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The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus

Histology sections of micro mass cultures of mesenchymal stem cells (MSCs) and moderate or severely affected chondrocytes. Micro mass cultures of MSCs (hBM-MSC) or chondrocytes (hAC) derived from moderate or severely affected osteoarthritis tissue from a representative subject were analyzed by histology (hematoxylin and eosin [H&E] stain; bars = 100 µm) with (F2v1) or without (no ligand) the presence of FGF2v1 in the culture medium used to expand the cells. The presence of proteoglycans and sulfated glycosaminoglycans was examined by Alcian blue (AB) and safranin O (SO) stains (bars = 100 µm).
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fig4-1947603510380899: Histology sections of micro mass cultures of mesenchymal stem cells (MSCs) and moderate or severely affected chondrocytes. Micro mass cultures of MSCs (hBM-MSC) or chondrocytes (hAC) derived from moderate or severely affected osteoarthritis tissue from a representative subject were analyzed by histology (hematoxylin and eosin [H&E] stain; bars = 100 µm) with (F2v1) or without (no ligand) the presence of FGF2v1 in the culture medium used to expand the cells. The presence of proteoglycans and sulfated glycosaminoglycans was examined by Alcian blue (AB) and safranin O (SO) stains (bars = 100 µm).

Mentions: A representative histological analysis of the micro mass cultures is shown in Figure 4. Larger pellets were obtained for chondrocytes cultured with FGF2v1 than for chondrocytes cultured without FGF2v1 or for MSCs, but there was no difference between the chondrogenic potential for cultures derived from more or less OA affected areas. All cultures, whether of MSCs or chondrocytes, were positively stained with Alcian blue and safranin O, indicative of the presence of sulfated proteoglycans (Fig. 4). Measurement of the diameter of the pellets from moderate or severely affected OA-derived chondrocytes demonstrated no significant difference between the pellet size (2 vs 2.1 mm, respectively; Table 2). However, smaller pellets were obtained for the BM-MSC (cultured with FGF2v1) and for the chondrocytes cultured without FGF2v1 (1.4, 1.6, or 1.6 mm for BM-MSC; moderate, no ligand; or severe, no ligand, respectively; Table 2).


The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Histology sections of micro mass cultures of mesenchymal stem cells (MSCs) and moderate or severely affected chondrocytes. Micro mass cultures of MSCs (hBM-MSC) or chondrocytes (hAC) derived from moderate or severely affected osteoarthritis tissue from a representative subject were analyzed by histology (hematoxylin and eosin [H&E] stain; bars = 100 µm) with (F2v1) or without (no ligand) the presence of FGF2v1 in the culture medium used to expand the cells. The presence of proteoglycans and sulfated glycosaminoglycans was examined by Alcian blue (AB) and safranin O (SO) stains (bars = 100 µm).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4300788&req=5

fig4-1947603510380899: Histology sections of micro mass cultures of mesenchymal stem cells (MSCs) and moderate or severely affected chondrocytes. Micro mass cultures of MSCs (hBM-MSC) or chondrocytes (hAC) derived from moderate or severely affected osteoarthritis tissue from a representative subject were analyzed by histology (hematoxylin and eosin [H&E] stain; bars = 100 µm) with (F2v1) or without (no ligand) the presence of FGF2v1 in the culture medium used to expand the cells. The presence of proteoglycans and sulfated glycosaminoglycans was examined by Alcian blue (AB) and safranin O (SO) stains (bars = 100 µm).
Mentions: A representative histological analysis of the micro mass cultures is shown in Figure 4. Larger pellets were obtained for chondrocytes cultured with FGF2v1 than for chondrocytes cultured without FGF2v1 or for MSCs, but there was no difference between the chondrogenic potential for cultures derived from more or less OA affected areas. All cultures, whether of MSCs or chondrocytes, were positively stained with Alcian blue and safranin O, indicative of the presence of sulfated proteoglycans (Fig. 4). Measurement of the diameter of the pellets from moderate or severely affected OA-derived chondrocytes demonstrated no significant difference between the pellet size (2 vs 2.1 mm, respectively; Table 2). However, smaller pellets were obtained for the BM-MSC (cultured with FGF2v1) and for the chondrocytes cultured without FGF2v1 (1.4, 1.6, or 1.6 mm for BM-MSC; moderate, no ligand; or severe, no ligand, respectively; Table 2).

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus