Limits...
Strain and stress variations in the human amniotic membrane and fresh corpse autologous sciatic nerve anastomosis in a model of sciatic nerve injury.

Peng C, Zhang Q, Yang Q, Zhu Q - Neural Regen Res (2012)

Bottom Line: Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens.Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group.The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130029, Jilin Province, China.

ABSTRACT
A 10-mm long sciatic nerve injury model was established in fresh normal Chinese patient cadavers. Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens. Sciatic nerve injury models were respectively anastomosed using the autologous cadaveric sciatic nerve and human amniotic membrane. Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group. The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity. Experimental findings suggested that human amniotic membranes and autologous sciatic nerves exhibit similar stress-strain curves, good elastic properties, and certain strain and stress capabilities in anastomosis of the injured sciatic nerve.

No MeSH data available.


Related in: MedlinePlus

Tensile stress-strain curve of normal human amnion and amniotic membrane anastomosis on injured sciatic nerve (Y axis represents strain, X axis represents stress).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4302526&req=5

Figure 2: Tensile stress-strain curve of normal human amnion and amniotic membrane anastomosis on injured sciatic nerve (Y axis represents strain, X axis represents stress).

Mentions: The tensile specimen data from each group was subjected to curve fitting on computers. The stress-strain curves of the normal sciatic nerve group and autologous nerve anastomosis group are shown in Figure 1. Results showed that the curve exponentially increased at the first phase, and the strain in both groups was 0–5%, respectively. At the second phase, the curve was directly proportional to elastic deformations; strain was 5.1–20.0% in the normal sciatic nerve group and 5.1–10.0% in the autologous nerve anastomosis group. At the third phase, the stress-strain curve, similar to the first phase, exhibited elastic deformations; strain increased to 20.1–28.0% in the normal sciatic nerve group and 10.1–17.8% in the autologous nerve anastomosis group. However, specimens exhibited deformations and lost bearing capacities at the fourth phase; strain was 28.1–30.3% in the normal sciatic nerve group and 17.8–21.9% in the autologous nerve anastomosis group. Alterations in the stress-strain curve of the normal sciatic nerve group and autologous nerve anastomosis group indicated good elasticity in the sciatic nerve. The stress-strain curves of the normal human amnion group and human amniotic membrane anastomosis group are shown in Figure 2. Results showed that the curve exponentially changed at the first phase in both groups, and strain was 0–2%, respectively. At the second phase, the curve was directly proportional to elastic deformations; strain was 2.1–8.0% in the normal human amnion group and 2.1–6.0% in the human amniotic membrane anastomosis group. At the third phase, the stress-strain curve, which was similar to the first phase, exhibited elastic deformations; strain increased to 8.1–14.0% in the normal human amnion group and 6.1–10.0% in the human amniotic membrane anastomosis group. However, specimens exhibited deformations and lost bearing capacities at the fourth phase; strain was 14.1–16.9% in the normal human amnion group and 10.1–11.5% in the human amniotic membrane anastomosis group. Changes in the stress- strain curves of the normal human amnion group and human amniotic membrane anastomosis group indicated good elasticity and plasticity in the human amnion.


Strain and stress variations in the human amniotic membrane and fresh corpse autologous sciatic nerve anastomosis in a model of sciatic nerve injury.

Peng C, Zhang Q, Yang Q, Zhu Q - Neural Regen Res (2012)

Tensile stress-strain curve of normal human amnion and amniotic membrane anastomosis on injured sciatic nerve (Y axis represents strain, X axis represents stress).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302526&req=5

Figure 2: Tensile stress-strain curve of normal human amnion and amniotic membrane anastomosis on injured sciatic nerve (Y axis represents strain, X axis represents stress).
Mentions: The tensile specimen data from each group was subjected to curve fitting on computers. The stress-strain curves of the normal sciatic nerve group and autologous nerve anastomosis group are shown in Figure 1. Results showed that the curve exponentially increased at the first phase, and the strain in both groups was 0–5%, respectively. At the second phase, the curve was directly proportional to elastic deformations; strain was 5.1–20.0% in the normal sciatic nerve group and 5.1–10.0% in the autologous nerve anastomosis group. At the third phase, the stress-strain curve, similar to the first phase, exhibited elastic deformations; strain increased to 20.1–28.0% in the normal sciatic nerve group and 10.1–17.8% in the autologous nerve anastomosis group. However, specimens exhibited deformations and lost bearing capacities at the fourth phase; strain was 28.1–30.3% in the normal sciatic nerve group and 17.8–21.9% in the autologous nerve anastomosis group. Alterations in the stress-strain curve of the normal sciatic nerve group and autologous nerve anastomosis group indicated good elasticity in the sciatic nerve. The stress-strain curves of the normal human amnion group and human amniotic membrane anastomosis group are shown in Figure 2. Results showed that the curve exponentially changed at the first phase in both groups, and strain was 0–2%, respectively. At the second phase, the curve was directly proportional to elastic deformations; strain was 2.1–8.0% in the normal human amnion group and 2.1–6.0% in the human amniotic membrane anastomosis group. At the third phase, the stress-strain curve, which was similar to the first phase, exhibited elastic deformations; strain increased to 8.1–14.0% in the normal human amnion group and 6.1–10.0% in the human amniotic membrane anastomosis group. However, specimens exhibited deformations and lost bearing capacities at the fourth phase; strain was 14.1–16.9% in the normal human amnion group and 10.1–11.5% in the human amniotic membrane anastomosis group. Changes in the stress- strain curves of the normal human amnion group and human amniotic membrane anastomosis group indicated good elasticity and plasticity in the human amnion.

Bottom Line: Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens.Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group.The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130029, Jilin Province, China.

ABSTRACT
A 10-mm long sciatic nerve injury model was established in fresh normal Chinese patient cadavers. Amniotic membrane was harvested from healthy maternal placentas and was prepared into multilayered, coiled, tubular specimens. Sciatic nerve injury models were respectively anastomosed using the autologous cadaveric sciatic nerve and human amniotic membrane. Tensile test results showed that maximal loading, maximal displacement, maximal stress, and maximal strain of sciatic nerve injury models anastomosed with human amniotic membrane were greater than those in the autologous nerve anastomosis group. The strain-stress curves of the human amniotic membrane and sciatic nerves indicated exponential change at the first phase, which became elastic deformation curves at the second and third phases, and displayed plastic deformation curves at the fourth phase, at which point the specimens lost their bearing capacity. Experimental findings suggested that human amniotic membranes and autologous sciatic nerves exhibit similar stress-strain curves, good elastic properties, and certain strain and stress capabilities in anastomosis of the injured sciatic nerve.

No MeSH data available.


Related in: MedlinePlus