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Characterising human atherosclerotic carotid plaque tissue composition and morphology using combined spectroscopic and imaging modalities.

Barrett HE, Mulvihill JJ, Cunnane EM, Walsh MT - Biomed Eng Online (2015)

Bottom Line: The tissue characterisation processes were then applied to the mechanical material plaque properties acquired from experimental circumferential loading of human carotid plaque specimen for comparison of the methods.FTIR characterised the degree of plaque progression by identifying the functional groups associated with lipid, collagen and calcification in each specimen.This identified a negative relationship between stiffness and 'lipid to collagen' and 'calcification to collagen' ratios.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Calcification is a marked pathological component in carotid artery plaque. Studies have suggested that calcification may induce regions of high stress concentrations therefore increasing the potential for rupture. However, the mechanical behaviour of the plaque under the influence of calcification is not fully understood. A method of accurately characterising the calcification coupled with the associated mechanical plaque properties is needed to better understand the impact of calcification on the mechanical behaviour of the plaque during minimally invasive treatments. This study proposes a comparison of biochemical and structural characterisation methods of the calcification in carotid plaque specimens to identify plaque mechanical behaviour. Biochemical analysis, by Fourier Transform Infrared (FTIR) spectroscopy, was used to identify the key components, including calcification, in each plaque sample. However, FTIR has a finite penetration depth which may limit the accuracy of the calcification measurement. Therefore, this FTIR analysis was coupled with the identification of the calcification inclusions located internally in the plaque specimen using micro x-ray computed tomography (μX-CT) which measures the calcification volume fraction (CVF) to total tissue content. The tissue characterisation processes were then applied to the mechanical material plaque properties acquired from experimental circumferential loading of human carotid plaque specimen for comparison of the methods. FTIR characterised the degree of plaque progression by identifying the functional groups associated with lipid, collagen and calcification in each specimen. This identified a negative relationship between stiffness and 'lipid to collagen' and 'calcification to collagen' ratios. However, μX-CT results suggest that CVF measurements relate to overall mechanical stiffness, while peak circumferential strength values may be dependent on specific calcification geometries. This study demonstrates the need to fully characterise the calcification structure of the plaque tissue and that a combination of FTIR and μX-CT provides the necessary information to fully understand the mechanical behaviour of the plaque tissue.

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Cauchy stress and stretch ratio plots of the plaque samples grouped by initial stiffness. The dark grey represents high stiffness (HS), the dashed line represents medium stiffness (MS) and the light grey represents low stiffness (LS). Note Mechanical data extracted from Mulvihill et al. 2013.
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Figure 2: Cauchy stress and stretch ratio plots of the plaque samples grouped by initial stiffness. The dark grey represents high stiffness (HS), the dashed line represents medium stiffness (MS) and the light grey represents low stiffness (LS). Note Mechanical data extracted from Mulvihill et al. 2013.

Mentions: Table 1 summarises the structural µX-CT results for each carotid specimen along with the previously published biochemical and experimental mechanical data [5]. This sample set is tabulated in order of increasing (low to high) initial stiffness (MPa). The Cauchy stress and stretch ratio response to the uniaxial testing of whole human carotid plaque samples in the circumferential loading direction is presented in Figure 2. The heterogeneous morphology of each plaque is demonstrated by the large inter-specimen disparity in mechanical behaviour. Three distinct stiffness levels were exhibited and each plaque was grouped based on their respective level of initial stiffness. The colour of the lines (light grey; grey dashed and dark grey) indicates the three mechanical stiffness levels (LS, low stiffness; MS, medium stiffness and HS, high stiffness) respectively. The stress and stretch data quantify the stress induced on the plaque tissue structure during the large deformation. The mean peak circumferential strength value (± standard deviation) was Cauchy stress 0.40 ± 0.09 MPa and stretch ratio 1.44 ± 0.13.


Characterising human atherosclerotic carotid plaque tissue composition and morphology using combined spectroscopic and imaging modalities.

Barrett HE, Mulvihill JJ, Cunnane EM, Walsh MT - Biomed Eng Online (2015)

Cauchy stress and stretch ratio plots of the plaque samples grouped by initial stiffness. The dark grey represents high stiffness (HS), the dashed line represents medium stiffness (MS) and the light grey represents low stiffness (LS). Note Mechanical data extracted from Mulvihill et al. 2013.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4306117&req=5

Figure 2: Cauchy stress and stretch ratio plots of the plaque samples grouped by initial stiffness. The dark grey represents high stiffness (HS), the dashed line represents medium stiffness (MS) and the light grey represents low stiffness (LS). Note Mechanical data extracted from Mulvihill et al. 2013.
Mentions: Table 1 summarises the structural µX-CT results for each carotid specimen along with the previously published biochemical and experimental mechanical data [5]. This sample set is tabulated in order of increasing (low to high) initial stiffness (MPa). The Cauchy stress and stretch ratio response to the uniaxial testing of whole human carotid plaque samples in the circumferential loading direction is presented in Figure 2. The heterogeneous morphology of each plaque is demonstrated by the large inter-specimen disparity in mechanical behaviour. Three distinct stiffness levels were exhibited and each plaque was grouped based on their respective level of initial stiffness. The colour of the lines (light grey; grey dashed and dark grey) indicates the three mechanical stiffness levels (LS, low stiffness; MS, medium stiffness and HS, high stiffness) respectively. The stress and stretch data quantify the stress induced on the plaque tissue structure during the large deformation. The mean peak circumferential strength value (± standard deviation) was Cauchy stress 0.40 ± 0.09 MPa and stretch ratio 1.44 ± 0.13.

Bottom Line: The tissue characterisation processes were then applied to the mechanical material plaque properties acquired from experimental circumferential loading of human carotid plaque specimen for comparison of the methods.FTIR characterised the degree of plaque progression by identifying the functional groups associated with lipid, collagen and calcification in each specimen.This identified a negative relationship between stiffness and 'lipid to collagen' and 'calcification to collagen' ratios.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
Calcification is a marked pathological component in carotid artery plaque. Studies have suggested that calcification may induce regions of high stress concentrations therefore increasing the potential for rupture. However, the mechanical behaviour of the plaque under the influence of calcification is not fully understood. A method of accurately characterising the calcification coupled with the associated mechanical plaque properties is needed to better understand the impact of calcification on the mechanical behaviour of the plaque during minimally invasive treatments. This study proposes a comparison of biochemical and structural characterisation methods of the calcification in carotid plaque specimens to identify plaque mechanical behaviour. Biochemical analysis, by Fourier Transform Infrared (FTIR) spectroscopy, was used to identify the key components, including calcification, in each plaque sample. However, FTIR has a finite penetration depth which may limit the accuracy of the calcification measurement. Therefore, this FTIR analysis was coupled with the identification of the calcification inclusions located internally in the plaque specimen using micro x-ray computed tomography (μX-CT) which measures the calcification volume fraction (CVF) to total tissue content. The tissue characterisation processes were then applied to the mechanical material plaque properties acquired from experimental circumferential loading of human carotid plaque specimen for comparison of the methods. FTIR characterised the degree of plaque progression by identifying the functional groups associated with lipid, collagen and calcification in each specimen. This identified a negative relationship between stiffness and 'lipid to collagen' and 'calcification to collagen' ratios. However, μX-CT results suggest that CVF measurements relate to overall mechanical stiffness, while peak circumferential strength values may be dependent on specific calcification geometries. This study demonstrates the need to fully characterise the calcification structure of the plaque tissue and that a combination of FTIR and μX-CT provides the necessary information to fully understand the mechanical behaviour of the plaque tissue.

Show MeSH
Related in: MedlinePlus