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Simultaneous treatment with statins and aspirin reduces the risk of prostate cancer detection and tumorigenic properties in prostate cancer cell lines.

Olivan M, Rigau M, Colás E, Garcia M, Montes M, Sequeiros T, Regis L, Celma A, Planas J, Placer J, Reventós J, de Torres I, Doll A, Morote J - Biomed Res Int (2015)

Bottom Line: The results showed that a combination of statins and aspirin enhances the effect of individual treatments and seems to reduce the risk of PCa detection (OR: 0.616 (95% CI: 0.467-0.812), P<0.001).However, if treatments are maintained, aspirin (OR: 1.835 (95% CI: 1.068-3.155), P=0.028) or the combination of both drugs (OR: 3.059 (95% CI: 1.894-4.939), P<0.001) represents an increased risk of HGPCa.As observed at clinical level, these beneficial effects in vitro are enhanced when both treatments are administered simultaneously, suggesting that chronic, concomitant treatment with statins and aspirin has a protective effect on PCa incidence.

View Article: PubMed Central - PubMed

Affiliation: Research Unit in Biomedicine and Translational Oncology, Vall d'Hebron Research Institute and Hospital and Autonomous University of Barcelona, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.

ABSTRACT
Nowadays prostate cancer is the most common solid tumor in men from industrialized countries and the second leading cause of death. At the ages when PCa is usually diagnosed, mortality related to cardiovascular morbidity is high; therefore, men at risk for PCa frequently receive chronic lipid-lowering and antiplatelet treatment. The aim of this study was to analyze how chronic treatment with statins, aspirin, and their combination influenced the risk of PCa detection. The tumorigenic properties of these treatments were evaluated by proliferation, colony formation, invasion, and migration assays using different PCa cell lines, in order to assess how these treatments act at molecular level. The results showed that a combination of statins and aspirin enhances the effect of individual treatments and seems to reduce the risk of PCa detection (OR: 0.616 (95% CI: 0.467-0.812), P<0.001). However, if treatments are maintained, aspirin (OR: 1.835 (95% CI: 1.068-3.155), P=0.028) or the combination of both drugs (OR: 3.059 (95% CI: 1.894-4.939), P<0.001) represents an increased risk of HGPCa. As observed at clinical level, these beneficial effects in vitro are enhanced when both treatments are administered simultaneously, suggesting that chronic, concomitant treatment with statins and aspirin has a protective effect on PCa incidence.

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Effect of STA, ASA, and the combination of both treatments on PC3 cell line colony formation capacity. Values for colony formation capacity are presented as crystal violet absorbance/well after 10 days of treatment for PC3 cell line with different conditions: nontreated cells, control (C) and control with DMSO (C(DMSO)), and cells treated with statin (STA), aspirin (ASA), and a combination of both statin and aspirin (STA + ASA) (for further details, see Section 2). (a) The bars represent the mean ± SEM. Values that are significantly different by one-way analysis of variance (ANOVA) from the C(DMSO) group are indicated by **P < 0.01, ***P < 0.001. (b) An inverted microscope with phase contrast at 10x magnification was used to take images of stained colonies.
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fig2: Effect of STA, ASA, and the combination of both treatments on PC3 cell line colony formation capacity. Values for colony formation capacity are presented as crystal violet absorbance/well after 10 days of treatment for PC3 cell line with different conditions: nontreated cells, control (C) and control with DMSO (C(DMSO)), and cells treated with statin (STA), aspirin (ASA), and a combination of both statin and aspirin (STA + ASA) (for further details, see Section 2). (a) The bars represent the mean ± SEM. Values that are significantly different by one-way analysis of variance (ANOVA) from the C(DMSO) group are indicated by **P < 0.01, ***P < 0.001. (b) An inverted microscope with phase contrast at 10x magnification was used to take images of stained colonies.

Mentions: PC3 cells were grown at low cell density (250 cells/well) in the absence or presence of the indicated concentration of STA, ASA, and the combination of both STA and ASA for 10 days. PC3 cells formed colonies, as described by others [30]; however LNCaP cells did not show the same ability to grow from so low cell density. Regarding treatment effects, long-term ASA treatment inhibited cell colony formation ability compared to control cells which had been receiving DMSO in 36% (P value < 0.001); furthermore, this effect was even higher in those cells treated with STA (91%; P value < 0.001) and the combination of both drugs (98%; P value < 0.001) (Figure 2).


