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microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2.

Long LM, He BF, Huang GQ, Guo YH, Liu YS, Huo JR - Oncol Lett (2014)

Bottom Line: Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis.ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214.In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

ABSTRACT

microRNAs (miRNAs/miRs) are a conserved class of endogenous, short non-coding RNAs that post-transcriptionally regulate the expression of genes involved in diverse cellular processes. miR-214 has been reported to be associated with several cancers, including human colon cancer. However, the function of miR-214 in colon cancer development is poorly understood. In the current study, miR-214 was demonstrated to be downregulated in colon cancer tissues compared with healthy colon tissues. Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis. ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214. A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly. In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

No MeSH data available.


Related in: MedlinePlus

miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.
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f1-ol-09-02-0645: miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.

Mentions: To investigate the function of miR-214 in human colon cancer development, miRNAMap2.0 (12) was used for the analysis of miR-214 in diverse normal tissues and tumor tissues, including colon cancer tissues. As shown in Fig. 1A, miR-214 was found to be downregulated in colon cancer. Based on the analysis of miRNAmap2.0, qPCR was performed to detect miR-214 expression in 24 paired normal and colon cancer tissues (Fig. 1B). miR-214 expression was found to be downregulated in colon cancer. These results indicate that the abnormal expression of miR-214 may be significant in colon cancer.


microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2.

Long LM, He BF, Huang GQ, Guo YH, Liu YS, Huo JR - Oncol Lett (2014)

miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4301474&req=5

f1-ol-09-02-0645: miR-214 downregulation in human colon cancer tissues. (A) The expression of miR-214 was analyzed using miRNAMap-2.0 among diverse normal tissues and cancer tissues, including colon cancer tissues. The color shades represent the relative expression levels of miR-214. (B) miR-214 expression was quantified by qPCR in 24 paired colon cancer tissues and normal tissues. U6 was used as an internal control. *P<0.05. snRNA, small nuclear RNA; miRNA/miR, microRNA; qPCR, quantitative polymerase chain reaction.
Mentions: To investigate the function of miR-214 in human colon cancer development, miRNAMap2.0 (12) was used for the analysis of miR-214 in diverse normal tissues and tumor tissues, including colon cancer tissues. As shown in Fig. 1A, miR-214 was found to be downregulated in colon cancer. Based on the analysis of miRNAmap2.0, qPCR was performed to detect miR-214 expression in 24 paired normal and colon cancer tissues (Fig. 1B). miR-214 expression was found to be downregulated in colon cancer. These results indicate that the abnormal expression of miR-214 may be significant in colon cancer.

Bottom Line: Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis.ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214.In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

ABSTRACT

microRNAs (miRNAs/miRs) are a conserved class of endogenous, short non-coding RNAs that post-transcriptionally regulate the expression of genes involved in diverse cellular processes. miR-214 has been reported to be associated with several cancers, including human colon cancer. However, the function of miR-214 in colon cancer development is poorly understood. In the current study, miR-214 was demonstrated to be downregulated in colon cancer tissues compared with healthy colon tissues. Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis. ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214. A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly. In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.

No MeSH data available.


Related in: MedlinePlus