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Microsatellite markers reveal low levels of population sub-structuring of Plasmodium falciparum in southwestern Nigeria.

Oyebola MK, Idowu ET, Nyang H, Olukosi YA, Otubanjo OA, Nwakanma DC, Awolola ST, Amambua-Ngwa A - Malar. J. (2014)

Bottom Line: No matching multilocus haplotypes were found and analysis of multilocus LD showed no significant index of association.Genetic differentiation between populations was low (ΦPT = 0.017).The absence of detectable population structure of P. falciparum in southwestern Nigeria is evident in the lack of significant differentiation between populations separated by about 200 km.

View Article: PubMed Central - PubMed

Affiliation: Medical Research Council, Gambia Unit, Fajara, The Gambia. angwa@mrc.gm.

ABSTRACT

Background: Genetic diversity studies provide evidence of Plasmodium falciparum differentiation that could affect fitness and adaptation to drugs and target antigens for vaccine development. This study describes the genetic structure of P. falciparum populations in urban and rural sites from southwestern Nigeria.

Methodology: Ten neutral microsatellite loci were genotyped in 196 P. falciparum infections from three localities: Aramoko-Ekiti, a rural community; Lekki, an urban location and Badagry, a peri-urban border settlement. Analysis was performed on the genetic diversity, linkage disequilibrium, population structure and inter-population differentiation.

Results: Allelic diversity values were similar across all populations, with mean expected heterozygosity (HE) values between 0.65 and 0.79. No matching multilocus haplotypes were found and analysis of multilocus LD showed no significant index of association. Genetic differentiation between populations was low (ΦPT = 0.017).

Conclusion: The absence of detectable population structure of P. falciparum in southwestern Nigeria is evident in the lack of significant differentiation between populations separated by about 200 km. This implies that a fairly uniform malaria control strategy may be effective over a wide geographic range in this highly endemic region. However, more wide-scale survey across the country will be required to inform malaria control in this large and densely populated endemic region.

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Related in: MedlinePlus

Map of southwestern Nigeria showing the study areas and the geographic distances between them. Red lines indicate the distances between sites.
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Fig1: Map of southwestern Nigeria showing the study areas and the geographic distances between them. Red lines indicate the distances between sites.

Mentions: Participants presenting with symptoms of malaria at three health facilities each representing three localities in southwestern Nigeria: Aramoko-Ekiti (AMK), a rural community in Ekiti State; Lekki (LEK), an urban community and Badagry (BDG), a peri-urban border community in Lagos State (Figure 1), were recruited between November, 2012 and December, 2013. All participants or their guardians gave written informed consent to provide blood samples for the study. The study protocols were reviewed by the Institutional Review Board of the Nigerian Institute of Medical Research, Lagos (with reference number IRB/12/209). Thick and thin blood films prepared on microscope slides were stained with 10% Giemsa (v/v) and examined under the microscope (Olympus CX21, UK). Plasmodium falciparum-positive samples were spotted on 3 mm Whatmann filter paper (Whatmann International Ltd., Maidstone, UK). Genomic DNA was extracted from punched-out disc from each filter paper dried blood spot using the QIAmp DNA blood midi kit (Qiagen, UK) followed by molecular analyses at The Medical Research Council, Gambia Unit.Figure 1


Microsatellite markers reveal low levels of population sub-structuring of Plasmodium falciparum in southwestern Nigeria.

Oyebola MK, Idowu ET, Nyang H, Olukosi YA, Otubanjo OA, Nwakanma DC, Awolola ST, Amambua-Ngwa A - Malar. J. (2014)

Map of southwestern Nigeria showing the study areas and the geographic distances between them. Red lines indicate the distances between sites.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4300683&req=5

Fig1: Map of southwestern Nigeria showing the study areas and the geographic distances between them. Red lines indicate the distances between sites.
Mentions: Participants presenting with symptoms of malaria at three health facilities each representing three localities in southwestern Nigeria: Aramoko-Ekiti (AMK), a rural community in Ekiti State; Lekki (LEK), an urban community and Badagry (BDG), a peri-urban border community in Lagos State (Figure 1), were recruited between November, 2012 and December, 2013. All participants or their guardians gave written informed consent to provide blood samples for the study. The study protocols were reviewed by the Institutional Review Board of the Nigerian Institute of Medical Research, Lagos (with reference number IRB/12/209). Thick and thin blood films prepared on microscope slides were stained with 10% Giemsa (v/v) and examined under the microscope (Olympus CX21, UK). Plasmodium falciparum-positive samples were spotted on 3 mm Whatmann filter paper (Whatmann International Ltd., Maidstone, UK). Genomic DNA was extracted from punched-out disc from each filter paper dried blood spot using the QIAmp DNA blood midi kit (Qiagen, UK) followed by molecular analyses at The Medical Research Council, Gambia Unit.Figure 1

Bottom Line: No matching multilocus haplotypes were found and analysis of multilocus LD showed no significant index of association.Genetic differentiation between populations was low (ΦPT = 0.017).The absence of detectable population structure of P. falciparum in southwestern Nigeria is evident in the lack of significant differentiation between populations separated by about 200 km.

View Article: PubMed Central - PubMed

Affiliation: Medical Research Council, Gambia Unit, Fajara, The Gambia. angwa@mrc.gm.

ABSTRACT

Background: Genetic diversity studies provide evidence of Plasmodium falciparum differentiation that could affect fitness and adaptation to drugs and target antigens for vaccine development. This study describes the genetic structure of P. falciparum populations in urban and rural sites from southwestern Nigeria.

Methodology: Ten neutral microsatellite loci were genotyped in 196 P. falciparum infections from three localities: Aramoko-Ekiti, a rural community; Lekki, an urban location and Badagry, a peri-urban border settlement. Analysis was performed on the genetic diversity, linkage disequilibrium, population structure and inter-population differentiation.

Results: Allelic diversity values were similar across all populations, with mean expected heterozygosity (HE) values between 0.65 and 0.79. No matching multilocus haplotypes were found and analysis of multilocus LD showed no significant index of association. Genetic differentiation between populations was low (ΦPT = 0.017).

Conclusion: The absence of detectable population structure of P. falciparum in southwestern Nigeria is evident in the lack of significant differentiation between populations separated by about 200 km. This implies that a fairly uniform malaria control strategy may be effective over a wide geographic range in this highly endemic region. However, more wide-scale survey across the country will be required to inform malaria control in this large and densely populated endemic region.

Show MeSH
Related in: MedlinePlus