Eye-mediated immune tolerance to Type II collagen in arthritis-prone strains of mice.
Bottom Line: Type II collagen (CII) is a cartilage structural protein that plays important roles in joint function, arthritis and ageing.Specific immune tolerance induction was assessed using both delayed-type hypersensitivity (DTH) and local adoptive transfer (LAT) assays.These findings could be beneficial in studies of immune tolerance induction using CII.
Affiliation: Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA.Show MeSH
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Mentions: We adoptively transferred ACAID APCs into naïve C57BL6 and DBA/1 mice on day 0 to examine the hypothesis that in vitro-generated CII-specific ACAID APCs induce peripheral tolerance in C57BL6 and DBA/1 mice. The protocol for generating ACAID APCs is described in the methods section. On day 7, the recipient mice were subcutaneously immunized with CII emulsified with CFA. These mice were challenged with CII via intradermal injections in the ear on day 14. The significant suppression of DTH responses observed in these mice as compared with the positive control mice that did not receive ACAID APCs shows that tail vein injection of CII-specific ACAID APCs induced immune tolerance in both arthritis-prone strains of mice (Fig. 2A and B). The statistical significance data of experiments on C57BL/6 and DBA/1 mice was as follows: Figure 2A (C57BL/6): ACAID mice versus positive control, *P = 0.004 (24 h); *P = 0.0025 (48 h); Figure 2B (DBA/1): ACAID mice versus positive control, *P = 0.000012 (24 h); *P = 0.00029 (48 h).
Affiliation: Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, USA.