Limits...
Transcriptome analysis and transient transformation suggest an ancient duplicated MYB transcription factor as a candidate gene for leaf red coloration in peach.

Zhou Y, Zhou H, Lin-Wang K, Vimolmangkang S, Espley RV, Wang L, Allan AC, Han Y - BMC Plant Biol. (2014)

Bottom Line: This suggests a complex mechanism underlying anthocyanin accumulation in peach leaf.However, PpMYB10.4 has diverged from these anthocyanin-activating MYBs to generate a new gene family, which regulates anthocyanin accumulation in vegetative organs such as leaves.Activation of an ancient duplicated MYB gene PpMYB10.4 in the Gr interval on LG 6, which represents a novel branch of anthocyanin-activating MYB genes in Rosaceae, is able to activate leaf red coloration in peach.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Leaf red coloration is an important characteristic in many plant species, including cultivars of ornamental peach (Prunus persica). Peach leaf color is controlled by a single Gr gene on linkage group 6, with a red allele dominant over the green allele. Here, we report the identification of a candidate gene of Gr in peach.

Results: The red coloration of peach leaves is due to accumulation of anthocyanin pigments, which is regulated at the transcriptional level. Based on transcriptome comparison between red- and green-colored leaves, an MYB transcription regulator PpMYB10.4 in the Gr interval was identified to regulate anthocyanin pigmentation in peach leaf. Transient expression of PpMYB10.4 in tobacco and peach leaves can induce anthocyain accumulation. Moreover, a functional MYB gene PpMYB10.2 on linkage group 3, which is homologous to PpMYB10.4, is also expressed in both red- and green-colored leaves, but plays no role in leaf red coloration. This suggests a complex mechanism underlying anthocyanin accumulation in peach leaf. In addition, PpMYB10.4 and other anthocyanin-activating MYB genes in Rosaceae responsible for anthocyanin accumulation in fruit are dated to a common ancestor about 70 million years ago (MYA). However, PpMYB10.4 has diverged from these anthocyanin-activating MYBs to generate a new gene family, which regulates anthocyanin accumulation in vegetative organs such as leaves.

Conclusions: Activation of an ancient duplicated MYB gene PpMYB10.4 in the Gr interval on LG 6, which represents a novel branch of anthocyanin-activating MYB genes in Rosaceae, is able to activate leaf red coloration in peach.

Show MeSH
Six anthocyanin-related MYB genes in the peach genome. A, Structural feature and chromosomal position of the six peach MYB genes. B, Estimated divergence time between anthocyanin-related MYB genes in plants based on aligned nucleotide sequences using Bayesian MCMC analysis. The GenBank accession numbers are as follows: Prunus domestica PdMYB10 (ABX71492); Malus × domestica MdMYB10 (AFC88038), MdMYB110a (JN711473), and MdMYB110b (JN711474); Pyrus communis PyMYB10 (JX403957); Cydonia oblonga CoMYB10 (EU153571); Citrus sinensis CsRuby (AFB73909); Vitis vinifera VvMYB1a (ABB87014); Ipomoea batatas IbMYB1 (BAF45114); Arabidopsis AtPAP1 (NP_176057), AtPAP2 (NP_176813), AtMYB11 (NP_191820), and AtMYB113 (NP_176811); Antirrhinum majus AmRosea1 (ABB83826), Zea mays ZmC1 (NM_001112540); and Oryza sativa OsC1 (HQ379703). Pm001924 and MDP0000573302 are extracted from the released genome sequences of Prunus mume [27] and apple, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4302523&req=5

