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Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro.

Lu J, Lu K, Li D - Neural Regen Res (2012)

Bottom Line: Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors.Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche.Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China ; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.

ABSTRACT
In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence analysis of FGF8 expressions and distribution during neural stem/progenitor cell differentiation in vitro.(A–C) Immunofluorescence staining on days 3, 10 and 20 during NSC/NPC differentiation. Green (FITC): FGF8+; red (TRITC): Nestin+; multicolor: merged. Scale bars: A, 100 μm; B, 50 μm; C, 200 μm.(D) Relative intensity of FGF8+ (green) and Nestin+ (red) fluorescence signals, and the signal ratios of FGF8 to Nestin (green/red) on days 3, 10 or 20 during NSC/NPC differentiation (mean ± SD, n = 4). Nestin+ fluorescence signal intensity was used as a control.The ratio of FGF8+ fluorescence intensity to nestin+ fluorescence intensity reflected the relative expression level of FGF8 at each stage. aP < 0.05, vs. that of the prior differentiation stage (Duncan multiple comparison test).FGF8: Fibroblast growth factor 8; NSC/NPC: neural stem/progenitor cell.
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Figure 4: Immunofluorescence analysis of FGF8 expressions and distribution during neural stem/progenitor cell differentiation in vitro.(A–C) Immunofluorescence staining on days 3, 10 and 20 during NSC/NPC differentiation. Green (FITC): FGF8+; red (TRITC): Nestin+; multicolor: merged. Scale bars: A, 100 μm; B, 50 μm; C, 200 μm.(D) Relative intensity of FGF8+ (green) and Nestin+ (red) fluorescence signals, and the signal ratios of FGF8 to Nestin (green/red) on days 3, 10 or 20 during NSC/NPC differentiation (mean ± SD, n = 4). Nestin+ fluorescence signal intensity was used as a control.The ratio of FGF8+ fluorescence intensity to nestin+ fluorescence intensity reflected the relative expression level of FGF8 at each stage. aP < 0.05, vs. that of the prior differentiation stage (Duncan multiple comparison test).FGF8: Fibroblast growth factor 8; NSC/NPC: neural stem/progenitor cell.

Mentions: The relative intensity of nestin+ fluorescence staining on neural stem/progenitor cells during differentiation in vitro indicated no significant difference on any of the days tested (days 3, 10 and 20, P > 0.05; Figure 4).


Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro.

Lu J, Lu K, Li D - Neural Regen Res (2012)

Immunofluorescence analysis of FGF8 expressions and distribution during neural stem/progenitor cell differentiation in vitro.(A–C) Immunofluorescence staining on days 3, 10 and 20 during NSC/NPC differentiation. Green (FITC): FGF8+; red (TRITC): Nestin+; multicolor: merged. Scale bars: A, 100 μm; B, 50 μm; C, 200 μm.(D) Relative intensity of FGF8+ (green) and Nestin+ (red) fluorescence signals, and the signal ratios of FGF8 to Nestin (green/red) on days 3, 10 or 20 during NSC/NPC differentiation (mean ± SD, n = 4). Nestin+ fluorescence signal intensity was used as a control.The ratio of FGF8+ fluorescence intensity to nestin+ fluorescence intensity reflected the relative expression level of FGF8 at each stage. aP < 0.05, vs. that of the prior differentiation stage (Duncan multiple comparison test).FGF8: Fibroblast growth factor 8; NSC/NPC: neural stem/progenitor cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302448&req=5

Figure 4: Immunofluorescence analysis of FGF8 expressions and distribution during neural stem/progenitor cell differentiation in vitro.(A–C) Immunofluorescence staining on days 3, 10 and 20 during NSC/NPC differentiation. Green (FITC): FGF8+; red (TRITC): Nestin+; multicolor: merged. Scale bars: A, 100 μm; B, 50 μm; C, 200 μm.(D) Relative intensity of FGF8+ (green) and Nestin+ (red) fluorescence signals, and the signal ratios of FGF8 to Nestin (green/red) on days 3, 10 or 20 during NSC/NPC differentiation (mean ± SD, n = 4). Nestin+ fluorescence signal intensity was used as a control.The ratio of FGF8+ fluorescence intensity to nestin+ fluorescence intensity reflected the relative expression level of FGF8 at each stage. aP < 0.05, vs. that of the prior differentiation stage (Duncan multiple comparison test).FGF8: Fibroblast growth factor 8; NSC/NPC: neural stem/progenitor cell.
Mentions: The relative intensity of nestin+ fluorescence staining on neural stem/progenitor cells during differentiation in vitro indicated no significant difference on any of the days tested (days 3, 10 and 20, P > 0.05; Figure 4).

Bottom Line: Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors.Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche.Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China ; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.

ABSTRACT
In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

No MeSH data available.


Related in: MedlinePlus