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Molecular basis for the effects of zinc deficiency on spermatogenesis: An experimental study in the Sprague-dawley rat model.

Omu AE, Al-Azemi MK, Al-Maghrebi M, Mathew CT, Omu FE, Kehinde EO, Anim JT, Oriowo MA, Memon A - Indian J Urol (2015 Jan-Mar)

Bottom Line: The objective of this study is to investigate the molecular mechanisms underlying the effects of zinc deficiency on spermatogenesis in the Sprague-Dawley (SD) rat.Zinc deficiency is associated with impaired spermatogenesis because of reduced testosterone production, increased oxidative stress and apoptosis.These findings suggest that zinc has a role in male reproduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Faculty of Medicine, Kuwait University, Kuwait.

ABSTRACT

Introduction: The objective of this study is to investigate the molecular mechanisms underlying the effects of zinc deficiency on spermatogenesis in the Sprague-Dawley (SD) rat.

Materials and methods: Three groups of eight adult male SD rats were maintained for 4 weeks on a normal diet as control, zinc deficient diet and zinc deficient diet with zinc supplementation of 28 mg zinc/kg body weight respectively. Using standard techniques, the following parameters were compared between the three groups of experimental animals at the end of 4 weeks: (a) Serum zinc, magnesium (Mg), copper (Cu), selenium (Se) and cadmium (Cd), (b) serum sex hormones, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPX), (c) interleukin-4 (IL-4), tumor necrosis factor-alpha (TNF-α), Bcl-2, Bax and caspase-3 expression in the testes, (d) assessment of apoptosis of testicular cells using electron microscopy and (e) testicular volume and histology using the orchidometer and Johnsen score, respectively.

Results: The zinc deficient group showed a reduction of testicular volume, serum concentrations of Zn, Cu, Se, Mg, SOD, GPX, IL-4, Bcl-2 and testosterone (P < 0.05), as well as increased levels of serum Cd, MDA and tissue TNF-α, Bax, caspase-3 and apoptosis of the germ cells (P < 0.05) compared with control and zinc supplementation groups.

Conclusion: Zinc deficiency is associated with impaired spermatogenesis because of reduced testosterone production, increased oxidative stress and apoptosis. These findings suggest that zinc has a role in male reproduction.

No MeSH data available.


Related in: MedlinePlus

Electron microscopy of seminiferous tubules in normal rats (a), rats fed zinc deficient diet (b) and rats fed zinc deficient diet with zinc supplementation (c). (×4000) (a) typical pattern of normal round spermatids with peripheral mitochondria (arrows), a characteristic Golgi complex (g) lysosomes arrowheads) and nuclei (n) of maturing spermatids are observed in the seminiferous tubule of normal rat
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Figure 3: Electron microscopy of seminiferous tubules in normal rats (a), rats fed zinc deficient diet (b) and rats fed zinc deficient diet with zinc supplementation (c). (×4000) (a) typical pattern of normal round spermatids with peripheral mitochondria (arrows), a characteristic Golgi complex (g) lysosomes arrowheads) and nuclei (n) of maturing spermatids are observed in the seminiferous tubule of normal rat

Mentions: Electron microscopy evaluation as shown in Figure 3a and b revealed apoptosis of both round and elongated spermatids and maturation arrest at different stages of spermatid development with variation in sizes of the nuclei (n) of maturing spermatids in the seminiferous tubules of rats fed zinc deficient diet [Figure 3b] compared to rats fed normal diet [Figure 3a] or those fed with zinc supplementation [Figure 3c]. Furthermore, the germ cells of the seminiferous tubules of rats fed zinc deficient diet showed degeneration of Golgi apparatus and other cellular organelles [Figure 3b]. Figure 3c shows features comparable to those shown on Figure 3a.


Molecular basis for the effects of zinc deficiency on spermatogenesis: An experimental study in the Sprague-dawley rat model.

Omu AE, Al-Azemi MK, Al-Maghrebi M, Mathew CT, Omu FE, Kehinde EO, Anim JT, Oriowo MA, Memon A - Indian J Urol (2015 Jan-Mar)

Electron microscopy of seminiferous tubules in normal rats (a), rats fed zinc deficient diet (b) and rats fed zinc deficient diet with zinc supplementation (c). (×4000) (a) typical pattern of normal round spermatids with peripheral mitochondria (arrows), a characteristic Golgi complex (g) lysosomes arrowheads) and nuclei (n) of maturing spermatids are observed in the seminiferous tubule of normal rat
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300574&req=5

Figure 3: Electron microscopy of seminiferous tubules in normal rats (a), rats fed zinc deficient diet (b) and rats fed zinc deficient diet with zinc supplementation (c). (×4000) (a) typical pattern of normal round spermatids with peripheral mitochondria (arrows), a characteristic Golgi complex (g) lysosomes arrowheads) and nuclei (n) of maturing spermatids are observed in the seminiferous tubule of normal rat
Mentions: Electron microscopy evaluation as shown in Figure 3a and b revealed apoptosis of both round and elongated spermatids and maturation arrest at different stages of spermatid development with variation in sizes of the nuclei (n) of maturing spermatids in the seminiferous tubules of rats fed zinc deficient diet [Figure 3b] compared to rats fed normal diet [Figure 3a] or those fed with zinc supplementation [Figure 3c]. Furthermore, the germ cells of the seminiferous tubules of rats fed zinc deficient diet showed degeneration of Golgi apparatus and other cellular organelles [Figure 3b]. Figure 3c shows features comparable to those shown on Figure 3a.

Bottom Line: The objective of this study is to investigate the molecular mechanisms underlying the effects of zinc deficiency on spermatogenesis in the Sprague-Dawley (SD) rat.Zinc deficiency is associated with impaired spermatogenesis because of reduced testosterone production, increased oxidative stress and apoptosis.These findings suggest that zinc has a role in male reproduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynaecology, Faculty of Medicine, Kuwait University, Kuwait.

ABSTRACT

Introduction: The objective of this study is to investigate the molecular mechanisms underlying the effects of zinc deficiency on spermatogenesis in the Sprague-Dawley (SD) rat.

Materials and methods: Three groups of eight adult male SD rats were maintained for 4 weeks on a normal diet as control, zinc deficient diet and zinc deficient diet with zinc supplementation of 28 mg zinc/kg body weight respectively. Using standard techniques, the following parameters were compared between the three groups of experimental animals at the end of 4 weeks: (a) Serum zinc, magnesium (Mg), copper (Cu), selenium (Se) and cadmium (Cd), (b) serum sex hormones, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPX), (c) interleukin-4 (IL-4), tumor necrosis factor-alpha (TNF-α), Bcl-2, Bax and caspase-3 expression in the testes, (d) assessment of apoptosis of testicular cells using electron microscopy and (e) testicular volume and histology using the orchidometer and Johnsen score, respectively.

Results: The zinc deficient group showed a reduction of testicular volume, serum concentrations of Zn, Cu, Se, Mg, SOD, GPX, IL-4, Bcl-2 and testosterone (P < 0.05), as well as increased levels of serum Cd, MDA and tissue TNF-α, Bax, caspase-3 and apoptosis of the germ cells (P < 0.05) compared with control and zinc supplementation groups.

Conclusion: Zinc deficiency is associated with impaired spermatogenesis because of reduced testosterone production, increased oxidative stress and apoptosis. These findings suggest that zinc has a role in male reproduction.

No MeSH data available.


Related in: MedlinePlus