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Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients.

Rastegari A, Rabbani M, Sadeghi HM, Imani EF, Hasanzadeh A, Moazen F - Adv Biomed Res (2015)

Bottom Line: Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate.P < 0.05 was considered.The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCNJ11 (E23K) mutation increases the risk of T2D. Therefore, present study was designed to investigate the association between E23K polymorphism of KCNJ11 gene and type 2 diabetes mellitus (T2DM) in the Iranian population.

Materials and methods: The type of study was case-control and 40 unrelated subjects, including 20 healthy controls and 20 diabetic patients were recruited (diagnosed based on American Diabetes Association criteria). Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure PCR Template Preparation Kit, PCR-restriction fragment length polymorphism method was used to detect KCNJ11 E23K gene polymorphism. BanII restriction enzyme was used for digestion. Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate. P < 0.05 was considered.

Results: We found that the carrier homozygous for KK genotype are susceptible to T2D (0.049) and in patients the frequency of K allele was higher than control subjects (0.048).

Conclusion: The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.

No MeSH data available.


Related in: MedlinePlus

Polymerase chain reaction products digested by BanII restriction enzyme for the detection of E23K polymorphism of KANJ11 gene. Samples were electrophoresed using a 12% polyacrylamide gel and subsequent staining with ethidium bromide. Lane 1: Deoxyribonucleic acid ladder; lane 2: E23K heterozygotes; lane 3: K23 homozygotes; lane 4: E23 homozygotes. Small bands of 32 bp and 28 bp are not visible in this figure
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Figure 2: Polymerase chain reaction products digested by BanII restriction enzyme for the detection of E23K polymorphism of KANJ11 gene. Samples were electrophoresed using a 12% polyacrylamide gel and subsequent staining with ethidium bromide. Lane 1: Deoxyribonucleic acid ladder; lane 2: E23K heterozygotes; lane 3: K23 homozygotes; lane 4: E23 homozygotes. Small bands of 32 bp and 28 bp are not visible in this figure

Mentions: PCR amplification of KCNJ11 gene was performed and produced a 210 bp DNA band [Figure 1]. This product digested and gave different patterns as follows: E: 150 bp, 32 bp and 28 bp; K: 178 bp, 32 bp and EK: 178 bp, 150 bp, 32 bp and 28 bp [Figure 2].


Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients.

Rastegari A, Rabbani M, Sadeghi HM, Imani EF, Hasanzadeh A, Moazen F - Adv Biomed Res (2015)

Polymerase chain reaction products digested by BanII restriction enzyme for the detection of E23K polymorphism of KANJ11 gene. Samples were electrophoresed using a 12% polyacrylamide gel and subsequent staining with ethidium bromide. Lane 1: Deoxyribonucleic acid ladder; lane 2: E23K heterozygotes; lane 3: K23 homozygotes; lane 4: E23 homozygotes. Small bands of 32 bp and 28 bp are not visible in this figure
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300590&req=5

Figure 2: Polymerase chain reaction products digested by BanII restriction enzyme for the detection of E23K polymorphism of KANJ11 gene. Samples were electrophoresed using a 12% polyacrylamide gel and subsequent staining with ethidium bromide. Lane 1: Deoxyribonucleic acid ladder; lane 2: E23K heterozygotes; lane 3: K23 homozygotes; lane 4: E23 homozygotes. Small bands of 32 bp and 28 bp are not visible in this figure
Mentions: PCR amplification of KCNJ11 gene was performed and produced a 210 bp DNA band [Figure 1]. This product digested and gave different patterns as follows: E: 150 bp, 32 bp and 28 bp; K: 178 bp, 32 bp and EK: 178 bp, 150 bp, 32 bp and 28 bp [Figure 2].

Bottom Line: Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate.P < 0.05 was considered.The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCNJ11 (E23K) mutation increases the risk of T2D. Therefore, present study was designed to investigate the association between E23K polymorphism of KCNJ11 gene and type 2 diabetes mellitus (T2DM) in the Iranian population.

Materials and methods: The type of study was case-control and 40 unrelated subjects, including 20 healthy controls and 20 diabetic patients were recruited (diagnosed based on American Diabetes Association criteria). Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure PCR Template Preparation Kit, PCR-restriction fragment length polymorphism method was used to detect KCNJ11 E23K gene polymorphism. BanII restriction enzyme was used for digestion. Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate. P < 0.05 was considered.

Results: We found that the carrier homozygous for KK genotype are susceptible to T2D (0.049) and in patients the frequency of K allele was higher than control subjects (0.048).

Conclusion: The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.

No MeSH data available.


Related in: MedlinePlus