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A novel immune competent murine hypertrophic scar contracture model: a tool to elucidate disease mechanism and develop new therapies.

Ibrahim MM, Bond J, Bergeron A, Miller KJ, Ehanire T, Quiles C, Lorden ER, Medina MA, Fisher M, Klitzman B, Selim MA, Leong KW, Levinson H - Wound Repair Regen (2015)

Bottom Line: Current therapies are marginally effective.Outcomes were compared with human condition to validate the model.Macrophages and mast cells were increased. qRT-PCR showed up-regulation of transforming growth factor beta, alpha smooth muscle actin, and rho-associated protein kinase 2 in HSc.

View Article: PubMed Central - PubMed

Affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina.

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Related in: MedlinePlus

Third-degree burn injury model and skin graft survival. (A) Masson's trichrome staining of mouse skin revealed a third-degree burn (e, epidermis; d, dermis; m, muscle; black arrow, third-degree burn). Magnified section shows intact muscular layer. (B) Representative photographs of the dorsal ear skin graft before and after transplantation on the dorsum of the rodent. (C) Top row: donor ears were obtained from C57BL/6 mice and were grafted on GFP transgenic mice. Bottom row: donor ears were obtained from GFP transgenic mice and were grafted onto C57BL/6 mice. Mice were photographed under normal light (left) and the same mice were photographed under GFP excitation light demonstrating graft survival. GFP, green fluorescent protein.
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fig01: Third-degree burn injury model and skin graft survival. (A) Masson's trichrome staining of mouse skin revealed a third-degree burn (e, epidermis; d, dermis; m, muscle; black arrow, third-degree burn). Magnified section shows intact muscular layer. (B) Representative photographs of the dorsal ear skin graft before and after transplantation on the dorsum of the rodent. (C) Top row: donor ears were obtained from C57BL/6 mice and were grafted on GFP transgenic mice. Bottom row: donor ears were obtained from GFP transgenic mice and were grafted onto C57BL/6 mice. Mice were photographed under normal light (left) and the same mice were photographed under GFP excitation light demonstrating graft survival. GFP, green fluorescent protein.

Mentions: A third-degree burn injures the full thickness of the skin: the epidermis and the dermis.19 Histological analysis revealed that a 1-second burn contact time with a 65 g brass rod heated to 100 °C, held in place by gravity, resulted in a reproducible full-thickness burn (Figure 1A). As is noted in the figure, there was necrosis throughout the epidermis and dermis, but not into the deep muscular layer.


A novel immune competent murine hypertrophic scar contracture model: a tool to elucidate disease mechanism and develop new therapies.

Ibrahim MM, Bond J, Bergeron A, Miller KJ, Ehanire T, Quiles C, Lorden ER, Medina MA, Fisher M, Klitzman B, Selim MA, Leong KW, Levinson H - Wound Repair Regen (2015)

Third-degree burn injury model and skin graft survival. (A) Masson's trichrome staining of mouse skin revealed a third-degree burn (e, epidermis; d, dermis; m, muscle; black arrow, third-degree burn). Magnified section shows intact muscular layer. (B) Representative photographs of the dorsal ear skin graft before and after transplantation on the dorsum of the rodent. (C) Top row: donor ears were obtained from C57BL/6 mice and were grafted on GFP transgenic mice. Bottom row: donor ears were obtained from GFP transgenic mice and were grafted onto C57BL/6 mice. Mice were photographed under normal light (left) and the same mice were photographed under GFP excitation light demonstrating graft survival. GFP, green fluorescent protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4304906&req=5

fig01: Third-degree burn injury model and skin graft survival. (A) Masson's trichrome staining of mouse skin revealed a third-degree burn (e, epidermis; d, dermis; m, muscle; black arrow, third-degree burn). Magnified section shows intact muscular layer. (B) Representative photographs of the dorsal ear skin graft before and after transplantation on the dorsum of the rodent. (C) Top row: donor ears were obtained from C57BL/6 mice and were grafted on GFP transgenic mice. Bottom row: donor ears were obtained from GFP transgenic mice and were grafted onto C57BL/6 mice. Mice were photographed under normal light (left) and the same mice were photographed under GFP excitation light demonstrating graft survival. GFP, green fluorescent protein.
Mentions: A third-degree burn injures the full thickness of the skin: the epidermis and the dermis.19 Histological analysis revealed that a 1-second burn contact time with a 65 g brass rod heated to 100 °C, held in place by gravity, resulted in a reproducible full-thickness burn (Figure 1A). As is noted in the figure, there was necrosis throughout the epidermis and dermis, but not into the deep muscular layer.

Bottom Line: Current therapies are marginally effective.Outcomes were compared with human condition to validate the model.Macrophages and mast cells were increased. qRT-PCR showed up-regulation of transforming growth factor beta, alpha smooth muscle actin, and rho-associated protein kinase 2 in HSc.

View Article: PubMed Central - PubMed

Affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina.

Show MeSH
Related in: MedlinePlus