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microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2.

Long LM, He BF, Huang GQ, Guo YH, Liu YS, Huo JR - Oncol Lett (2014)

Bottom Line: Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis.A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly.In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

ABSTRACT

microRNAs (miRNAs/miRs) are a conserved class of endogenous, short non-coding RNAs that post-transcriptionally regulate the expression of genes involved in diverse cellular processes. miR-214 has been reported to be associated with several cancers, including human colon cancer. However, the function of miR-214 in colon cancer development is poorly understood. In the current study, miR-214 was demonstrated to be downregulated in colon cancer tissues compared with healthy colon tissues. Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis. ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214. A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly. In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

No MeSH data available.


Related in: MedlinePlus

miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.
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f1-ol-09-02-0645: miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.

Mentions: To investigate the function of miR-214 in human colon cancer development, miRNAMap2.0 (12) was used for the analysis of miR-214 in diverse normal tissues and tumor tissues, including colon cancer tissues. As shown in Fig. 1A, miR-214 was found to be downregulated in colon cancer. Based on the analysis of miRNAmap2.0, qPCR was performed to detect miR-214 expression in 24 paired normal and colon cancer tissues (Fig. 1B). miR-214 expression was found to be downregulated in colon cancer. These results indicate that the abnormal expression of miR-214 may be significant in colon cancer.


microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2.

Long LM, He BF, Huang GQ, Guo YH, Liu YS, Huo JR - Oncol Lett (2014)

miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4301474&req=5

f1-ol-09-02-0645: miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.
Mentions: To investigate the function of miR-214 in human colon cancer development, miRNAMap2.0 (12) was used for the analysis of miR-214 in diverse normal tissues and tumor tissues, including colon cancer tissues. As shown in Fig. 1A, miR-214 was found to be downregulated in colon cancer. Based on the analysis of miRNAmap2.0, qPCR was performed to detect miR-214 expression in 24 paired normal and colon cancer tissues (Fig. 1B). miR-214 expression was found to be downregulated in colon cancer. These results indicate that the abnormal expression of miR-214 may be significant in colon cancer.

Bottom Line: Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis.A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly.In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

ABSTRACT

microRNAs (miRNAs/miRs) are a conserved class of endogenous, short non-coding RNAs that post-transcriptionally regulate the expression of genes involved in diverse cellular processes. miR-214 has been reported to be associated with several cancers, including human colon cancer. However, the function of miR-214 in colon cancer development is poorly understood. In the current study, miR-214 was demonstrated to be downregulated in colon cancer tissues compared with healthy colon tissues. Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis. ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214. A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly. In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

No MeSH data available.


Related in: MedlinePlus