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The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus

Histology sections of micro mass cultures of healthy mesenchymal stem cells (MSCs). Micro mass cultures of MSCs from healthy human bone marrow (purchased from Lonza, Allendale, NJ) expanded without or with FGF2v1 and analyzed by histology (hematoxylin and eosin [H&E] stain, Alcian blue [AB], and safranin O [SO] stains). Bars = 100 µm. In the presence of ligand, the pellets are much bigger than without ligand, and there is positive staining for both proteoglycans (Alcian blue) and sulfated glycosaminoglycans (safranin O).
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fig3-1947603510380899: Histology sections of micro mass cultures of healthy mesenchymal stem cells (MSCs). Micro mass cultures of MSCs from healthy human bone marrow (purchased from Lonza, Allendale, NJ) expanded without or with FGF2v1 and analyzed by histology (hematoxylin and eosin [H&E] stain, Alcian blue [AB], and safranin O [SO] stains). Bars = 100 µm. In the presence of ligand, the pellets are much bigger than without ligand, and there is positive staining for both proteoglycans (Alcian blue) and sulfated glycosaminoglycans (safranin O).

Mentions: Analysis of the chondrogenic potential of control MSCs (isolated from a healthy donor) in the optimized micro mass culture, a well-accepted method to assess the chondrogenic potential of cells,22,23 revealed that, in the absence of FGF2v1, there was little or no formation of cartilage-like matrix (Fig. 3). In contrast, when the cells were grown in the presence of the ligand, there was massive production of extracellular matrix, which stained for total and sulfated proteoglycans (Fig. 3). This difference was also confirmed by qPCR analysis where the expression of Col II in the pellets of MSCs grown without FGF2v1 was undetectable (data not shown), but there were high levels of expression of Col II in the presence of ligand. Because of this, all results with MSCs isolated from the osteoarthritic patients relate only to cells grown with FGF2v1.


The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Histology sections of micro mass cultures of healthy mesenchymal stem cells (MSCs). Micro mass cultures of MSCs from healthy human bone marrow (purchased from Lonza, Allendale, NJ) expanded without or with FGF2v1 and analyzed by histology (hematoxylin and eosin [H&E] stain, Alcian blue [AB], and safranin O [SO] stains). Bars = 100 µm. In the presence of ligand, the pellets are much bigger than without ligand, and there is positive staining for both proteoglycans (Alcian blue) and sulfated glycosaminoglycans (safranin O).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4300788&req=5

fig3-1947603510380899: Histology sections of micro mass cultures of healthy mesenchymal stem cells (MSCs). Micro mass cultures of MSCs from healthy human bone marrow (purchased from Lonza, Allendale, NJ) expanded without or with FGF2v1 and analyzed by histology (hematoxylin and eosin [H&E] stain, Alcian blue [AB], and safranin O [SO] stains). Bars = 100 µm. In the presence of ligand, the pellets are much bigger than without ligand, and there is positive staining for both proteoglycans (Alcian blue) and sulfated glycosaminoglycans (safranin O).
Mentions: Analysis of the chondrogenic potential of control MSCs (isolated from a healthy donor) in the optimized micro mass culture, a well-accepted method to assess the chondrogenic potential of cells,22,23 revealed that, in the absence of FGF2v1, there was little or no formation of cartilage-like matrix (Fig. 3). In contrast, when the cells were grown in the presence of the ligand, there was massive production of extracellular matrix, which stained for total and sulfated proteoglycans (Fig. 3). This difference was also confirmed by qPCR analysis where the expression of Col II in the pellets of MSCs grown without FGF2v1 was undetectable (data not shown), but there were high levels of expression of Col II in the presence of ligand. Because of this, all results with MSCs isolated from the osteoarthritic patients relate only to cells grown with FGF2v1.

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus