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On the Sulfation Pattern of Polysaccharides in the Extracellular Matrix of Sheep with Chondrodysplasia.

Piripi SA, Williams MA, Thompson KG - Cartilage (2011)

Bottom Line: Several forms of chondrodysplasia, a condition resulting in clear macroscopic deficiencies in the mechanical properties of the cartilage and characterized by reduced levels of sulfate, have been identified in both human beings and animals.Significantly, cartilage from affected sheep had a lower ratio of the chondroitin-derived enzymatically liberated disaccharides Δdi-mono4S to Δdi-mono6S, demonstrating reduced levels of chondroitin 4-sulfate, but not chondroitin 6-sulfate, in chondrodysplastic sheep compared to age-matched controls at all ages measured.This supports the hypothesis that a difference in chondroitin sulfate disaccharides is detectable in affected newborn lambs prior to the development of lesions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Veterinary, Animal & Biomedical Sciences, Massey University, Palmerston North, New Zealand.

ABSTRACT

Objective: Chondroitin sulfate is the major sulfated polysaccharide attached to the core protein, aggrecan, in the hyaline cartilage matrix. Sulfation of the cartilage matrix polysaccharide is vital for normal matrix integrity and compressive stiffness of the tissue and is therefore crucial to normal cartilage formation and consequently to endochondral ossification. Several forms of chondrodysplasia, a condition resulting in clear macroscopic deficiencies in the mechanical properties of the cartilage and characterized by reduced levels of sulfate, have been identified in both human beings and animals.

Design: In this study, the authors used capillary electrophoresis to investigate the sulfation state of extracted chondroitin sulfate polymers.

Results: Significantly, cartilage from affected sheep had a lower ratio of the chondroitin-derived enzymatically liberated disaccharides Δdi-mono4S to Δdi-mono6S, demonstrating reduced levels of chondroitin 4-sulfate, but not chondroitin 6-sulfate, in chondrodysplastic sheep compared to age-matched controls at all ages measured.

Conclusion: This supports the hypothesis that a difference in chondroitin sulfate disaccharides is detectable in affected newborn lambs prior to the development of lesions.

No MeSH data available.


Related in: MedlinePlus

Typical electropherogram of chondroitin disaccharides from ovine articular cartilage. (A) Double peaks result from anomeric forms of hexosamine. (B) Different ages of background buffer alter the resolution of these anomeric forms. 6S indicates Δdi-mono6S, 4S indicates Δdi-mono4S, and 0S represents unsulfated chondroitin disaccharide.
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fig1-1947603510377465: Typical electropherogram of chondroitin disaccharides from ovine articular cartilage. (A) Double peaks result from anomeric forms of hexosamine. (B) Different ages of background buffer alter the resolution of these anomeric forms. 6S indicates Δdi-mono6S, 4S indicates Δdi-mono4S, and 0S represents unsulfated chondroitin disaccharide.

Mentions: A typical electropherogram is shown in Figure 1, demonstrating peaks caused by Δdi-mono4S, Δdi-mono6S, and a peak at the expected location for unsulfated chondroitin disaccharide, as demonstrated by Karamanos et al.11 Double peaks were routinely observed, owing to the different anomeric forms of the hexosamine present at the reducing terminal of the disaccharide. Ten milligrams of each sample was run and mean concentrations of disaccharide for each of the tested animals are listed in Table 1, expressed as ng/10-mg sample. Concentrations of Δdi-mono4S were markedly lower in cartilage from chondrodysplastic sheep of all ages compared with controls, but there was no such difference in Δdi-mono6S concentrations. The ratios of Δdi-mono4S to Δdi-mono6S were also lower in chondrodysplastic sheep than in unrelated control animals in all age groups (P ≤ 0.0001; Fig. 2). In addition, the ratio of Δdi-mono4S to Δdi-mono6S was markedly lower in the mature control than in the younger controls and in the mature affected ram than in the younger affected lambs (P ≤ 0.0001). The concentration of unsulfated chondroitin disaccharide did not vary significantly with disease status.


On the Sulfation Pattern of Polysaccharides in the Extracellular Matrix of Sheep with Chondrodysplasia.

Piripi SA, Williams MA, Thompson KG - Cartilage (2011)

Typical electropherogram of chondroitin disaccharides from ovine articular cartilage. (A) Double peaks result from anomeric forms of hexosamine. (B) Different ages of background buffer alter the resolution of these anomeric forms. 6S indicates Δdi-mono6S, 4S indicates Δdi-mono4S, and 0S represents unsulfated chondroitin disaccharide.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4300791&req=5

fig1-1947603510377465: Typical electropherogram of chondroitin disaccharides from ovine articular cartilage. (A) Double peaks result from anomeric forms of hexosamine. (B) Different ages of background buffer alter the resolution of these anomeric forms. 6S indicates Δdi-mono6S, 4S indicates Δdi-mono4S, and 0S represents unsulfated chondroitin disaccharide.
Mentions: A typical electropherogram is shown in Figure 1, demonstrating peaks caused by Δdi-mono4S, Δdi-mono6S, and a peak at the expected location for unsulfated chondroitin disaccharide, as demonstrated by Karamanos et al.11 Double peaks were routinely observed, owing to the different anomeric forms of the hexosamine present at the reducing terminal of the disaccharide. Ten milligrams of each sample was run and mean concentrations of disaccharide for each of the tested animals are listed in Table 1, expressed as ng/10-mg sample. Concentrations of Δdi-mono4S were markedly lower in cartilage from chondrodysplastic sheep of all ages compared with controls, but there was no such difference in Δdi-mono6S concentrations. The ratios of Δdi-mono4S to Δdi-mono6S were also lower in chondrodysplastic sheep than in unrelated control animals in all age groups (P ≤ 0.0001; Fig. 2). In addition, the ratio of Δdi-mono4S to Δdi-mono6S was markedly lower in the mature control than in the younger controls and in the mature affected ram than in the younger affected lambs (P ≤ 0.0001). The concentration of unsulfated chondroitin disaccharide did not vary significantly with disease status.

Bottom Line: Several forms of chondrodysplasia, a condition resulting in clear macroscopic deficiencies in the mechanical properties of the cartilage and characterized by reduced levels of sulfate, have been identified in both human beings and animals.Significantly, cartilage from affected sheep had a lower ratio of the chondroitin-derived enzymatically liberated disaccharides Δdi-mono4S to Δdi-mono6S, demonstrating reduced levels of chondroitin 4-sulfate, but not chondroitin 6-sulfate, in chondrodysplastic sheep compared to age-matched controls at all ages measured.This supports the hypothesis that a difference in chondroitin sulfate disaccharides is detectable in affected newborn lambs prior to the development of lesions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Veterinary, Animal & Biomedical Sciences, Massey University, Palmerston North, New Zealand.

ABSTRACT

Objective: Chondroitin sulfate is the major sulfated polysaccharide attached to the core protein, aggrecan, in the hyaline cartilage matrix. Sulfation of the cartilage matrix polysaccharide is vital for normal matrix integrity and compressive stiffness of the tissue and is therefore crucial to normal cartilage formation and consequently to endochondral ossification. Several forms of chondrodysplasia, a condition resulting in clear macroscopic deficiencies in the mechanical properties of the cartilage and characterized by reduced levels of sulfate, have been identified in both human beings and animals.

Design: In this study, the authors used capillary electrophoresis to investigate the sulfation state of extracted chondroitin sulfate polymers.

Results: Significantly, cartilage from affected sheep had a lower ratio of the chondroitin-derived enzymatically liberated disaccharides Δdi-mono4S to Δdi-mono6S, demonstrating reduced levels of chondroitin 4-sulfate, but not chondroitin 6-sulfate, in chondrodysplastic sheep compared to age-matched controls at all ages measured.

Conclusion: This supports the hypothesis that a difference in chondroitin sulfate disaccharides is detectable in affected newborn lambs prior to the development of lesions.

No MeSH data available.


Related in: MedlinePlus