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Antitumor effect of D-erythrose in an abdominal metastatic model of colon carcinoma.

Liu LL, Yi T, Zhao X - Oncol Lett (2014)

Bottom Line: The results revealed that D-erythrose significantly reduced the weight of the intraperitoneal tumor by 69.1%, markedly inhibited the development of ascites and increased tumor cell apoptosis, without any observed toxic effects.These observations suggest that D-erythrose possesses antitumor activity against colon cancer.The present study may provide a potentially effective and specific approach for colon cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetric, Gynecological and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

ABSTRACT

Traditional chemotherapy drugs against colorectal cancer possess little or no specificity, leading to severe intolerable side-effects. Therefore, it is necessary to develop additional specific therapeutic strategies. It has been suggested that D-erythrose may specifically inhibit the growth of tumor cells. However, the in vivo antitumor effect of D-erythrose against colorectal cancer remains unknown. Thus, the present study investigated the antitumor effect of D-erythrose in an abdominal metastatic model of colon carcinoma. Intraperitoneal (IP) colon carcinoma-bearing BALB/c mice received an IP injection of D-erythrose or normal saline (NS) daily for 15 days. The mice were weighed every three days. The tumor weights and the volume of ascites were evaluated following the treatment. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay was used to assess apoptosis in tumor tissues. The results revealed that D-erythrose significantly reduced the weight of the intraperitoneal tumor by 69.1%, markedly inhibited the development of ascites and increased tumor cell apoptosis, without any observed toxic effects. These observations suggest that D-erythrose possesses antitumor activity against colon cancer. The present study may provide a potentially effective and specific approach for colon cancer treatment.

No MeSH data available.


Related in: MedlinePlus

TUNEL staining of tumor sections in each group. (A) Tumor sections stained for TUNEL (magnification, ×200). Numerous TUNEL-positive cells were observed in the D-erythrose treated group, whereas such cells were rare in the NS group. (B) Quantification of TUNEL staining (apoptotic index). The data are expressed as the mean ± standard error. **P<0.01, vs. the NS group. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; NS, normal saline.
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f3-ol-09-02-0769: TUNEL staining of tumor sections in each group. (A) Tumor sections stained for TUNEL (magnification, ×200). Numerous TUNEL-positive cells were observed in the D-erythrose treated group, whereas such cells were rare in the NS group. (B) Quantification of TUNEL staining (apoptotic index). The data are expressed as the mean ± standard error. **P<0.01, vs. the NS group. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; NS, normal saline.

Mentions: A TUNEL assay was used to detect apoptotic tumor cells in each group. The cell nuclei stained with green fluorescence were considered to be TUNEL-positive nuclei. As shown in Fig. 3, the percentage of TUNEL-positive cells (apoptotic cells) was significantly increased in the D-erythrose treated group compared with that in the NS group (P<0.01). Apoptotic cells were rare in the NS group.


Antitumor effect of D-erythrose in an abdominal metastatic model of colon carcinoma.

Liu LL, Yi T, Zhao X - Oncol Lett (2014)

TUNEL staining of tumor sections in each group. (A) Tumor sections stained for TUNEL (magnification, ×200). Numerous TUNEL-positive cells were observed in the D-erythrose treated group, whereas such cells were rare in the NS group. (B) Quantification of TUNEL staining (apoptotic index). The data are expressed as the mean ± standard error. **P<0.01, vs. the NS group. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; NS, normal saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4301512&req=5

f3-ol-09-02-0769: TUNEL staining of tumor sections in each group. (A) Tumor sections stained for TUNEL (magnification, ×200). Numerous TUNEL-positive cells were observed in the D-erythrose treated group, whereas such cells were rare in the NS group. (B) Quantification of TUNEL staining (apoptotic index). The data are expressed as the mean ± standard error. **P<0.01, vs. the NS group. TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; NS, normal saline.
Mentions: A TUNEL assay was used to detect apoptotic tumor cells in each group. The cell nuclei stained with green fluorescence were considered to be TUNEL-positive nuclei. As shown in Fig. 3, the percentage of TUNEL-positive cells (apoptotic cells) was significantly increased in the D-erythrose treated group compared with that in the NS group (P<0.01). Apoptotic cells were rare in the NS group.

Bottom Line: The results revealed that D-erythrose significantly reduced the weight of the intraperitoneal tumor by 69.1%, markedly inhibited the development of ascites and increased tumor cell apoptosis, without any observed toxic effects.These observations suggest that D-erythrose possesses antitumor activity against colon cancer.The present study may provide a potentially effective and specific approach for colon cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetric, Gynecological and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

ABSTRACT

Traditional chemotherapy drugs against colorectal cancer possess little or no specificity, leading to severe intolerable side-effects. Therefore, it is necessary to develop additional specific therapeutic strategies. It has been suggested that D-erythrose may specifically inhibit the growth of tumor cells. However, the in vivo antitumor effect of D-erythrose against colorectal cancer remains unknown. Thus, the present study investigated the antitumor effect of D-erythrose in an abdominal metastatic model of colon carcinoma. Intraperitoneal (IP) colon carcinoma-bearing BALB/c mice received an IP injection of D-erythrose or normal saline (NS) daily for 15 days. The mice were weighed every three days. The tumor weights and the volume of ascites were evaluated following the treatment. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay was used to assess apoptosis in tumor tissues. The results revealed that D-erythrose significantly reduced the weight of the intraperitoneal tumor by 69.1%, markedly inhibited the development of ascites and increased tumor cell apoptosis, without any observed toxic effects. These observations suggest that D-erythrose possesses antitumor activity against colon cancer. The present study may provide a potentially effective and specific approach for colon cancer treatment.

No MeSH data available.


Related in: MedlinePlus