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The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus

Collagen I, II, and X expression of micro mass cultures of mesenchymal stem cells (MSCs) and moderate and severely affected osteoarthritis (OA) chondrocytes. (a) Expression of Col I, Col II, and Col X of micro mass cultures of MSCs and chondrocytes from moderately affected OA tissue, grown with FGF2v1. (b) Ratio of Col II/Col I in micro mass cultures of MSCs or chondrocytes derived from moderate or severely OA tissue. Mean and standard deviation values are presented in each graph. Changes in relative expression were analyzed with a 2-tailed paired t test (21-24 patients) except when the pairing was not significantly effective, and then the 2-tailed Mann Whitney test was performed (marked with *). The statistical tests were performed as described in Materials and Methods, and significant P values are shown.
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fig6-1947603510380899: Collagen I, II, and X expression of micro mass cultures of mesenchymal stem cells (MSCs) and moderate and severely affected osteoarthritis (OA) chondrocytes. (a) Expression of Col I, Col II, and Col X of micro mass cultures of MSCs and chondrocytes from moderately affected OA tissue, grown with FGF2v1. (b) Ratio of Col II/Col I in micro mass cultures of MSCs or chondrocytes derived from moderate or severely OA tissue. Mean and standard deviation values are presented in each graph. Changes in relative expression were analyzed with a 2-tailed paired t test (21-24 patients) except when the pairing was not significantly effective, and then the 2-tailed Mann Whitney test was performed (marked with *). The statistical tests were performed as described in Materials and Methods, and significant P values are shown.

Mentions: The expression of Col I and Col II was systematically analyzed by qPCR on RNA purified from micro mass cultures of both chondrocytes and MSCs derived from each subject (Figs. 5, 6). Addition of FGF2v1 to the chondrocyte cultures dramatically lowered the expression levels of Col I (Fig. 5a): 3.6-fold for the moderate OA-derived cells (P = 0.033) and 3-fold for the severe OA-derived cells (P = 0.036). Col II, the hallmark of hyaline cartilage (Fig. 5b), was also significantly increased in micro mass cultured chondrocytes expanded in the presence of FGF2v1 compared with those expanded without the growth factor (P = 0.0021 and P = 0.013 for moderate and severely OA affected tissue, respectively). Interestingly, there was no significant difference in Col II expression in FGF2v1-treated chondrocytes between those derived from moderately or severely affected OA tissue (Fig. 5b).


The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Collagen I, II, and X expression of micro mass cultures of mesenchymal stem cells (MSCs) and moderate and severely affected osteoarthritis (OA) chondrocytes. (a) Expression of Col I, Col II, and Col X of micro mass cultures of MSCs and chondrocytes from moderately affected OA tissue, grown with FGF2v1. (b) Ratio of Col II/Col I in micro mass cultures of MSCs or chondrocytes derived from moderate or severely OA tissue. Mean and standard deviation values are presented in each graph. Changes in relative expression were analyzed with a 2-tailed paired t test (21-24 patients) except when the pairing was not significantly effective, and then the 2-tailed Mann Whitney test was performed (marked with *). The statistical tests were performed as described in Materials and Methods, and significant P values are shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4300788&req=5

fig6-1947603510380899: Collagen I, II, and X expression of micro mass cultures of mesenchymal stem cells (MSCs) and moderate and severely affected osteoarthritis (OA) chondrocytes. (a) Expression of Col I, Col II, and Col X of micro mass cultures of MSCs and chondrocytes from moderately affected OA tissue, grown with FGF2v1. (b) Ratio of Col II/Col I in micro mass cultures of MSCs or chondrocytes derived from moderate or severely OA tissue. Mean and standard deviation values are presented in each graph. Changes in relative expression were analyzed with a 2-tailed paired t test (21-24 patients) except when the pairing was not significantly effective, and then the 2-tailed Mann Whitney test was performed (marked with *). The statistical tests were performed as described in Materials and Methods, and significant P values are shown.
Mentions: The expression of Col I and Col II was systematically analyzed by qPCR on RNA purified from micro mass cultures of both chondrocytes and MSCs derived from each subject (Figs. 5, 6). Addition of FGF2v1 to the chondrocyte cultures dramatically lowered the expression levels of Col I (Fig. 5a): 3.6-fold for the moderate OA-derived cells (P = 0.033) and 3-fold for the severe OA-derived cells (P = 0.036). Col II, the hallmark of hyaline cartilage (Fig. 5b), was also significantly increased in micro mass cultured chondrocytes expanded in the presence of FGF2v1 compared with those expanded without the growth factor (P = 0.0021 and P = 0.013 for moderate and severely OA affected tissue, respectively). Interestingly, there was no significant difference in Col II expression in FGF2v1-treated chondrocytes between those derived from moderately or severely affected OA tissue (Fig. 5b).

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus