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Manganese superoxide dismutase is required to maintain osteoclast differentiation and function under static force.

Guo T, Zhang L, Konermann A, Zhou H, Jin F, Liu W - Sci Rep (2015)

Bottom Line: Total respective protein spot numbers of 549 ± 13, 612 ± 19 and 634 ± 16 were detected in each of the gels by image analysis.The five proteins identified were plasminogen activator inhibitor 2 (PAI-2, Spot 1), peroxiredoxin-6 (PRD-6, Spot 3), manganese superoxide dismutase (SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (L-LDH, Spot 15).More importantly, we revealed that SOD2 was required to maintain monocyte differentiation into functional OCs and may become a potential target for regulating the efficiency of OTM in the future.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, People's Republic of China.

ABSTRACT
Bone homeostasis is maintained by the balance of osteoblasts (OBs) and osteoclasts (OCs). Increased activity of OCs not only contributes to pathological bone resorption, such as osteoporosis and periodontitis, but also is responsible for physiological conditions like orthodontic tooth movement (OTM). However, the detailed mechanism by which orthodontic force promotes the formation of OCs is still poorly understood. In this study, we confirmed that static force promoted the differentiation of human cord monocytes (HMNCs) into OCs depending on loading time and magnitude. Protein expression profiles among HMNCs, HMNCs subjected to static force and mature OCs were established via 2-DE and MALDI-TOF-MS analyses. Total respective protein spot numbers of 549 ± 13, 612 ± 19 and 634 ± 16 were detected in each of the gels by image analysis. The five proteins identified were plasminogen activator inhibitor 2 (PAI-2, Spot 1), peroxiredoxin-6 (PRD-6, Spot 3), manganese superoxide dismutase (SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (L-LDH, Spot 15). More importantly, we revealed that SOD2 was required to maintain monocyte differentiation into functional OCs and may become a potential target for regulating the efficiency of OTM in the future.

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Peptide fingerprint mass spectra analyses of key proteins.(A)–(E), The five key proteins plasminogen activator inhibitor 2 (A: PAI-2, Spot 1), peroxiredoxin-6 (B: PRD-6, Spot 3), manganese superoxide dismutase (C: SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (D: Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (E: L-LDH, Spot 15) identified via 2-DE maps were trypsin digested and evaluated by mass spectrometry.
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f4: Peptide fingerprint mass spectra analyses of key proteins.(A)–(E), The five key proteins plasminogen activator inhibitor 2 (A: PAI-2, Spot 1), peroxiredoxin-6 (B: PRD-6, Spot 3), manganese superoxide dismutase (C: SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (D: Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (E: L-LDH, Spot 15) identified via 2-DE maps were trypsin digested and evaluated by mass spectrometry.

Mentions: A total of nine 2-DE experiments were performed for three HMNCs control samples, three HMNC samples with 150 kpa static force application for 1.5 h and three induced-mature OC samples. For these groups, total protein spot numbers of 549 ± 13, 612 ± 19 and 634 ± 16, respectively, were detected in each of the gels over a pH range of 3 to 10 by image analysis (Fig. 3a–c). The comparative analysis of the normalized volumes of each spot revealed that a total of five spots were differentially expressed among the groups. Three spots were up-regulated after static force loading, and two spots were only expressed in mature OCs (Fig. 3d). An ESI-Q-TOF MS/MS analyzer was used for accurate identification of the proteins, and the mass identification information for the five key proteins is listed in Table 1. The five proteins were identified as plasminogen activator inhibitor 2 (PAI-2, Spot 1), peroxiredoxin-6 (PRD-6, Spot 3), manganese superoxide dismutase (SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (L-LDH, Spot 15) (Fig. 4).


Manganese superoxide dismutase is required to maintain osteoclast differentiation and function under static force.

Guo T, Zhang L, Konermann A, Zhou H, Jin F, Liu W - Sci Rep (2015)

Peptide fingerprint mass spectra analyses of key proteins.(A)–(E), The five key proteins plasminogen activator inhibitor 2 (A: PAI-2, Spot 1), peroxiredoxin-6 (B: PRD-6, Spot 3), manganese superoxide dismutase (C: SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (D: Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (E: L-LDH, Spot 15) identified via 2-DE maps were trypsin digested and evaluated by mass spectrometry.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306132&req=5

f4: Peptide fingerprint mass spectra analyses of key proteins.(A)–(E), The five key proteins plasminogen activator inhibitor 2 (A: PAI-2, Spot 1), peroxiredoxin-6 (B: PRD-6, Spot 3), manganese superoxide dismutase (C: SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (D: Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (E: L-LDH, Spot 15) identified via 2-DE maps were trypsin digested and evaluated by mass spectrometry.
Mentions: A total of nine 2-DE experiments were performed for three HMNCs control samples, three HMNC samples with 150 kpa static force application for 1.5 h and three induced-mature OC samples. For these groups, total protein spot numbers of 549 ± 13, 612 ± 19 and 634 ± 16, respectively, were detected in each of the gels over a pH range of 3 to 10 by image analysis (Fig. 3a–c). The comparative analysis of the normalized volumes of each spot revealed that a total of five spots were differentially expressed among the groups. Three spots were up-regulated after static force loading, and two spots were only expressed in mature OCs (Fig. 3d). An ESI-Q-TOF MS/MS analyzer was used for accurate identification of the proteins, and the mass identification information for the five key proteins is listed in Table 1. The five proteins were identified as plasminogen activator inhibitor 2 (PAI-2, Spot 1), peroxiredoxin-6 (PRD-6, Spot 3), manganese superoxide dismutase (SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (L-LDH, Spot 15) (Fig. 4).

Bottom Line: Total respective protein spot numbers of 549 ± 13, 612 ± 19 and 634 ± 16 were detected in each of the gels by image analysis.The five proteins identified were plasminogen activator inhibitor 2 (PAI-2, Spot 1), peroxiredoxin-6 (PRD-6, Spot 3), manganese superoxide dismutase (SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (L-LDH, Spot 15).More importantly, we revealed that SOD2 was required to maintain monocyte differentiation into functional OCs and may become a potential target for regulating the efficiency of OTM in the future.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, People's Republic of China.

ABSTRACT
Bone homeostasis is maintained by the balance of osteoblasts (OBs) and osteoclasts (OCs). Increased activity of OCs not only contributes to pathological bone resorption, such as osteoporosis and periodontitis, but also is responsible for physiological conditions like orthodontic tooth movement (OTM). However, the detailed mechanism by which orthodontic force promotes the formation of OCs is still poorly understood. In this study, we confirmed that static force promoted the differentiation of human cord monocytes (HMNCs) into OCs depending on loading time and magnitude. Protein expression profiles among HMNCs, HMNCs subjected to static force and mature OCs were established via 2-DE and MALDI-TOF-MS analyses. Total respective protein spot numbers of 549 ± 13, 612 ± 19 and 634 ± 16 were detected in each of the gels by image analysis. The five proteins identified were plasminogen activator inhibitor 2 (PAI-2, Spot 1), peroxiredoxin-6 (PRD-6, Spot 3), manganese superoxide dismutase (SOD2, Spot 6), Rho GDP-dissociation inhibitor 2 (Rho-GDI2, Spot 11) and L-lactate dehydrogenase B chain (L-LDH, Spot 15). More importantly, we revealed that SOD2 was required to maintain monocyte differentiation into functional OCs and may become a potential target for regulating the efficiency of OTM in the future.

Show MeSH
Related in: MedlinePlus