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Strain and stress variations in the human amniotic membrane and fresh corpse autologous sciatic nerve anastomosis in a model of sciatic nerve injury.

Peng C, Zhang Q, Yang Q, Zhu Q - Neural Regen Res (2012)

Bottom Line: Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens.Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group.The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130029, Jilin Province, China.

ABSTRACT
A 10-mm long sciatic nerve injury model was established in fresh normal Chinese patient cadavers. Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens. Sciatic nerve injury models were respectively anastomosed using the autologous cadaveric sciatic nerve and human amniotic membrane. Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group. The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity. Experimental findings suggested that human amniotic membranes and autologous sciatic nerves exhibit similar stress-strain curves, good elastic properties, and certain strain and stress capabilities in anastomosis of the injured sciatic nerve.

No MeSH data available.


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Morphology of sciatic nerve cross-sections in normal sciatic nerve group (A) and autologous nerve anastomosis group (B) in tensile test (scanning electron microscopy, × 2 000).
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Figure 3: Morphology of sciatic nerve cross-sections in normal sciatic nerve group (A) and autologous nerve anastomosis group (B) in tensile test (scanning electron microscopy, × 2 000).

Mentions: Scanning electron microscopy showed that normal sciatic nerve fibers were orderly arranged, axons were surrounded by a myelin sheath, and the endoneurium-encased myelin sheath and axons were clear and visible on the nerve fiber surface (Figure 3A). Following anastomosis with autologous nerve anastomosis, the injured sciatic nerve fibers were disorderly arranged, and the surface connective tissue, endoneurium, myelin sheath, and axons morphology changed and even ruptured, thus blocking the basilar membrane cavity (Figure 3B).


Strain and stress variations in the human amniotic membrane and fresh corpse autologous sciatic nerve anastomosis in a model of sciatic nerve injury.

Peng C, Zhang Q, Yang Q, Zhu Q - Neural Regen Res (2012)

Morphology of sciatic nerve cross-sections in normal sciatic nerve group (A) and autologous nerve anastomosis group (B) in tensile test (scanning electron microscopy, × 2 000).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302526&req=5

Figure 3: Morphology of sciatic nerve cross-sections in normal sciatic nerve group (A) and autologous nerve anastomosis group (B) in tensile test (scanning electron microscopy, × 2 000).
Mentions: Scanning electron microscopy showed that normal sciatic nerve fibers were orderly arranged, axons were surrounded by a myelin sheath, and the endoneurium-encased myelin sheath and axons were clear and visible on the nerve fiber surface (Figure 3A). Following anastomosis with autologous nerve anastomosis, the injured sciatic nerve fibers were disorderly arranged, and the surface connective tissue, endoneurium, myelin sheath, and axons morphology changed and even ruptured, thus blocking the basilar membrane cavity (Figure 3B).

Bottom Line: Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens.Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group.The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130029, Jilin Province, China.

ABSTRACT
A 10-mm long sciatic nerve injury model was established in fresh normal Chinese patient cadavers. Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens. Sciatic nerve injury models were respectively anastomosed using the autologous cadaveric sciatic nerve and human amniotic membrane. Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group. The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity. Experimental findings suggested that human amniotic membranes and autologous sciatic nerves exhibit similar stress-strain curves, good elastic properties, and certain strain and stress capabilities in anastomosis of the injured sciatic nerve.

No MeSH data available.


Related in: MedlinePlus