Limits...
Dietary Enterococcus faecalis LAB31 improves growth performance, reduces diarrhea, and increases fecal Lactobacillus number of weaned piglets.

Hu Y, Dun Y, Li S, Zhang D, Peng N, Zhao S, Liang Y - PLoS ONE (2015)

Bottom Line: Denaturing gradient gel electrophoresis (DGGE) showed that the application of probiotics to the diet changed the bacterial community, with a higher bacterial diversity in group M than in the other four groups.Real-time PCR revealed that the relative number of Lactobacillus increased by addition of probiotics, and was higher in group H than in group N (P0 < 0.05).However, group-specific PCR-DGGE showed no obvious difference among the five groups in Lactobacillus composition and diversity.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China; College of Life Sciences, Hubei Normal University, Huangshi, Hubei 435002, P.R. China.

ABSTRACT
Lactic acid bacteria (LAB) have been shown to enhance performance of weaned piglets. However, few studies have reported the addition of LAB Enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. This study evaluated the effects of dietary E. faecalis LAB31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and Lactobacillus communities in weaned piglets. A total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to 5 groups (20 pens, with 4 pens for each group) for a trial of 28 days: group N (negative control, without antibiotics or probiotics); group P (Neomycin sulfate, 100 mg/kg feed); groups L, M and H (supplemented with E. faecalis LAB31 0.5×109, 1.0×109, and 2.5×109 CFU/kg feed, respectively). Average daily gain and feed conversion efficiency were found to be higher in group H than in group N, and showed significant differences between group H and group P (P0 < 0.05). Furthermore, groups H and P had a lower diarrhea index than the other three groups (P0 < 0.05). Denaturing gradient gel electrophoresis (DGGE) showed that the application of probiotics to the diet changed the bacterial community, with a higher bacterial diversity in group M than in the other four groups. Real-time PCR revealed that the relative number of Lactobacillus increased by addition of probiotics, and was higher in group H than in group N (P0 < 0.05). However, group-specific PCR-DGGE showed no obvious difference among the five groups in Lactobacillus composition and diversity. Therefore, the dietary addition of E. faecalis LAB31 can improve growth performance, reduce diarrhea, and increase the relative number of Lactobacillus in feces of weaned piglets.

No MeSH data available.


Related in: MedlinePlus

Lactobacillus community of weaned piglets fed with neomycin or E. faecalis.(A) DGGE profiles of V3 region of the 16S rDNA gene fragments with the primes Lac1 and Lac2-GC. The denaturant gradient range is from 41% to 60%. Lanes N (negative control, basal diet); P (positive control, diet supplemented with neomycin); L, M, H (diets supplemented with probiotics 0.5×109, 1.0×109 and 2.5×109 CFU/kg feed, respectively); (B) UPGMA cluster analysis of Dice similarity indices from DGGE profiles.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4305361&req=5

pone.0116635.g002: Lactobacillus community of weaned piglets fed with neomycin or E. faecalis.(A) DGGE profiles of V3 region of the 16S rDNA gene fragments with the primes Lac1 and Lac2-GC. The denaturant gradient range is from 41% to 60%. Lanes N (negative control, basal diet); P (positive control, diet supplemented with neomycin); L, M, H (diets supplemented with probiotics 0.5×109, 1.0×109 and 2.5×109 CFU/kg feed, respectively); (B) UPGMA cluster analysis of Dice similarity indices from DGGE profiles.

Mentions: No significant difference was shown in Lactobacillus composition and diversity by PCR-DGGE (Fig. 2, Table 8). However, the Shannon index (H′) and Simpson index (1/D) of groups L, M and H showed a slightly higher Lactobacillus diversity than groups N and P. As shown in Fig. 2A, the relative intensity of the dominating bands and the total number of bands in each lane were similar in all 5 groups, with fewer bands in lane P (11 bands) and more bands in lane H (15 bands). The UPGMA cluster analysis showed that groups P and N had a 92% similarity in the Lactobacillus community structure (Fig. 2B).


Dietary Enterococcus faecalis LAB31 improves growth performance, reduces diarrhea, and increases fecal Lactobacillus number of weaned piglets.

