Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC.
Bottom Line: To define these cell types more rigorously, we have performed comparative microarray analysis to describe a transcriptomic profile specific for each cell type.This is validated by real time RT-PCR and in situ hybridisation.We find that both cES and cBC cells express classic pluripotency-related genes (including cPOUV/OCT4, NANOG, SOX2/3, KLF2 and SALL4), whereas expression of DAZL, DND1, DDX4 and PIWIL1 defines a molecular signature for germ cells.
Affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France.Show MeSH
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Mentions: The transcriptomic landscape between different chicken stem cells and fibroblasts was established using the most recent version of commercially annotated microarrays. For each cell type, three independent samples were hybridised and a first PCA analysis obtained from all probe sets demonstrates the molecular differences between the cell types analysed (Fig. 1A). Using cutoffs of p-value ≤ 0.05 and Log2(FC) ≥ 2, genes presenting at least one difference in expression between the different cell types were selected (Table S2). The genes were classified as either up-regulated (U), down-regulated (D) or not differentially expressed (n) between the different cells. A total of 5944 differentially expressed unique identifiers (target IDs) were defined for CEF, 5942 for BM2 cells, 4550 for cES, 5465 for PGC and 5522 for cBC.
Affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France.