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Long non-coding RNA PVT1 is associated with tumor progression and predicts recurrence in hepatocellular carcinoma patients.

Ding C, Yang Z, Lv Z, DU C, Xiao H, Peng C, Cheng S, Xie H, Zhou L, Wu J, Zheng S - Oncol Lett (2014)

Bottom Line: PVT1, which maps to chromosome 8q24, is a copy number amplification-associated long non-coding RNA.The relative expression levels of PVT1 were significantly higher in cancerous tissues compared with the corresponding non-cancerous tissues (cohort one, P=0.0016; cohort two, P=0.0274).Furthermore, overexpression of PVT1 was associated with a higher serum α-fetoprotein expression level (P=0.011) and a higher recurrence rate (P=0.004).

View Article: PubMed Central - PubMed

Affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310003, P.R. China ; Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou, Zhejiang 310003, P.R. China.

ABSTRACT

PVT1, which maps to chromosome 8q24, is a copy number amplification-associated long non-coding RNA. Overexpression of PVT1 is a powerful predictor of tumor progression and patient survival in a diverse range of cancer types. However, the association between PVT1 and hepatocellular carcinoma (HCC) remains unclear. The aim of the present study was to examine the expression pattern of PVT1, and its clinical significance in HCC. Between 2003 and 2012, reverse transcription-quantitative polymerase chain reaction was used to determine the expression levels of PVT1 in two independent cohorts: Cohort one, 58 HCC resection samples; and cohort 2, 214 HCC transplant samples. Additionally, the correlation between PVT1 expression levels and clinical parameters and outcomes was analyzed. The relative expression levels of PVT1 were significantly higher in cancerous tissues compared with the corresponding non-cancerous tissues (cohort one, P=0.0016; cohort two, P=0.0274). Furthermore, overexpression of PVT1 was associated with a higher serum α-fetoprotein expression level (P=0.011) and a higher recurrence rate (P=0.004). Kaplan-Meier analysis indicated that the patients with high PVT1 expression exhibited poor recurrence-free survival (P=0.021), and multivariate analysis demonstrated that high levels of PVT1 expression are an independent predictor for HCC recurrence (P=0.042; hazard ratio, 1.653). Thus, the high expression levels of PVT1 in HCC may serve as a novel biomarker for predicting tumor recurrence in HCC patients, and as a potential therapeutic target.

No MeSH data available.


Related in: MedlinePlus

Relative PVT1 expression levels in 10 liver cancer cell lines. HL-7702 is a healthy liver cell line. Data are the mean ± standard error of the mean (n=3). *P<0.05, vs. the control (HL-7702 cells), according to the paired t-test.
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f2-ol-09-02-0955: Relative PVT1 expression levels in 10 liver cancer cell lines. HL-7702 is a healthy liver cell line. Data are the mean ± standard error of the mean (n=3). *P<0.05, vs. the control (HL-7702 cells), according to the paired t-test.

Mentions: In the present study, the expression levels of PVT1 in the 272 HCC patients from the two independent cohorts were determined using RT-qPCR. Compared with the corresponding non-tumor liver tissues of the HCC cohorts, PVT1 expression was significantly increased in the cancerous tissues of the patients in cohort one (P=0.0016; Fig. 1A) and cohort two (P=0.0274; Fig. 1B). Furthermore, the expression of PVT1 was defined in 10 liver cancer cell lines and one healthy liver cell line. Of the 10 liver cancer cell lines, six (HepG2, SK-Hep1, MHCC-97L, SMMC-7721, MHCC-97H and Bel-7402) expressed a higher level of PVT1 than the healthy liver cell line (Fig. 2). Thus, the present study determined that PVT1 expression was significantly increased in HCC.


Long non-coding RNA PVT1 is associated with tumor progression and predicts recurrence in hepatocellular carcinoma patients.

Ding C, Yang Z, Lv Z, DU C, Xiao H, Peng C, Cheng S, Xie H, Zhou L, Wu J, Zheng S - Oncol Lett (2014)

Relative PVT1 expression levels in 10 liver cancer cell lines. HL-7702 is a healthy liver cell line. Data are the mean ± standard error of the mean (n=3). *P<0.05, vs. the control (HL-7702 cells), according to the paired t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4301564&req=5

f2-ol-09-02-0955: Relative PVT1 expression levels in 10 liver cancer cell lines. HL-7702 is a healthy liver cell line. Data are the mean ± standard error of the mean (n=3). *P<0.05, vs. the control (HL-7702 cells), according to the paired t-test.
Mentions: In the present study, the expression levels of PVT1 in the 272 HCC patients from the two independent cohorts were determined using RT-qPCR. Compared with the corresponding non-tumor liver tissues of the HCC cohorts, PVT1 expression was significantly increased in the cancerous tissues of the patients in cohort one (P=0.0016; Fig. 1A) and cohort two (P=0.0274; Fig. 1B). Furthermore, the expression of PVT1 was defined in 10 liver cancer cell lines and one healthy liver cell line. Of the 10 liver cancer cell lines, six (HepG2, SK-Hep1, MHCC-97L, SMMC-7721, MHCC-97H and Bel-7402) expressed a higher level of PVT1 than the healthy liver cell line (Fig. 2). Thus, the present study determined that PVT1 expression was significantly increased in HCC.

Bottom Line: PVT1, which maps to chromosome 8q24, is a copy number amplification-associated long non-coding RNA.The relative expression levels of PVT1 were significantly higher in cancerous tissues compared with the corresponding non-cancerous tissues (cohort one, P=0.0016; cohort two, P=0.0274).Furthermore, overexpression of PVT1 was associated with a higher serum α-fetoprotein expression level (P=0.011) and a higher recurrence rate (P=0.004).

View Article: PubMed Central - PubMed

Affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang 310003, P.R. China ; Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou, Zhejiang 310003, P.R. China.

ABSTRACT

PVT1, which maps to chromosome 8q24, is a copy number amplification-associated long non-coding RNA. Overexpression of PVT1 is a powerful predictor of tumor progression and patient survival in a diverse range of cancer types. However, the association between PVT1 and hepatocellular carcinoma (HCC) remains unclear. The aim of the present study was to examine the expression pattern of PVT1, and its clinical significance in HCC. Between 2003 and 2012, reverse transcription-quantitative polymerase chain reaction was used to determine the expression levels of PVT1 in two independent cohorts: Cohort one, 58 HCC resection samples; and cohort 2, 214 HCC transplant samples. Additionally, the correlation between PVT1 expression levels and clinical parameters and outcomes was analyzed. The relative expression levels of PVT1 were significantly higher in cancerous tissues compared with the corresponding non-cancerous tissues (cohort one, P=0.0016; cohort two, P=0.0274). Furthermore, overexpression of PVT1 was associated with a higher serum α-fetoprotein expression level (P=0.011) and a higher recurrence rate (P=0.004). Kaplan-Meier analysis indicated that the patients with high PVT1 expression exhibited poor recurrence-free survival (P=0.021), and multivariate analysis demonstrated that high levels of PVT1 expression are an independent predictor for HCC recurrence (P=0.042; hazard ratio, 1.653). Thus, the high expression levels of PVT1 in HCC may serve as a novel biomarker for predicting tumor recurrence in HCC patients, and as a potential therapeutic target.

No MeSH data available.


Related in: MedlinePlus