Hu Y, Dun Y, Li S, Zhang D, Peng N, Zhao S, Liang Y - PLoS ONE (2015)

Lactobacillus community of weaned piglets fed with neomycin or E. faecalis.(A) DGGE profiles of V3 region of the 16S rDNA gene fragments with the primes Lac1 and Lac2-GC. The denaturant gradient range is from 41% to 60%. Lanes N (negative control, basal diet); P (positive control, diet supplemented with neomycin); L, M, H (diets supplemented with probiotics 0.5×109, 1.0×109 and 2.5×109 CFU/kg feed, respectively); (B) UPGMA cluster analysis of Dice similarity indices from DGGE profiles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4305361&req=5

pone.0116635.g002: Lactobacillus community of weaned piglets fed with neomycin or E. faecalis.(A) DGGE profiles of V3 region of the 16S rDNA gene fragments with the primes Lac1 and Lac2-GC. The denaturant gradient range is from 41% to 60%. Lanes N (negative control, basal diet); P (positive control, diet supplemented with neomycin); L, M, H (diets supplemented with probiotics 0.5×109, 1.0×109 and 2.5×109 CFU/kg feed, respectively); (B) UPGMA cluster analysis of Dice similarity indices from DGGE profiles.
Mentions: No significant difference was shown in Lactobacillus composition and diversity by PCR-DGGE (Fig. 2, Table 8). However, the Shannon index (H′) and Simpson index (1/D) of groups L, M and H showed a slightly higher Lactobacillus diversity than groups N and P. As shown in Fig. 2A, the relative intensity of the dominating bands and the total number of bands in each lane were similar in all 5 groups, with fewer bands in lane P (11 bands) and more bands in lane H (15 bands). The UPGMA cluster analysis showed that groups P and N had a 92% similarity in the Lactobacillus community structure (Fig. 2B).

Bottom Line: Denaturing gradient gel electrophoresis (DGGE) showed that the application of probiotics to the diet changed the bacterial community, with a higher bacterial diversity in group M than in the other four groups.Real-time PCR revealed that the relative number of Lactobacillus increased by addition of probiotics, and was higher in group H than in group N (P0 < 0.05).However, group-specific PCR-DGGE showed no obvious difference among the five groups in Lactobacillus composition and diversity.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China; College of Life Sciences, Hubei Normal University, Huangshi, Hubei 435002, P.R. China.

ABSTRACT
Lactic acid bacteria (LAB) have been shown to enhance performance of weaned piglets. However, few studies have reported the addition of LAB Enterococcus faecalis as alternatives to growth promoting antibiotics for weaned piglets. This study evaluated the effects of dietary E. faecalis LAB31 on the growth performance, diarrhea incidence, blood parameters, fecal bacterial and Lactobacillus communities in weaned piglets. A total of 360 piglets weaned at 26 ± 2 days of age were randomly allotted to 5 groups (20 pens, with 4 pens for each group) for a trial of 28 days: group N (negative control, without antibiotics or probiotics); group P (Neomycin sulfate, 100 mg/kg feed); groups L, M and H (supplemented with E. faecalis LAB31 0.5×109, 1.0×109, and 2.5×109 CFU/kg feed, respectively). Average daily gain and feed conversion efficiency were found to be higher in group H than in group N, and showed significant differences between group H and group P (P0 < 0.05). Furthermore, groups H and P had a lower diarrhea index than the other three groups (P0 < 0.05). Denaturing gradient gel electrophoresis (DGGE) showed that the application of probiotics to the diet changed the bacterial community, with a higher bacterial diversity in group M than in the other four groups. Real-time PCR revealed that the relative number of Lactobacillus increased by addition of probiotics, and was higher in group H than in group N (P0 < 0.05). However, group-specific PCR-DGGE showed no obvious difference among the five groups in Lactobacillus composition and diversity. Therefore, the dietary addition of E. faecalis LAB31 can improve growth performance, reduce diarrhea, and increase the relative number of Lactobacillus in feces of weaned piglets.

No MeSH data available.


Related in: MedlinePlus