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Transient middle cerebral artery occlusion with complete reperfusion in spontaneously hypertensive rats.

Hill JW, Nemoto EM - MethodsX (2014)

Bottom Line: Middle cerebral artery occlusion (MCAO) by the intraluminal suture method is widely used to model ischemic stroke in rats.Current methods include transection or ligation of the external carotid or common carotid artery and thus result in partial restoration of perfusion after transient MCAO.Advantages of the method include: MCAO is achieved through insertion of an intraluminal suture into the internal carotid artery through the common carotid artery.At the end of the occlusion period, the suture is withdrawn and the incision in the common carotid artery is closed with cyanoacrylate tissue adhesive and complete reperfusion is established.No residual subcutaneous sutures remain during recovery.Vasculature is restored to the preoperative state.

View Article: PubMed Central - PubMed

Affiliation: University of New Mexico Health Sciences Center, Department of Neurosurgery, Albuquerque, New Mexico, 87131.

ABSTRACT

Middle cerebral artery occlusion (MCAO) by the intraluminal suture method is widely used to model ischemic stroke in rats. Current methods include transection or ligation of the external carotid or common carotid artery and thus result in partial restoration of perfusion after transient MCAO. Since incomplete reperfusion may influence recovery and thus confound studies of the impact of neuroprotective compounds and therapies on outcomes after stroke, we have devised a novel method to induce transient MCAO with complete reperfusion. Advantages of the method include: MCAO is achieved through insertion of an intraluminal suture into the internal carotid artery through the common carotid artery.At the end of the occlusion period, the suture is withdrawn and the incision in the common carotid artery is closed with cyanoacrylate tissue adhesive and complete reperfusion is established.No residual subcutaneous sutures remain during recovery.Vasculature is restored to the preoperative state.

No MeSH data available.


Related in: MedlinePlus

Complete closure of the CCA after a second application of adhesive. The cyanoacrylate patch is indicated with an arrow.
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fig0045: Complete closure of the CCA after a second application of adhesive. The cyanoacrylate patch is indicated with an arrow.

Mentions: After 90 min, the animal is reanesthetized and the surgical field exposed. The upper suture is loosened and the occluding suture is withdrawn to the middle suture. The upper suture is moved rostrally and secured around the CCA above the silicone tip of the occluding suture. The middle and occluding sutures are removed and the CCA is clamped 1 mm rostral to the incision in the CCA (Fig. 6). In this position, the incision is visible on the surface of the CCA and the CCA lumen is closed. A small drop of tissue adhesive is applied to the back of a 25G needle by placing the tip of the needle bevel up in a drop of adhesive. The proper amount of adhesive (an approximately 0.25 mm thick drop) can be verified through the microscope before application to the incision (Fig. 7). The adhesive is applied directly onto the incision (Fig. 8) and the clamp is removed after 1 min. Using a clean 25G needle, a second drop of adhesive is applied around the perimeter of the first application to create a patch approximately 0.5 mm in diameter (Fig. 9). The adhesive is allowed to cure for 2 min. The rostral silk suture around the CCA is loosened and the patch is observed for leakage. If leakage around the patch is detected, the rostral silk suture is resecured and the patch is expanded in the affected area with a minimal application of adhesive. If the patch is secure, the lower suture is carefully removed and perfusion through the CCA is verified (Fig. 10). After complete reperfusion is achieved (Fig. 11), the wound is closed and the animal is given 3 ml of subcutaneous normal saline to prevent dehydration.


Transient middle cerebral artery occlusion with complete reperfusion in spontaneously hypertensive rats.

Hill JW, Nemoto EM - MethodsX (2014)

Complete closure of the CCA after a second application of adhesive. The cyanoacrylate patch is indicated with an arrow.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306192&req=5

fig0045: Complete closure of the CCA after a second application of adhesive. The cyanoacrylate patch is indicated with an arrow.
Mentions: After 90 min, the animal is reanesthetized and the surgical field exposed. The upper suture is loosened and the occluding suture is withdrawn to the middle suture. The upper suture is moved rostrally and secured around the CCA above the silicone tip of the occluding suture. The middle and occluding sutures are removed and the CCA is clamped 1 mm rostral to the incision in the CCA (Fig. 6). In this position, the incision is visible on the surface of the CCA and the CCA lumen is closed. A small drop of tissue adhesive is applied to the back of a 25G needle by placing the tip of the needle bevel up in a drop of adhesive. The proper amount of adhesive (an approximately 0.25 mm thick drop) can be verified through the microscope before application to the incision (Fig. 7). The adhesive is applied directly onto the incision (Fig. 8) and the clamp is removed after 1 min. Using a clean 25G needle, a second drop of adhesive is applied around the perimeter of the first application to create a patch approximately 0.5 mm in diameter (Fig. 9). The adhesive is allowed to cure for 2 min. The rostral silk suture around the CCA is loosened and the patch is observed for leakage. If leakage around the patch is detected, the rostral silk suture is resecured and the patch is expanded in the affected area with a minimal application of adhesive. If the patch is secure, the lower suture is carefully removed and perfusion through the CCA is verified (Fig. 10). After complete reperfusion is achieved (Fig. 11), the wound is closed and the animal is given 3 ml of subcutaneous normal saline to prevent dehydration.

Bottom Line: Middle cerebral artery occlusion (MCAO) by the intraluminal suture method is widely used to model ischemic stroke in rats.Current methods include transection or ligation of the external carotid or common carotid artery and thus result in partial restoration of perfusion after transient MCAO.Advantages of the method include: MCAO is achieved through insertion of an intraluminal suture into the internal carotid artery through the common carotid artery.At the end of the occlusion period, the suture is withdrawn and the incision in the common carotid artery is closed with cyanoacrylate tissue adhesive and complete reperfusion is established.No residual subcutaneous sutures remain during recovery.Vasculature is restored to the preoperative state.

View Article: PubMed Central - PubMed

Affiliation: University of New Mexico Health Sciences Center, Department of Neurosurgery, Albuquerque, New Mexico, 87131.

ABSTRACT

Middle cerebral artery occlusion (MCAO) by the intraluminal suture method is widely used to model ischemic stroke in rats. Current methods include transection or ligation of the external carotid or common carotid artery and thus result in partial restoration of perfusion after transient MCAO. Since incomplete reperfusion may influence recovery and thus confound studies of the impact of neuroprotective compounds and therapies on outcomes after stroke, we have devised a novel method to induce transient MCAO with complete reperfusion. Advantages of the method include: MCAO is achieved through insertion of an intraluminal suture into the internal carotid artery through the common carotid artery.At the end of the occlusion period, the suture is withdrawn and the incision in the common carotid artery is closed with cyanoacrylate tissue adhesive and complete reperfusion is established.No residual subcutaneous sutures remain during recovery.Vasculature is restored to the preoperative state.

No MeSH data available.


Related in: MedlinePlus