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Protective effect of sodium nitroprusside on the rat small intestine transplanted mucosa.

Chen FH, Li K, Yin L, Chen CQ, Yan ZW, Chen GM - Biochem Res Int (2015)

Bottom Line: Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples.In addition, mucosal samples from the pre-SNP group showed higher Na(+)-K(+)-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples.The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.

View Article: PubMed Central - PubMed

Affiliation: Ultrasound Department, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China.

ABSTRACT
The intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (SBT). Previous studies have demonstrated a protective effect of nitric oxide (NO) on other transplanted organs and NO mediated intestinal protection has also been reported in vitro. The aim of this study was to evaluate the effect of sodium nitroprusside (SNP), NO donor, on graft mucosal histology and molecular markers of function after SBT in rats. We used SNP in different period of heterotopic SBT rats. The groups consisted of SBT, pre-SNP group, and post-SNP group. Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples. In addition, mucosal samples from the pre-SNP group showed higher Na(+)-K(+)-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples. The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.

No MeSH data available.


Related in: MedlinePlus

Lamininhybridization in situ. Mucosal laminin mRNA is expressed in the basal part of the intestinal mucosa. A representative sample from the SBT group (n = 12): mucosal laminin mRNA expression is low with sections of an interrupted expression. (b) A representative sample from the pre-SNP group (n = 14): the expression is continuous. (c) Post-SNP group (n = 16): the expression is low and there are interruptions present (×200).
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fig7: Lamininhybridization in situ. Mucosal laminin mRNA is expressed in the basal part of the intestinal mucosa. A representative sample from the SBT group (n = 12): mucosal laminin mRNA expression is low with sections of an interrupted expression. (b) A representative sample from the pre-SNP group (n = 14): the expression is continuous. (c) Post-SNP group (n = 16): the expression is low and there are interruptions present (×200).

Mentions: As shown in Figures 5, 6, and 7, the pre-SNP group exhibited a higher expression level of laminin as quantified by semiquantitative RT-PCR in situ hybridization and protein immunohistochemistry compared to the SBT and post-SNP groups. Laminin protein expression examined by immunohistochemistry was stronger in the pre-SNP group compared to the SBT and post-SNP groups. Experiments with hybridization and immunohistochemistry revealed that the laminin expression was localized to the mucosal epithelial basement membrane. There was no difference in laminin expression between the SBT and post-SNP groups.


Protective effect of sodium nitroprusside on the rat small intestine transplanted mucosa.

Chen FH, Li K, Yin L, Chen CQ, Yan ZW, Chen GM - Biochem Res Int (2015)

Lamininhybridization in situ. Mucosal laminin mRNA is expressed in the basal part of the intestinal mucosa. A representative sample from the SBT group (n = 12): mucosal laminin mRNA expression is low with sections of an interrupted expression. (b) A representative sample from the pre-SNP group (n = 14): the expression is continuous. (c) Post-SNP group (n = 16): the expression is low and there are interruptions present (×200).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4306256&req=5

fig7: Lamininhybridization in situ. Mucosal laminin mRNA is expressed in the basal part of the intestinal mucosa. A representative sample from the SBT group (n = 12): mucosal laminin mRNA expression is low with sections of an interrupted expression. (b) A representative sample from the pre-SNP group (n = 14): the expression is continuous. (c) Post-SNP group (n = 16): the expression is low and there are interruptions present (×200).
Mentions: As shown in Figures 5, 6, and 7, the pre-SNP group exhibited a higher expression level of laminin as quantified by semiquantitative RT-PCR in situ hybridization and protein immunohistochemistry compared to the SBT and post-SNP groups. Laminin protein expression examined by immunohistochemistry was stronger in the pre-SNP group compared to the SBT and post-SNP groups. Experiments with hybridization and immunohistochemistry revealed that the laminin expression was localized to the mucosal epithelial basement membrane. There was no difference in laminin expression between the SBT and post-SNP groups.

Bottom Line: Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples.In addition, mucosal samples from the pre-SNP group showed higher Na(+)-K(+)-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples.The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.

View Article: PubMed Central - PubMed

Affiliation: Ultrasound Department, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China.

ABSTRACT
The intestinal mucosal epithelium is extremely susceptible to even brief periods of ischemia. Mucosal barrier damage, which is associated with ischemia/reperfusion (I/R) injury and consequently bacterial translocation, remains a major obstacle for clinically successful small bowel transplantation (SBT). Previous studies have demonstrated a protective effect of nitric oxide (NO) on other transplanted organs and NO mediated intestinal protection has also been reported in vitro. The aim of this study was to evaluate the effect of sodium nitroprusside (SNP), NO donor, on graft mucosal histology and molecular markers of function after SBT in rats. We used SNP in different period of heterotopic SBT rats. The groups consisted of SBT, pre-SNP group, and post-SNP group. Interestingly, the pre-SNP graft samples exhibited less damage compared to the SBT and post-SNP samples. In addition, mucosal samples from the pre-SNP group showed higher Na(+)-K(+)-ATPase activity and higher levels of laminin expression compared to the SBT and post-SNP samples. The findings of the present study reveal that SNP given before graft ischemia/reperfusion injury has a protective effect on mucosal histology and molecular markers of function in the transplanted small intestine.

No MeSH data available.


Related in: MedlinePlus