Limits...
Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro.

Lu J, Lu K, Li D - Neural Regen Res (2012)

Bottom Line: Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors.Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche.Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China ; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.

ABSTRACT
In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

No MeSH data available.


Related in: MedlinePlus

Dynamic expression of FGF8, FGFRs and Sonic Hedgehog signaling pathway molecule mRNA during neural stem/progenitor cells differentiation in vitro was measured on day, 10 and 20 by reverse transcription-PCR.aP < 0.05, vs. expression of the previous differentiation stage. The results were expressed as absorbance ratio of mRNA expression on day 10 or 20 to that of the neural stem/progenitor cell stage, which was assigned a value of 1 (mean ± SD, n = 4).FGF: Fibroblast growth factor; FGFR: fibroblast growth factor receptor; NSCs/NPCs: neural stem/progenitor cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4302448&req=5

Figure 1: Dynamic expression of FGF8, FGFRs and Sonic Hedgehog signaling pathway molecule mRNA during neural stem/progenitor cells differentiation in vitro was measured on day, 10 and 20 by reverse transcription-PCR.aP < 0.05, vs. expression of the previous differentiation stage. The results were expressed as absorbance ratio of mRNA expression on day 10 or 20 to that of the neural stem/progenitor cell stage, which was assigned a value of 1 (mean ± SD, n = 4).FGF: Fibroblast growth factor; FGFR: fibroblast growth factor receptor; NSCs/NPCs: neural stem/progenitor cells.

Mentions: Quantitative reverse transcription-PCR, western blot and enzyme-linked immunosorbent assay (ELISA) analyses demonstrated that the mean fibroblast growth factor 8 mRNA expression and protein levels from cultured neural stem/progenitor cells were significantly lower on day 10 of the differentiation culture compared with neural stem/progenitor cells or day 20 (P < 0.05; Figures 1, 2).


Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro.

Lu J, Lu K, Li D - Neural Regen Res (2012)

Dynamic expression of FGF8, FGFRs and Sonic Hedgehog signaling pathway molecule mRNA during neural stem/progenitor cells differentiation in vitro was measured on day, 10 and 20 by reverse transcription-PCR.aP < 0.05, vs. expression of the previous differentiation stage. The results were expressed as absorbance ratio of mRNA expression on day 10 or 20 to that of the neural stem/progenitor cell stage, which was assigned a value of 1 (mean ± SD, n = 4).FGF: Fibroblast growth factor; FGFR: fibroblast growth factor receptor; NSCs/NPCs: neural stem/progenitor cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302448&req=5

Figure 1: Dynamic expression of FGF8, FGFRs and Sonic Hedgehog signaling pathway molecule mRNA during neural stem/progenitor cells differentiation in vitro was measured on day, 10 and 20 by reverse transcription-PCR.aP < 0.05, vs. expression of the previous differentiation stage. The results were expressed as absorbance ratio of mRNA expression on day 10 or 20 to that of the neural stem/progenitor cell stage, which was assigned a value of 1 (mean ± SD, n = 4).FGF: Fibroblast growth factor; FGFR: fibroblast growth factor receptor; NSCs/NPCs: neural stem/progenitor cells.
Mentions: Quantitative reverse transcription-PCR, western blot and enzyme-linked immunosorbent assay (ELISA) analyses demonstrated that the mean fibroblast growth factor 8 mRNA expression and protein levels from cultured neural stem/progenitor cells were significantly lower on day 10 of the differentiation culture compared with neural stem/progenitor cells or day 20 (P < 0.05; Figures 1, 2).

Bottom Line: Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors.Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche.Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China ; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China.

ABSTRACT
In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fibroblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog expression may be related to the differentiation of neural stem/progenitor cells.

No MeSH data available.


Related in: MedlinePlus