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Decreased expression of KGF/FGF7 and its receptor in pathological hypopigmentation.

Purpura V, Persechino F, Belleudi F, Scrofani C, Raffa S, Persechino S, Torrisi MR - J. Cell. Mol. Med. (2014)

View Article: PubMed Central - PubMed

Affiliation: Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Medicina Clinica e Molecolare, Sapienza Universita' di Roma, Roma, Italy.

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To the Editor: The molecular mechanisms and cellular pathways involved in cutaneous pigmentation, as well as the crucial role played by the epidermal keratinocytes in the process, are just starting to be fibroblasts are known to participate in this complex cellular interplay controlling pigmentation investigated here the efficiency of melanosome transfer in the above-mentioned hypopigmentation In addition, ELISA test demonstrated that KGF protein levels were significantly decreased in SNs from all lesional HFs compared with NHFs (Fig. 1C)... Interestingly, consistent with the mRNA expression data, the KGF released by vitiligo HFs was significantly reduced if compared with that secreted by both ND HFs and rSutton HFs (Fig... the KGF-induced increase of the tyrosinase-positive dots in the cytoplasm of ND HKs was much lower compared with NHKs (Fig. 2A, middle panels)... Therefore, with the aim to analyse the receptor expression, we quantified KGFR transcript levels by real-time RT-PCR and we found a decreased receptor mRNA expression in ND Thus, at least in the ND disorder, low levels of KGFR might significantly contribute to the reduction of normal skin, by KGF/FGF7 released by dermal fibroblasts and by its receptor KGFR/FGFR2b expressed

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Decreased melanosome uptake ability and KGFR expression in keratinocytes from ND lesion.(A and B) Cocultures of MST-L melanoma cells with normal humankeratinocytes (NHKs) or with keratinocytes derived from the ND lesion (ND HKs) were treated withKGF. Immunofluorescence (A and B) and phase-contrast (B)images show that the tyrosinase-positive dots in ND HKs upon KGF treatment are strongly reduced withrespect to those in NHKs (A and B, circles) and that the addition ofSU5402 abolishes the KGF effect; bars: 10 μm. (C) Real-time RT-PCR reveals adecreased KGFR mRNA expression in ND HKs compared with NHK control cells. (D) Schematicdrawing showing the effects of decreased levels of KGF and KGFR on melanosome transfer inhypopigmented lesions.
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fig02: Decreased melanosome uptake ability and KGFR expression in keratinocytes from ND lesion.(A and B) Cocultures of MST-L melanoma cells with normal humankeratinocytes (NHKs) or with keratinocytes derived from the ND lesion (ND HKs) were treated withKGF. Immunofluorescence (A and B) and phase-contrast (B)images show that the tyrosinase-positive dots in ND HKs upon KGF treatment are strongly reduced withrespect to those in NHKs (A and B, circles) and that the addition ofSU5402 abolishes the KGF effect; bars: 10 μm. (C) Real-time RT-PCR reveals adecreased KGFR mRNA expression in ND HKs compared with NHK control cells. (D) Schematicdrawing showing the effects of decreased levels of KGF and KGFR on melanosome transfer inhypopigmented lesions.

Mentions: To evaluate the contribution of the lesional keratinocytes on the inefficient melanosometransfer, we focused our attention on the above ND biopsy, because of the postulated defect of theorganelle uptake in such disorder [11,12]. To dissect in vitro the process, wecocultured the MST-L melanocytes with primary keratinocytes derived from the ND (ND HKs) or fromnormal skin, at a seeding ratio of 1:40. Serum starvation and treatment with KGF in the presence orabsence of SU5402 were performed as above. The quantitative double immunofluorescence revealed thatthe KGF-induced increase of the tyrosinase-positive dots in the cytoplasm of ND HKs was much lowercompared with NHKs (Fig. 2A, middle panels). Brightfield andphase-contrast microscopy were used to unequivocally demonstrate the decreased melanosome transferto the lesional keratinocytes (Fig. 2B). Again, the additionof SU5402 was able to abolish the KGF effect in both cocultures (Fig. 2A, lower panels), providing a further evidence of the involvement of KGFRactivation and signalling in the process and suggesting a decreased receptor expression in thepathological condition. Therefore, with the aim to analyse the receptor expression, we quantifiedKGFR transcript levels by real-time RT-PCR and we found a decreased receptor mRNA expression in NDHKs compared with NHK control cells (Fig. 2C). Thus, atleast in the ND disorder, low levels of KGFR might significantly contribute to the reduction ofKGF-mediated melanosome transfer.


