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Effect of topical Nanoliposomes of Paromomycin on Rats Liver and Kidney.

Kalantari H, Hemmati AA, Bavarsad N, Rezaie A, Ahmadi S - Jundishapur J Nat Pharm Prod (2014)

Bottom Line: The results showed that no significant change was occurred in the amount of the above factors compared with the control group with the negative control group in 3 time periods (P > 0.05).The histopathological results of the liver and kidney showed that there was no difference in the between the negative control and positive control groups and the control group in the 10- and 20-day periods, and they had a normal structure.In general, it can be said that the application of nanoliposomal paromomycin sulfate formulations for topical treatment of the cutaneous leishmaniasis does not create serious side effects in the short term, but its long-term use leads to mild renal and liver side effects that requires more attention.

View Article: PubMed Central - PubMed

Affiliation: Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Center Medicinal Plants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Hepatotoxicity due to drugs is the most common cause of deaths. Nephrotoxicity of the drugs is usually associated with the drugs accumulation in renal tissue. Paromomycin sulfate (PMS) is an anti-leishmania drug. Although the topical approach for the treatment of leishmania is attractive, its use might cause nephrotoxicity and hepatotoxicity.

Objectives: This study aimed to evaluate probable nephrotoxicity and hepatotoxicity of topically administered PMS liposomes.

Materials and methods: Nine groups of male rats were used in this study; each group consisted of 6 rats that were evaluated in 3 time periods of 10, 20, and 30 days. Three groups were placed in each time period; a control group did not receive any medicine; a negative control group received liposome without paromomycin sulfate; and a positive control group received nanoliposomal formulations containing paromomycin sulfate. Pharmaceutical formulation (topical form) was used 2 times a day in a 12-hour interval. At the end of the period, hepatic enzymes (ALT, AST, and ALK), BUN levels, and serum creatinine were measured.

Results: The results showed that no significant change was occurred in the amount of the above factors compared with the control group with the negative control group in 3 time periods (P > 0.05). The histopathological results of the liver and kidney showed that there was no difference in the between the negative control and positive control groups and the control group in the 10- and 20-day periods, and they had a normal structure. However, after the 30-day time period a reversible cellular inflammation in the liver and mild kidney necrosis was seen in the positive control group versus the control and negative control groups.

Conclusions: In general, it can be said that the application of nanoliposomal paromomycin sulfate formulations for topical treatment of the cutaneous leishmaniasis does not create serious side effects in the short term, but its long-term use leads to mild renal and liver side effects that requires more attention.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of Liver, (H&E, Bar: 20 µm)A, control group; B, negative control (10 days); C, negative control (20 days); D, negative control (30 days); E, positive control (10 days); F, positive control (20 days). Note to the normal structure of liver (Figure 4 A-F) and hepatocytes which are located around the central vein (CV); G, positive control (30 days). Cell swelling in hepatocytes is obvious (arrows).
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fig14059: Photomicrographs of Liver, (H&E, Bar: 20 µm)A, control group; B, negative control (10 days); C, negative control (20 days); D, negative control (30 days); E, positive control (10 days); F, positive control (20 days). Note to the normal structure of liver (Figure 4 A-F) and hepatocytes which are located around the central vein (CV); G, positive control (30 days). Cell swelling in hepatocytes is obvious (arrows).

Mentions: Livers of the animals in control and negative control groups, which were sacrificed after 10, 20 and 30 days, showed a normal structure (Figures 1-4). Also, rats in the positive control group, which were euthanized in 10 and 20 days, had normal livers (Figures 5E and 5F). Histopathological inspection of the positive control group that was euthanized after 30 days, revealed severe cell swelling in hepatocytes (Figure 5G). Vacant spaces in hepatocytes were diffused, and enlarged.


Effect of topical Nanoliposomes of Paromomycin on Rats Liver and Kidney.

Kalantari H, Hemmati AA, Bavarsad N, Rezaie A, Ahmadi S - Jundishapur J Nat Pharm Prod (2014)

Photomicrographs of Liver, (H&E, Bar: 20 µm)A, control group; B, negative control (10 days); C, negative control (20 days); D, negative control (30 days); E, positive control (10 days); F, positive control (20 days). Note to the normal structure of liver (Figure 4 A-F) and hepatocytes which are located around the central vein (CV); G, positive control (30 days). Cell swelling in hepatocytes is obvious (arrows).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302405&req=5

fig14059: Photomicrographs of Liver, (H&E, Bar: 20 µm)A, control group; B, negative control (10 days); C, negative control (20 days); D, negative control (30 days); E, positive control (10 days); F, positive control (20 days). Note to the normal structure of liver (Figure 4 A-F) and hepatocytes which are located around the central vein (CV); G, positive control (30 days). Cell swelling in hepatocytes is obvious (arrows).
Mentions: Livers of the animals in control and negative control groups, which were sacrificed after 10, 20 and 30 days, showed a normal structure (Figures 1-4). Also, rats in the positive control group, which were euthanized in 10 and 20 days, had normal livers (Figures 5E and 5F). Histopathological inspection of the positive control group that was euthanized after 30 days, revealed severe cell swelling in hepatocytes (Figure 5G). Vacant spaces in hepatocytes were diffused, and enlarged.

Bottom Line: The results showed that no significant change was occurred in the amount of the above factors compared with the control group with the negative control group in 3 time periods (P > 0.05).The histopathological results of the liver and kidney showed that there was no difference in the between the negative control and positive control groups and the control group in the 10- and 20-day periods, and they had a normal structure.In general, it can be said that the application of nanoliposomal paromomycin sulfate formulations for topical treatment of the cutaneous leishmaniasis does not create serious side effects in the short term, but its long-term use leads to mild renal and liver side effects that requires more attention.

View Article: PubMed Central - PubMed

Affiliation: Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Center Medicinal Plants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

ABSTRACT

Background: Hepatotoxicity due to drugs is the most common cause of deaths. Nephrotoxicity of the drugs is usually associated with the drugs accumulation in renal tissue. Paromomycin sulfate (PMS) is an anti-leishmania drug. Although the topical approach for the treatment of leishmania is attractive, its use might cause nephrotoxicity and hepatotoxicity.

Objectives: This study aimed to evaluate probable nephrotoxicity and hepatotoxicity of topically administered PMS liposomes.

Materials and methods: Nine groups of male rats were used in this study; each group consisted of 6 rats that were evaluated in 3 time periods of 10, 20, and 30 days. Three groups were placed in each time period; a control group did not receive any medicine; a negative control group received liposome without paromomycin sulfate; and a positive control group received nanoliposomal formulations containing paromomycin sulfate. Pharmaceutical formulation (topical form) was used 2 times a day in a 12-hour interval. At the end of the period, hepatic enzymes (ALT, AST, and ALK), BUN levels, and serum creatinine were measured.

Results: The results showed that no significant change was occurred in the amount of the above factors compared with the control group with the negative control group in 3 time periods (P > 0.05). The histopathological results of the liver and kidney showed that there was no difference in the between the negative control and positive control groups and the control group in the 10- and 20-day periods, and they had a normal structure. However, after the 30-day time period a reversible cellular inflammation in the liver and mild kidney necrosis was seen in the positive control group versus the control and negative control groups.

Conclusions: In general, it can be said that the application of nanoliposomal paromomycin sulfate formulations for topical treatment of the cutaneous leishmaniasis does not create serious side effects in the short term, but its long-term use leads to mild renal and liver side effects that requires more attention.

No MeSH data available.


Related in: MedlinePlus