic ionotropic and metabotropic receptor inhibitors, unless otherwise stated.
Affiliation: UCL School of Pharmacy, University College London, London, WC1N 1AX, UK.