Decreased expression of KGF/FGF7 and its receptor in pathological hypopigmentation.

Purpura V, Persechino F, Belleudi F, Scrofani C, Raffa S, Persechino S, Torrisi MR - J. Cell. Mol. Med. (2014)

Decreased melanosome uptake ability and KGFR expression in keratinocytes from ND lesion.(A and B) Cocultures of MST-L melanoma cells with normal humankeratinocytes (NHKs) or with keratinocytes derived from the ND lesion (ND HKs) were treated withKGF. Immunofluorescence (A and B) and phase-contrast (B)images show that the tyrosinase-positive dots in ND HKs upon KGF treatment are strongly reduced withrespect to those in NHKs (A and B, circles) and that the addition ofSU5402 abolishes the KGF effect; bars: 10 μm. (C) Real-time RT-PCR reveals adecreased KGFR mRNA expression in ND HKs compared with NHK control cells. (D) Schematicdrawing showing the effects of decreased levels of KGF and KGFR on melanosome transfer inhypopigmented lesions.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4302659&req=5

fig02: Decreased melanosome uptake ability and KGFR expression in keratinocytes from ND lesion.(A and B) Cocultures of MST-L melanoma cells with normal humankeratinocytes (NHKs) or with keratinocytes derived from the ND lesion (ND HKs) were treated withKGF. Immunofluorescence (A and B) and phase-contrast (B)images show that the tyrosinase-positive dots in ND HKs upon KGF treatment are strongly reduced withrespect to those in NHKs (A and B, circles) and that the addition ofSU5402 abolishes the KGF effect; bars: 10 μm. (C) Real-time RT-PCR reveals adecreased KGFR mRNA expression in ND HKs compared with NHK control cells. (D) Schematicdrawing showing the effects of decreased levels of KGF and KGFR on melanosome transfer inhypopigmented lesions.
Mentions: To evaluate the contribution of the lesional keratinocytes on the inefficient melanosometransfer, we focused our attention on the above ND biopsy, because of the postulated defect of theorganelle uptake in such disorder [11,12]. To dissect in vitro the process, wecocultured the MST-L melanocytes with primary keratinocytes derived from the ND (ND HKs) or fromnormal skin, at a seeding ratio of 1:40. Serum starvation and treatment with KGF in the presence orabsence of SU5402 were performed as above. The quantitative double immunofluorescence revealed thatthe KGF-induced increase of the tyrosinase-positive dots in the cytoplasm of ND HKs was much lowercompared with NHKs (Fig. 2A, middle panels). Brightfield andphase-contrast microscopy were used to unequivocally demonstrate the decreased melanosome transferto the lesional keratinocytes (Fig. 2B). Again, the additionof SU5402 was able to abolish the KGF effect in both cocultures (Fig. 2A, lower panels), providing a further evidence of the involvement of KGFRactivation and signalling in the process and suggesting a decreased receptor expression in thepathological condition. Therefore, with the aim to analyse the receptor expression, we quantifiedKGFR transcript levels by real-time RT-PCR and we found a decreased receptor mRNA expression in NDHKs compared with NHK control cells (Fig. 2C). Thus, atleast in the ND disorder, low levels of KGFR might significantly contribute to the reduction ofKGF-mediated melanosome transfer.

View Article: PubMed Central - PubMed

Affiliation: Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Medicina Clinica e Molecolare, Sapienza Universita' di Roma, Roma, Italy.

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

To the Editor: The molecular mechanisms and cellular pathways involved in cutaneous pigmentation, as well as the crucial role played by the epidermal keratinocytes in the process, are just starting to be fibroblasts are known to participate in this complex cellular interplay controlling pigmentation investigated here the efficiency of melanosome transfer in the above-mentioned hypopigmentation In addition, ELISA test demonstrated that KGF protein levels were significantly decreased in SNs from all lesional HFs compared with NHFs (Fig. 1C)... Interestingly, consistent with the mRNA expression data, the KGF released by vitiligo HFs was significantly reduced if compared with that secreted by both ND HFs and rSutton HFs (Fig... the KGF-induced increase of the tyrosinase-positive dots in the cytoplasm of ND HKs was much lower compared with NHKs (Fig. 2A, middle panels)... Therefore, with the aim to analyse the receptor expression, we quantified KGFR transcript levels by real-time RT-PCR and we found a decreased receptor mRNA expression in ND Thus, at least in the ND disorder, low levels of KGFR might significantly contribute to the reduction of normal skin, by KGF/FGF7 released by dermal fibroblasts and by its receptor KGFR/FGFR2b expressed

Show MeSH
Related in: MedlinePlus