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Fabrication and characterization of biodegradable polymeric films as a corneal stroma substitute.

Salehi S, Fathi M, Javanmard SH, Barneh F, Moshayedi M - Adv Biomed Res (2015)

Bottom Line: The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro.The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid.Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Engineering, Biomaterials Research Group, Isfahan University of Technology, Isfahan, Iran.

ABSTRACT

Background: Biodegradable elastomeric materials such as poly glycerol sebacate (PGS) have gained much current attention in the field of soft tissue engineering. The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro.

Materials and methods: PGS films were prepared by the casting method. We tried to fabricate PGS with different compositions and various properties as being a viable alternative to the corneal stroma in cornea tissue engineering. The chemical properties of the prepared polymer were investigated by means of attenuated total reflectance - Fourier transform infrared spectroscopy (ATR-FTIR) analysis and the in vitro cytotoxicity was investigated by the Alamarblue method.

Results: The functional groups observed in the PGS FTIR spectrums of PGS with various molar ratios were the same. However, the main difference was the time of completing the cross-linking reaction. The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid. Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.

Conclusions: PGS is a potentially good candidate material for corneal tissue engineering because of its lack of in vitro HCE cell toxicity.

No MeSH data available.


Related in: MedlinePlus

Cell viability evaluated by Alamarblue after 24 h of continuous exposure of cells to poly (glycerol sebacate). There was no significant difference between the groups
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Figure 2: Cell viability evaluated by Alamarblue after 24 h of continuous exposure of cells to poly (glycerol sebacate). There was no significant difference between the groups

Mentions: In this study, Alamarblue was used to measure the cytotoxicity of PGS polymer on epithelial corneal cells. Incubating Alamarblue with cells and calculating its percent reduction according to the related formula revealed no significant difference between viability of PGS treated and non treated cells (P ≥ 0.05), indicating that polymer did not pose any toxic effect to the corneal cells Figure 2. Cells in the PGS sample wells were viable and showed normal morphology.


Fabrication and characterization of biodegradable polymeric films as a corneal stroma substitute.

Salehi S, Fathi M, Javanmard SH, Barneh F, Moshayedi M - Adv Biomed Res (2015)

Cell viability evaluated by Alamarblue after 24 h of continuous exposure of cells to poly (glycerol sebacate). There was no significant difference between the groups
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300602&req=5

Figure 2: Cell viability evaluated by Alamarblue after 24 h of continuous exposure of cells to poly (glycerol sebacate). There was no significant difference between the groups
Mentions: In this study, Alamarblue was used to measure the cytotoxicity of PGS polymer on epithelial corneal cells. Incubating Alamarblue with cells and calculating its percent reduction according to the related formula revealed no significant difference between viability of PGS treated and non treated cells (P ≥ 0.05), indicating that polymer did not pose any toxic effect to the corneal cells Figure 2. Cells in the PGS sample wells were viable and showed normal morphology.

Bottom Line: The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro.The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid.Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.

View Article: PubMed Central - PubMed

Affiliation: Department of Materials Engineering, Biomaterials Research Group, Isfahan University of Technology, Isfahan, Iran.

ABSTRACT

Background: Biodegradable elastomeric materials such as poly glycerol sebacate (PGS) have gained much current attention in the field of soft tissue engineering. The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial (HCE) cells viability in vitro.

Materials and methods: PGS films were prepared by the casting method. We tried to fabricate PGS with different compositions and various properties as being a viable alternative to the corneal stroma in cornea tissue engineering. The chemical properties of the prepared polymer were investigated by means of attenuated total reflectance - Fourier transform infrared spectroscopy (ATR-FTIR) analysis and the in vitro cytotoxicity was investigated by the Alamarblue method.

Results: The functional groups observed in the PGS FTIR spectrums of PGS with various molar ratios were the same. However, the main difference was the time of completing the cross-linking reaction. The PGS prepared by 2:3 ratio as a molar ratio had the fastest and the 3:2 ratio had the lowest cross-linking rate because of the higher amount of sebacic acid. Results of the Alamarblue cytotoxicity test assay showed no deleterious effect on HCE cell viability and proliferation.

Conclusions: PGS is a potentially good candidate material for corneal tissue engineering because of its lack of in vitro HCE cell toxicity.

No MeSH data available.


Related in: MedlinePlus