Simultaneous treatment with statins and aspirin reduces the risk of prostate cancer detection and tumorigenic properties in prostate cancer cell lines.

Olivan M, Rigau M, Colás E, Garcia M, Montes M, Sequeiros T, Regis L, Celma A, Planas J, Placer J, Reventós J, de Torres I, Doll A, Morote J - Biomed Res Int (2015)

Effect of STA, ASA, and the combination of both treatments on PC3 cell line colony formation capacity. Values for colony formation capacity are presented as crystal violet absorbance/well after 10 days of treatment for PC3 cell line with different conditions: nontreated cells, control (C) and control with DMSO (C(DMSO)), and cells treated with statin (STA), aspirin (ASA), and a combination of both statin and aspirin (STA + ASA) (for further details, see Section 2). (a) The bars represent the mean ± SEM. Values that are significantly different by one-way analysis of variance (ANOVA) from the C(DMSO) group are indicated by **P < 0.01, ***P < 0.001. (b) An inverted microscope with phase contrast at 10x magnification was used to take images of stained colonies.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306438&req=5

fig2: Effect of STA, ASA, and the combination of both treatments on PC3 cell line colony formation capacity. Values for colony formation capacity are presented as crystal violet absorbance/well after 10 days of treatment for PC3 cell line with different conditions: nontreated cells, control (C) and control with DMSO (C(DMSO)), and cells treated with statin (STA), aspirin (ASA), and a combination of both statin and aspirin (STA + ASA) (for further details, see Section 2). (a) The bars represent the mean ± SEM. Values that are significantly different by one-way analysis of variance (ANOVA) from the C(DMSO) group are indicated by **P < 0.01, ***P < 0.001. (b) An inverted microscope with phase contrast at 10x magnification was used to take images of stained colonies.
Mentions: PC3 cells were grown at low cell density (250 cells/well) in the absence or presence of the indicated concentration of STA, ASA, and the combination of both STA and ASA for 10 days. PC3 cells formed colonies, as described by others [30]; however LNCaP cells did not show the same ability to grow from so low cell density. Regarding treatment effects, long-term ASA treatment inhibited cell colony formation ability compared to control cells which had been receiving DMSO in 36% (P value < 0.001); furthermore, this effect was even higher in those cells treated with STA (91%; P value < 0.001) and the combination of both drugs (98%; P value < 0.001) (Figure 2).

Bottom Line: The results showed that a combination of statins and aspirin enhances the effect of individual treatments and seems to reduce the risk of PCa detection (OR: 0.616 (95% CI: 0.467-0.812), P<0.001).However, if treatments are maintained, aspirin (OR: 1.835 (95% CI: 1.068-3.155), P=0.028) or the combination of both drugs (OR: 3.059 (95% CI: 1.894-4.939), P<0.001) represents an increased risk of HGPCa.As observed at clinical level, these beneficial effects in vitro are enhanced when both treatments are administered simultaneously, suggesting that chronic, concomitant treatment with statins and aspirin has a protective effect on PCa incidence.

View Article: PubMed Central - PubMed

Affiliation: Research Unit in Biomedicine and Translational Oncology, Vall d'Hebron Research Institute and Hospital and Autonomous University of Barcelona, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.

ABSTRACT
Nowadays prostate cancer is the most common solid tumor in men from industrialized countries and the second leading cause of death. At the ages when PCa is usually diagnosed, mortality related to cardiovascular morbidity is high; therefore, men at risk for PCa frequently receive chronic lipid-lowering and antiplatelet treatment. The aim of this study was to analyze how chronic treatment with statins, aspirin, and their combination influenced the risk of PCa detection. The tumorigenic properties of these treatments were evaluated by proliferation, colony formation, invasion, and migration assays using different PCa cell lines, in order to assess how these treatments act at molecular level. The results showed that a combination of statins and aspirin enhances the effect of individual treatments and seems to reduce the risk of PCa detection (OR: 0.616 (95% CI: 0.467-0.812), P<0.001). However, if treatments are maintained, aspirin (OR: 1.835 (95% CI: 1.068-3.155), P=0.028) or the combination of both drugs (OR: 3.059 (95% CI: 1.894-4.939), P<0.001) represents an increased risk of HGPCa. As observed at clinical level, these beneficial effects in vitro are enhanced when both treatments are administered simultaneously, suggesting that chronic, concomitant treatment with statins and aspirin has a protective effect on PCa incidence.

Show MeSH
Related in: MedlinePlus