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Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma.

Miyata K, Yotsumoto F, Nam SO, Odawara T, Manabe S, Ishikawa T, Itamochi H, Kigawa J, Takada S, Asahara H, Kuroki M, Miyamoto S - Cancer Med (2014)

Bottom Line: HB-EGF was highly expressed in OCCC cells, and an increase of HB-EGF was induced by SN38 which had only antitumor effect among conventional anticancer agents on OCCC.An in silico and chromatin immunoprecipitation analysis estimated that SP1 bound to the cis-regulatory region of HB-EGF in OCCC cells.Taken together, these results indicate that induction of HB-EGF expression contributed to defense mechanism against treatment of SN38 through the transcriptional activity of SP1 in OCCC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Department of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan; Department of Systems BioMedicine, National Research Institute for Child Health and Development, Tokyo, Japan.

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The expression of HB-EGF in 11 ovarian clear cell carcinoma (OCCC) cell lines. The real-time PCR data show the expression of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AREG) in OCCC cells. Each value represents the mean (n = 3) and standard deviation (SD) of the mRNA expression index for HB-EGF (diagonal striped bars) and AREG (open bars).
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fig01: The expression of HB-EGF in 11 ovarian clear cell carcinoma (OCCC) cell lines. The real-time PCR data show the expression of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AREG) in OCCC cells. Each value represents the mean (n = 3) and standard deviation (SD) of the mRNA expression index for HB-EGF (diagonal striped bars) and AREG (open bars).

Mentions: First, we examined the expression of HB-EGF and AREG in 11 cell lines of OCCC. HB-EGF was highly expressed in all of the cell lines, and eight of the 11 cell lines had a high-expression level of AREG (Fig.1). OVTOKO and ES-2 cells had the highest expression of HB-EGF, while the OVISE and RMG-II cells had higher expression of AREG compared to that of HB-EGF.


Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma.

Miyata K, Yotsumoto F, Nam SO, Odawara T, Manabe S, Ishikawa T, Itamochi H, Kigawa J, Takada S, Asahara H, Kuroki M, Miyamoto S - Cancer Med (2014)

The expression of HB-EGF in 11 ovarian clear cell carcinoma (OCCC) cell lines. The real-time PCR data show the expression of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AREG) in OCCC cells. Each value represents the mean (n = 3) and standard deviation (SD) of the mRNA expression index for HB-EGF (diagonal striped bars) and AREG (open bars).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302667&req=5

fig01: The expression of HB-EGF in 11 ovarian clear cell carcinoma (OCCC) cell lines. The real-time PCR data show the expression of heparin-binding epidermal growth factor-like growth factor (HB-EGF) and amphiregulin (AREG) in OCCC cells. Each value represents the mean (n = 3) and standard deviation (SD) of the mRNA expression index for HB-EGF (diagonal striped bars) and AREG (open bars).
Mentions: First, we examined the expression of HB-EGF and AREG in 11 cell lines of OCCC. HB-EGF was highly expressed in all of the cell lines, and eight of the 11 cell lines had a high-expression level of AREG (Fig.1). OVTOKO and ES-2 cells had the highest expression of HB-EGF, while the OVISE and RMG-II cells had higher expression of AREG compared to that of HB-EGF.

Bottom Line: HB-EGF was highly expressed in OCCC cells, and an increase of HB-EGF was induced by SN38 which had only antitumor effect among conventional anticancer agents on OCCC.An in silico and chromatin immunoprecipitation analysis estimated that SP1 bound to the cis-regulatory region of HB-EGF in OCCC cells.Taken together, these results indicate that induction of HB-EGF expression contributed to defense mechanism against treatment of SN38 through the transcriptional activity of SP1 in OCCC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Department of Biochemistry, Faculty of Medicine, Fukuoka University, Fukuoka, Japan; Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan; Department of Systems BioMedicine, National Research Institute for Child Health and Development, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus