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Diversity of cortical interneurons in primates: the role of the dorsal proliferative niche.

Radonjić NV, Ayoub AE, Memi F, Yu X, Maroof A, Jakovcevski I, Anderson SA, Rakic P, Zecevic N - Cell Rep (2014)

Bottom Line: In human and nonhuman primates, evidence suggests that an additional subset of neocortical GABAergic interneurons is generated in the cortical VZ and a proliferative niche, the outer SVZ.The origin, magnitude, and significance of this species-specific difference are not known.Our findings suggest that these progenitors constitute an evolutionary novelty contributing to the elaboration of higher cognitive functions in primates.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030, USA; Institute of Medical and Clinical Biochemistry, School of Medicine, University of Belgrade, Pasterova 2, 11000 Belgrade, Serbia.

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At P1, Cells Electroporated with hNkx2.1 at E14.5 Express Lhx6 and GABA(A) The overlay image (panel on the left) and separate channels (optical sectioning panels) are shown on the right. The cell in the center is transfected with EGFP+ (yellow) and colabeled with Nkx2.1 (green) and Lhx6 (red) in the VZ/SVZ.(B) Transfected EGFP+ cells (green) are colabeled (arrowheads) with GABA (red). The overlay image (panel on the left) and separate channels (optical sectioning) are shown on the right. Insets: drawings of sections with boxed areas presented on photographs.Scale bars represent 50 μm (A) and 20 μm (B).
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Figure 6: At P1, Cells Electroporated with hNkx2.1 at E14.5 Express Lhx6 and GABA(A) The overlay image (panel on the left) and separate channels (optical sectioning panels) are shown on the right. The cell in the center is transfected with EGFP+ (yellow) and colabeled with Nkx2.1 (green) and Lhx6 (red) in the VZ/SVZ.(B) Transfected EGFP+ cells (green) are colabeled (arrowheads) with GABA (red). The overlay image (panel on the left) and separate channels (optical sectioning) are shown on the right. Insets: drawings of sections with boxed areas presented on photographs.Scale bars represent 50 μm (A) and 20 μm (B).

Mentions: At P1, the pattern of Nkx2.1 staining in the mouse control brain (Figure S5) was similar to that previously described (Marin et al., 2000). Nkx2.1 expression was restricted to the basal ganglia, while in the cortex only sparse (isolated) Nkx2.1+ cells could be identified, similar to previous reports in neonatal mice (Ohira et al., 2010). In control animals electroporated with (pCAGG-EGFP), transfected cells were not colabeled with the anti-Nkx2.1 antibody and were predominantly located in the upper cortical layers II/III of the cortex and less often in the VZ/SVZ (Figure S5B). In contrast, hNkx2.1/EGFP+ transfected cells were mainly located in the VZ, although individual cells with migratory, bipolar morphology were also demonstrated throughout the cerebral wall, from the ventricular to pial surface (Figure S5C). Immunohistochemical analyses showed that the majority of electroporated (hNkx2.1/EGFP+) cells were colabeled with the antibody to Nkx2.1 (Figure S5C) or Lhx6+ and were distributed in the VZ/SVZ, but rarely in the cortical plate (Figure 6A). In addition, a number of electroporated cells expressed GABA (Figure 6B) and were occasionally labeled with CalR, whereas other interneuron markers, such as PV or Sst, did not colabel cortical cells at P1. This finding indicates that after 7 days of differentiation, ectopic expression of hNkx2.1 gene is sufficient to impart GABAergic-like properties in neurons generated in the mouse dorsal pallium.


Diversity of cortical interneurons in primates: the role of the dorsal proliferative niche.

Radonjić NV, Ayoub AE, Memi F, Yu X, Maroof A, Jakovcevski I, Anderson SA, Rakic P, Zecevic N - Cell Rep (2014)

At P1, Cells Electroporated with hNkx2.1 at E14.5 Express Lhx6 and GABA(A) The overlay image (panel on the left) and separate channels (optical sectioning panels) are shown on the right. The cell in the center is transfected with EGFP+ (yellow) and colabeled with Nkx2.1 (green) and Lhx6 (red) in the VZ/SVZ.(B) Transfected EGFP+ cells (green) are colabeled (arrowheads) with GABA (red). The overlay image (panel on the left) and separate channels (optical sectioning) are shown on the right. Insets: drawings of sections with boxed areas presented on photographs.Scale bars represent 50 μm (A) and 20 μm (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4306459&req=5

Figure 6: At P1, Cells Electroporated with hNkx2.1 at E14.5 Express Lhx6 and GABA(A) The overlay image (panel on the left) and separate channels (optical sectioning panels) are shown on the right. The cell in the center is transfected with EGFP+ (yellow) and colabeled with Nkx2.1 (green) and Lhx6 (red) in the VZ/SVZ.(B) Transfected EGFP+ cells (green) are colabeled (arrowheads) with GABA (red). The overlay image (panel on the left) and separate channels (optical sectioning) are shown on the right. Insets: drawings of sections with boxed areas presented on photographs.Scale bars represent 50 μm (A) and 20 μm (B).
Mentions: At P1, the pattern of Nkx2.1 staining in the mouse control brain (Figure S5) was similar to that previously described (Marin et al., 2000). Nkx2.1 expression was restricted to the basal ganglia, while in the cortex only sparse (isolated) Nkx2.1+ cells could be identified, similar to previous reports in neonatal mice (Ohira et al., 2010). In control animals electroporated with (pCAGG-EGFP), transfected cells were not colabeled with the anti-Nkx2.1 antibody and were predominantly located in the upper cortical layers II/III of the cortex and less often in the VZ/SVZ (Figure S5B). In contrast, hNkx2.1/EGFP+ transfected cells were mainly located in the VZ, although individual cells with migratory, bipolar morphology were also demonstrated throughout the cerebral wall, from the ventricular to pial surface (Figure S5C). Immunohistochemical analyses showed that the majority of electroporated (hNkx2.1/EGFP+) cells were colabeled with the antibody to Nkx2.1 (Figure S5C) or Lhx6+ and were distributed in the VZ/SVZ, but rarely in the cortical plate (Figure 6A). In addition, a number of electroporated cells expressed GABA (Figure 6B) and were occasionally labeled with CalR, whereas other interneuron markers, such as PV or Sst, did not colabel cortical cells at P1. This finding indicates that after 7 days of differentiation, ectopic expression of hNkx2.1 gene is sufficient to impart GABAergic-like properties in neurons generated in the mouse dorsal pallium.

Bottom Line: In human and nonhuman primates, evidence suggests that an additional subset of neocortical GABAergic interneurons is generated in the cortical VZ and a proliferative niche, the outer SVZ.The origin, magnitude, and significance of this species-specific difference are not known.Our findings suggest that these progenitors constitute an evolutionary novelty contributing to the elaboration of higher cognitive functions in primates.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030, USA; Institute of Medical and Clinical Biochemistry, School of Medicine, University of Belgrade, Pasterova 2, 11000 Belgrade, Serbia.

Show MeSH
Related in: MedlinePlus