Fig1: Six anthocyanin-related MYB genes in the peach genome. A, Structural feature and chromosomal position of the six peach MYB genes. B, Estimated divergence time between anthocyanin-related MYB genes in plants based on aligned nucleotide sequences using Bayesian MCMC analysis. The GenBank accession numbers are as follows: Prunus domestica PdMYB10 (ABX71492); Malus × domestica MdMYB10 (AFC88038), MdMYB110a (JN711473), and MdMYB110b (JN711474); Pyrus communis PyMYB10 (JX403957); Cydonia oblonga CoMYB10 (EU153571); Citrus sinensis CsRuby (AFB73909); Vitis vinifera VvMYB1a (ABB87014); Ipomoea batatas IbMYB1 (BAF45114); Arabidopsis AtPAP1 (NP_176057), AtPAP2 (NP_176813), AtMYB11 (NP_191820), and AtMYB113 (NP_176811); Antirrhinum majus AmRosea1 (ABB83826), Zea mays ZmC1 (NM_001112540); and Oryza sativa OsC1 (HQ379703). Pm001924 and MDP0000573302 are extracted from the released genome sequences of Prunus mume [27] and apple, respectively.

Mentions: To determinate whether multiple MYB genes are involved in the regulation of anthocyanin accumulation in peach leaves, we compared the cDNA sequences of PpMYB10.2 and PpMYB10.4 against the draft genome of peach cv. Lovell using blastn [1]. As a result, PpMYB10.2 and its two paralogs, termed PpMYB10.1 and PpMYB10.3 [20], were located next to each other within a 72 kb region on chromosome 3, while PpMYB10.4 and its two paralogs (PpMYB10.5 and PpMYB10.6) were clustered within a 63 kb region on chromosome 6 (Figure 1A). Accession numbers of PpMYB10.1 to PpMYB10.6 at the Genome Database for Rosaceae (GDR, http://www.rosaceae.org/) were listed in Additional file 1: Table S1. PpMYB10.2 was identical in sequence to PpMYB10 previously isolated from peach fruit [13]. PpMYB10.3 and PpMYB10.1 have recently been implicated in peach fruit pigmentation [20]. All the six MYB TFs consist of three exons separated by two introns. The consensus sequences, GC and AG, were found at the 5′ and 3′-borders of the two introns of PpMYB10.1 to PpMYB10.3, strictly following the “GT–AG” splicing site of the eukaryotic introns proposed by Breathnach and Chambon [24]. In contrast, “GT–AG” and “GC-AG” splicing sites were observed for the first and second introns of PpMYB10.4 to PpMYB10.6, respectively.Figure 1


Transcriptome analysis and transient transformation suggest an ancient duplicated MYB transcription factor as a candidate gene for leaf red coloration in peach.

Zhou Y, Zhou H, Lin-Wang K, Vimolmangkang S, Espley RV, Wang L, Allan AC, Han Y - BMC Plant Biol. (2014)

Six anthocyanin-related MYB genes in the peach genome. A, Structural feature and chromosomal position of the six peach MYB genes. B, Estimated divergence time between anthocyanin-related MYB genes in plants based on aligned nucleotide sequences using Bayesian MCMC analysis. The GenBank accession numbers are as follows: Prunus domestica PdMYB10 (ABX71492); Malus × domestica MdMYB10 (AFC88038), MdMYB110a (JN711473), and MdMYB110b (JN711474); Pyrus communis PyMYB10 (JX403957); Cydonia oblonga CoMYB10 (EU153571); Citrus sinensis CsRuby (AFB73909); Vitis vinifera VvMYB1a (ABB87014); Ipomoea batatas IbMYB1 (BAF45114); Arabidopsis AtPAP1 (NP_176057), AtPAP2 (NP_176813), AtMYB11 (NP_191820), and AtMYB113 (NP_176811); Antirrhinum majus AmRosea1 (ABB83826), Zea mays ZmC1 (NM_001112540); and Oryza sativa OsC1 (HQ379703). Pm001924 and MDP0000573302 are extracted from the released genome sequences of Prunus mume [27] and apple, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4302523&req=5

Fig1: Six anthocyanin-related MYB genes in the peach genome. A, Structural feature and chromosomal position of the six peach MYB genes. B, Estimated divergence time between anthocyanin-related MYB genes in plants based on aligned nucleotide sequences using Bayesian MCMC analysis. The GenBank accession numbers are as follows: Prunus domestica PdMYB10 (ABX71492); Malus × domestica MdMYB10 (AFC88038), MdMYB110a (JN711473), and MdMYB110b (JN711474); Pyrus communis PyMYB10 (JX403957); Cydonia oblonga CoMYB10 (EU153571); Citrus sinensis CsRuby (AFB73909); Vitis vinifera VvMYB1a (ABB87014); Ipomoea batatas IbMYB1 (BAF45114); Arabidopsis AtPAP1 (NP_176057), AtPAP2 (NP_176813), AtMYB11 (NP_191820), and AtMYB113 (NP_176811); Antirrhinum majus AmRosea1 (ABB83826), Zea mays ZmC1 (NM_001112540); and Oryza sativa OsC1 (HQ379703). Pm001924 and MDP0000573302 are extracted from the released genome sequences of Prunus mume [27] and apple, respectively.
Mentions: To determinate whether multiple MYB genes are involved in the regulation of anthocyanin accumulation in peach leaves, we compared the cDNA sequences of PpMYB10.2 and PpMYB10.4 against the draft genome of peach cv. Lovell using blastn [1]. As a result, PpMYB10.2 and its two paralogs, termed PpMYB10.1 and PpMYB10.3 [20], were located next to each other within a 72 kb region on chromosome 3, while PpMYB10.4 and its two paralogs (PpMYB10.5 and PpMYB10.6) were clustered within a 63 kb region on chromosome 6 (Figure 1A). Accession numbers of PpMYB10.1 to PpMYB10.6 at the Genome Database for Rosaceae (GDR, http://www.rosaceae.org/) were listed in Additional file 1: Table S1. PpMYB10.2 was identical in sequence to PpMYB10 previously isolated from peach fruit [13]. PpMYB10.3 and PpMYB10.1 have recently been implicated in peach fruit pigmentation [20]. All the six MYB TFs consist of three exons separated by two introns. The consensus sequences, GC and AG, were found at the 5′ and 3′-borders of the two introns of PpMYB10.1 to PpMYB10.3, strictly following the “GT–AG” splicing site of the eukaryotic introns proposed by Breathnach and Chambon [24]. In contrast, “GT–AG” and “GC-AG” splicing sites were observed for the first and second introns of PpMYB10.4 to PpMYB10.6, respectively.Figure 1

Bottom Line: This suggests a complex mechanism underlying anthocyanin accumulation in peach leaf.However, PpMYB10.4 has diverged from these anthocyanin-activating MYBs to generate a new gene family, which regulates anthocyanin accumulation in vegetative organs such as leaves.Activation of an ancient duplicated MYB gene PpMYB10.4 in the Gr interval on LG 6, which represents a novel branch of anthocyanin-activating MYB genes in Rosaceae, is able to activate leaf red coloration in peach.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Leaf red coloration is an important characteristic in many plant species, including cultivars of ornamental peach (Prunus persica). Peach leaf color is controlled by a single Gr gene on linkage group 6, with a red allele dominant over the green allele. Here, we report the identification of a candidate gene of Gr in peach.

Results: The red coloration of peach leaves is due to accumulation of anthocyanin pigments, which is regulated at the transcriptional level. Based on transcriptome comparison between red- and green-colored leaves, an MYB transcription regulator PpMYB10.4 in the Gr interval was identified to regulate anthocyanin pigmentation in peach leaf. Transient expression of PpMYB10.4 in tobacco and peach leaves can induce anthocyain accumulation. Moreover, a functional MYB gene PpMYB10.2 on linkage group 3, which is homologous to PpMYB10.4, is also expressed in both red- and green-colored leaves, but plays no role in leaf red coloration. This suggests a complex mechanism underlying anthocyanin accumulation in peach leaf. In addition, PpMYB10.4 and other anthocyanin-activating MYB genes in Rosaceae responsible for anthocyanin accumulation in fruit are dated to a common ancestor about 70 million years ago (MYA). However, PpMYB10.4 has diverged from these anthocyanin-activating MYBs to generate a new gene family, which regulates anthocyanin accumulation in vegetative organs such as leaves.

Conclusions: Activation of an ancient duplicated MYB gene PpMYB10.4 in the Gr interval on LG 6, which represents a novel branch of anthocyanin-activating MYB genes in Rosaceae, is able to activate leaf red coloration in peach.

Show MeSH