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Sonic hedgehog elevates N-myc gene expression in neural stem cells.

Liu D, Wang S, Cui Y, Shen L, Du Y, Li G, Zhang B, Wang R - Neural Regen Res (2012)

Bottom Line: The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gli1 and N-myc, a target gene of sonic hedgehog.These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells.Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.

ABSTRACT
Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gli1 and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

No MeSH data available.


Related in: MedlinePlus

Observation of enhanced green fluorescent protein (EGFP) expression in neural stem cells (NSCs) after rAAV-SHH-EGFP infection (× 100).(A) Primary culture of NSCs after rAAV-SHH-N-EGFP infection for 14 days.(B) The same cells shown in A, observed under inverted fluorescence microscope.rAAV: Recombinant adeno-associated virus; SHH: sonic hedgehog.
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Figure 4: Observation of enhanced green fluorescent protein (EGFP) expression in neural stem cells (NSCs) after rAAV-SHH-EGFP infection (× 100).(A) Primary culture of NSCs after rAAV-SHH-N-EGFP infection for 14 days.(B) The same cells shown in A, observed under inverted fluorescence microscope.rAAV: Recombinant adeno-associated virus; SHH: sonic hedgehog.

Mentions: EGFP, as a reporter protein for SHH-N, was expressed in neural stem cells 14 days after rAAV-SHH-N-EGFP infection Figure 4, supplementary Figure 3 online).


Sonic hedgehog elevates N-myc gene expression in neural stem cells.

Liu D, Wang S, Cui Y, Shen L, Du Y, Li G, Zhang B, Wang R - Neural Regen Res (2012)

Observation of enhanced green fluorescent protein (EGFP) expression in neural stem cells (NSCs) after rAAV-SHH-EGFP infection (× 100).(A) Primary culture of NSCs after rAAV-SHH-N-EGFP infection for 14 days.(B) The same cells shown in A, observed under inverted fluorescence microscope.rAAV: Recombinant adeno-associated virus; SHH: sonic hedgehog.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302450&req=5

Figure 4: Observation of enhanced green fluorescent protein (EGFP) expression in neural stem cells (NSCs) after rAAV-SHH-EGFP infection (× 100).(A) Primary culture of NSCs after rAAV-SHH-N-EGFP infection for 14 days.(B) The same cells shown in A, observed under inverted fluorescence microscope.rAAV: Recombinant adeno-associated virus; SHH: sonic hedgehog.
Mentions: EGFP, as a reporter protein for SHH-N, was expressed in neural stem cells 14 days after rAAV-SHH-N-EGFP infection Figure 4, supplementary Figure 3 online).

Bottom Line: The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gli1 and N-myc, a target gene of sonic hedgehog.These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells.Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.

ABSTRACT
Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gli1 and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

No MeSH data available.


Related in: MedlinePlus