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Introducing Urtica dioica, A Native Plant of Khuzestan, As an Antibacterial Medicinal Plant.

Motamedi H, Seyyednejad SM, Bakhtiari A, Vafaei M - Jundishapur J Nat Pharm Prod (2014)

Bottom Line: The MBC was found only for S. epidermidis (20 mg/mL).Extracts of U. dioica showed significant antibacterial effect against some clinically important pathogenic bacteria.Based on the obtained results it can be concluded that U. dioica is useful as antibacterial and bactericidal agent in treating infectious diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran.

ABSTRACT

Background: Urtica dioica is a flowering plant with long history of use in folk medicine and as a food source.

Objectives: This study examined in vitro antibacterial potential of alcoholic extracts of U. dioica.

Materials and methods: Hydroalcoholic extracts from aerial parts were prepared using aqueous solution of ethanol and methanol and their inhibitory effects against clinical isolates was examined by disc diffusion method at different doses. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) indexes were also investigated. The scanning electron microscopy (SEM) analysis was also performed to find structural changes of affected bacteria consequent to exposing with extracts.

Results: Both extracts were active against Bacillus cereus, Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli with respectively 16, 10, 18, and 14 mm (methanolic) and 11, 9, 17, and 16 mm (ethanolic) inhibition zone. The MIC of ethanolic extract against S. epidermidis and E. coli was respectively 10 and 40 mg/mL. The MIC of methanolic extract against S. aureus and S. epidermidis was 40 and 10 mg/mL, respectively. The MBC was found only for S. epidermidis (20 mg/mL). In SEM analysis the round shape of S. epidermidis was changed and irregular shapes were appeared, which suggest that the main target of these extracts was cell wall.

Conclusions: Extracts of U. dioica showed significant antibacterial effect against some clinically important pathogenic bacteria. Based on the obtained results it can be concluded that U. dioica is useful as antibacterial and bactericidal agent in treating infectious diseases.

No MeSH data available.


Related in: MedlinePlus

Scanning Electron Microscopy Analysis of Structural changes of Staphylococcus epidermidis After Exposure with Methanolic ExtractThe arrows show deformed bacteria.
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fig13853: Scanning Electron Microscopy Analysis of Structural changes of Staphylococcus epidermidis After Exposure with Methanolic ExtractThe arrows show deformed bacteria.

Mentions: The antibacterial activity of ethanolic and methanolic extracts of U. dioica appeared as growth inhibition of tested bacteria. The results of the antimicrobial activity are given in the Tables 1 and 2. These results showed that these extracts were effective against four tested organisms including E. coli, S. epidermidis, B. cereus, and S. aureus. The highest activity (inhibition zone diameter about 18 mm) was demonstrated by the methanolic extract of U. dioica leaves against S. epidermidis while the lowest activity (inhibition zone diameter about 7 mm) was demonstrated by the ethanolic extract against B. cereus and S. aureus and by methanolic extract against B. cereus. On the other hand, the ethanolic and methanolic extracts were not active against S. Typhi, P. aeruginosa, K. pneumoniae, and P. mirabilis. The results of MIC and MBC of ethanolic and methanolic extracts for three bacterial species are shown in Table 3. Time-kill kinetic for methanolic extract of U. dioica was nine hours (Table 4). Figure 1 shows the structural changes of S. epidermidis following exposure to alcoholic extract. As we can found the round shape of this bacterium was changed to ovoid or irregular forms. Some of them were collapsed and appear to be somewhat indented. The size of the affected bacteria was also decreased and varied.


Introducing Urtica dioica, A Native Plant of Khuzestan, As an Antibacterial Medicinal Plant.

Motamedi H, Seyyednejad SM, Bakhtiari A, Vafaei M - Jundishapur J Nat Pharm Prod (2014)

Scanning Electron Microscopy Analysis of Structural changes of Staphylococcus epidermidis After Exposure with Methanolic ExtractThe arrows show deformed bacteria.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4302403&req=5

fig13853: Scanning Electron Microscopy Analysis of Structural changes of Staphylococcus epidermidis After Exposure with Methanolic ExtractThe arrows show deformed bacteria.
Mentions: The antibacterial activity of ethanolic and methanolic extracts of U. dioica appeared as growth inhibition of tested bacteria. The results of the antimicrobial activity are given in the Tables 1 and 2. These results showed that these extracts were effective against four tested organisms including E. coli, S. epidermidis, B. cereus, and S. aureus. The highest activity (inhibition zone diameter about 18 mm) was demonstrated by the methanolic extract of U. dioica leaves against S. epidermidis while the lowest activity (inhibition zone diameter about 7 mm) was demonstrated by the ethanolic extract against B. cereus and S. aureus and by methanolic extract against B. cereus. On the other hand, the ethanolic and methanolic extracts were not active against S. Typhi, P. aeruginosa, K. pneumoniae, and P. mirabilis. The results of MIC and MBC of ethanolic and methanolic extracts for three bacterial species are shown in Table 3. Time-kill kinetic for methanolic extract of U. dioica was nine hours (Table 4). Figure 1 shows the structural changes of S. epidermidis following exposure to alcoholic extract. As we can found the round shape of this bacterium was changed to ovoid or irregular forms. Some of them were collapsed and appear to be somewhat indented. The size of the affected bacteria was also decreased and varied.

Bottom Line: The MBC was found only for S. epidermidis (20 mg/mL).Extracts of U. dioica showed significant antibacterial effect against some clinically important pathogenic bacteria.Based on the obtained results it can be concluded that U. dioica is useful as antibacterial and bactericidal agent in treating infectious diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran.

ABSTRACT

Background: Urtica dioica is a flowering plant with long history of use in folk medicine and as a food source.

Objectives: This study examined in vitro antibacterial potential of alcoholic extracts of U. dioica.

Materials and methods: Hydroalcoholic extracts from aerial parts were prepared using aqueous solution of ethanol and methanol and their inhibitory effects against clinical isolates was examined by disc diffusion method at different doses. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) indexes were also investigated. The scanning electron microscopy (SEM) analysis was also performed to find structural changes of affected bacteria consequent to exposing with extracts.

Results: Both extracts were active against Bacillus cereus, Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli with respectively 16, 10, 18, and 14 mm (methanolic) and 11, 9, 17, and 16 mm (ethanolic) inhibition zone. The MIC of ethanolic extract against S. epidermidis and E. coli was respectively 10 and 40 mg/mL. The MIC of methanolic extract against S. aureus and S. epidermidis was 40 and 10 mg/mL, respectively. The MBC was found only for S. epidermidis (20 mg/mL). In SEM analysis the round shape of S. epidermidis was changed and irregular shapes were appeared, which suggest that the main target of these extracts was cell wall.

Conclusions: Extracts of U. dioica showed significant antibacterial effect against some clinically important pathogenic bacteria. Based on the obtained results it can be concluded that U. dioica is useful as antibacterial and bactericidal agent in treating infectious diseases.

No MeSH data available.


Related in: MedlinePlus