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The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus

Comparable analysis of viability and the collagen II/collagen I ratio of freshly isolated chondrocytes from moderate or severely affected osteoarthritis (OA) cartilage. Chondrocytes isolated by enzymatic digestion from moderate or severely affected OA cartilage from the same patient were counted using a Vi-Cell XR, and a box-and-whiskers plot of percentage viability is shown in panel a. Panel b shows the ratio of Col II/Col I relative expression (mean and standard deviation) of the fresh cells immediately after isolation analyzed by qPCR. The viability data were analyzed with a 2-tailed paired t test (19 patients). In panel b, the changes in the ratio of Col II/Col I were analyzed with a 1-tailed paired t test (19 patients). Significant P values are shown.
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fig1-1947603510380899: Comparable analysis of viability and the collagen II/collagen I ratio of freshly isolated chondrocytes from moderate or severely affected osteoarthritis (OA) cartilage. Chondrocytes isolated by enzymatic digestion from moderate or severely affected OA cartilage from the same patient were counted using a Vi-Cell XR, and a box-and-whiskers plot of percentage viability is shown in panel a. Panel b shows the ratio of Col II/Col I relative expression (mean and standard deviation) of the fresh cells immediately after isolation analyzed by qPCR. The viability data were analyzed with a 2-tailed paired t test (19 patients). In panel b, the changes in the ratio of Col II/Col I were analyzed with a 1-tailed paired t test (19 patients). Significant P values are shown.

Mentions: Support for the visual method of assessment of OA severity was given by a number of more objective measurements. Following enzymatic digestion, the total density of viable cells was found to be very similar between the moderate or severely affected cartilage (3.5 and 3.14 × 106 cells/g tissue, respectively). In contrast, cell viability was significantly higher for cells isolated from the more moderate OA regions (63%) compared to that of cells retrieved from the more severely affected regions (55%; P = 0.0004; Fig. 1a). Moreover, the calculated differentiation index was significantly higher: 7.8-fold for the moderate compared to the severely OA affected cells in the primary culture (Fig. 1b).


The Chondrogenic Potential of Mesenchymal Cells and Chondrocytes from Osteoarthritic Subjects: A Comparative Analysis.

Agar G, Blumenstein S, Bar-Ziv Y, Kardosh R, Schrift-Tzadok M, Gal-Levy R, Fischler T, Goldschmid R, Yayon A - Cartilage (2011)

Comparable analysis of viability and the collagen II/collagen I ratio of freshly isolated chondrocytes from moderate or severely affected osteoarthritis (OA) cartilage. Chondrocytes isolated by enzymatic digestion from moderate or severely affected OA cartilage from the same patient were counted using a Vi-Cell XR, and a box-and-whiskers plot of percentage viability is shown in panel a. Panel b shows the ratio of Col II/Col I relative expression (mean and standard deviation) of the fresh cells immediately after isolation analyzed by qPCR. The viability data were analyzed with a 2-tailed paired t test (19 patients). In panel b, the changes in the ratio of Col II/Col I were analyzed with a 1-tailed paired t test (19 patients). Significant P values are shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4300788&req=5

fig1-1947603510380899: Comparable analysis of viability and the collagen II/collagen I ratio of freshly isolated chondrocytes from moderate or severely affected osteoarthritis (OA) cartilage. Chondrocytes isolated by enzymatic digestion from moderate or severely affected OA cartilage from the same patient were counted using a Vi-Cell XR, and a box-and-whiskers plot of percentage viability is shown in panel a. Panel b shows the ratio of Col II/Col I relative expression (mean and standard deviation) of the fresh cells immediately after isolation analyzed by qPCR. The viability data were analyzed with a 2-tailed paired t test (19 patients). In panel b, the changes in the ratio of Col II/Col I were analyzed with a 1-tailed paired t test (19 patients). Significant P values are shown.
Mentions: Support for the visual method of assessment of OA severity was given by a number of more objective measurements. Following enzymatic digestion, the total density of viable cells was found to be very similar between the moderate or severely affected cartilage (3.5 and 3.14 × 106 cells/g tissue, respectively). In contrast, cell viability was significantly higher for cells isolated from the more moderate OA regions (63%) compared to that of cells retrieved from the more severely affected regions (55%; P = 0.0004; Fig. 1a). Moreover, the calculated differentiation index was significantly higher: 7.8-fold for the moderate compared to the severely OA affected cells in the primary culture (Fig. 1b).

Bottom Line: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential.Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage.Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

View Article: PubMed Central - PubMed

Affiliation: Asaf HaRofeh Medical Center, Zrifin, Israel.

ABSTRACT

Objective: The multipotential nature of stem or progenitor cells apparently makes them the ideal choice for any cell therapy, but this as yet remains to be proven. This study (30 subjects) was designed to compare the potential to repair articular cartilage of chondrocytes taken from different regions in osteoarthritic cartilage with that of mesenchymal stem cells prepared from bone marrow of the same subject.

Design: Cartilage biopsies, bone marrow, and blood samples were taken from each of 30 individuals with chronic osteoarthritis (aged 62-85 years) undergoing total knee replacement. The chondrogenic potential of chondrocytes isolated from cartilage biopsies taken from different regions of osteoarthritic cartilage was compared with that of mesenchymal cells by quantitative analysis of several chondrocyte specific markers and an ex vivo cartilage differentiation assay.

Results: Cartilage-derived articular chondrocytes are superior to bone marrow-derived cells when compared for their ex vivo chondrogenic potential. Interestingly, there was marked and significant difference in the expression of chondrocytic markers between chondrocytes derived from adjacent, visually distinct regions of the diseased cartilage. When cultured in the presence of a fibroblast growth factor 2 variant, all cell samples from both tissues showed a high degree of chondrogenic potential.

Conclusions: Although bone marrow-derived mesenchymal cells, when supplemented with the appropriate chondrogenic factors, are a suitable source for autologous cartilage implantation, adult chondroprogenitor cells, particularly those from moderately affected regions of the osteoarthritic joints, demonstrate superior chondrogenic potential.

No MeSH data available.


Related in: MedlinePlus