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A patchless dissolving microneedle delivery system enabling rapid and efficient transdermal drug delivery.

Lahiji SF, Dangol M, Jung H - Sci Rep (2015)

Bottom Line: Dissolving microneedles (DMNs) are polymeric, microscopic needles that deliver encapsulated drugs in a minimally invasive manner.However, due to wide variations in skin elasticity and the amount of hair on the skin, the arrays fabricated on the patch are often not completely inserted and large amount of loaded materials are not delivered.Here, we report "Microlancer", a novel micropillar based system by which patients can self-administer DMNs and which would also be capable of achieving 97 ± 2% delivery efficiency of the loaded drugs regardless of skin type or the amount of hair on the skin in less than a second.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.

ABSTRACT
Dissolving microneedles (DMNs) are polymeric, microscopic needles that deliver encapsulated drugs in a minimally invasive manner. Currently, DMN arrays are superimposed onto patches that facilitate their insertion into skin. However, due to wide variations in skin elasticity and the amount of hair on the skin, the arrays fabricated on the patch are often not completely inserted and large amount of loaded materials are not delivered. Here, we report "Microlancer", a novel micropillar based system by which patients can self-administer DMNs and which would also be capable of achieving 97 ± 2% delivery efficiency of the loaded drugs regardless of skin type or the amount of hair on the skin in less than a second.

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Comparison of insulin release profiles obtained with the Franz diffusion cell.(a) Comparison of cumulative insulin release profiles of the DMN patch compared with the 50-μm and 100-μm insertions into sections of hairless and (b) hairy pig cadaver skin. (c) Insulin-loaded DMNs on the patch before (above) and 2 h after (below) application onto hairless and (d) hairy pig cadaver skin. (e) A 3 × 3 array of insulin-encapsulated DMNs, fabricated on the Microlancer, before (above) and after (below) application. Scale bars on (c) and (d) are 1 mm. Scale bar for (e) is 2 mm.
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f4: Comparison of insulin release profiles obtained with the Franz diffusion cell.(a) Comparison of cumulative insulin release profiles of the DMN patch compared with the 50-μm and 100-μm insertions into sections of hairless and (b) hairy pig cadaver skin. (c) Insulin-loaded DMNs on the patch before (above) and 2 h after (below) application onto hairless and (d) hairy pig cadaver skin. (e) A 3 × 3 array of insulin-encapsulated DMNs, fabricated on the Microlancer, before (above) and after (below) application. Scale bars on (c) and (d) are 1 mm. Scale bar for (e) is 2 mm.

Mentions: To compare the drug release efficiency of the Microlancer with that of the DMN patch, we set up a Franz diffusion cell for insulin-loaded DMNs. A Franz diffusion cell mimics the natural blood circulation of the body by using a body temperature stimulator receptor and a donor cell (Supplementary Fig. S3). Insulin-loaded (0.2 IU) CMC DMNs were applied to pig cadaver skin; two groups of DMNs were investigated, the DMN patch group and the Microlancer group (n = 3 per group). The sections of pig cadaver skin were then fixed and pressed with a pinch clamp over the Franz diffusion cell. Samples were taken from the receptors at 10, 20, 30, 60 and 120 min, and the amount of insulin in each sample was quantitated using an insulin ELISA kit (n = 3 per group). The insulin release profiles of the Microlancer and the DMN patch differed greatly after only 10 min. While the DMN patch had only released 12 ± 2% of its total insulin content after 10 min, the Microlancer (set at 50 μm) had already delivered 46 ± 1% (p < 0.0001). At 2 h post-application, the DMN patch had released 56 ± 5% of its total insulin content, whereas the Microlancer had released 92 ± 2% (p < 0.0001) (Fig. 4a). To analyze the insertion efficacy of the DMNs into hairy skin, we set up a Franz diffusion cell under the same conditions, except that hairy pig cadaver skin was used. Insulin release profiles were measured at the same time intervals as in the first study. The insulin release profiles of the DMNs inserted by the Microlancer remained constant, whereas the DMNs inserted by the patch had only delivered 26 ± 2% of their total insulin content after 2 h (p = 0.0001) (Fig. 4b). Therefore, these results indicate that, unlike the DMN patch, the Microlancer is capable of inserting DMNs into either hairy or hairless skin with the same level of efficacy.


A patchless dissolving microneedle delivery system enabling rapid and efficient transdermal drug delivery.

Lahiji SF, Dangol M, Jung H - Sci Rep (2015)

Comparison of insulin release profiles obtained with the Franz diffusion cell.(a) Comparison of cumulative insulin release profiles of the DMN patch compared with the 50-μm and 100-μm insertions into sections of hairless and (b) hairy pig cadaver skin. (c) Insulin-loaded DMNs on the patch before (above) and 2 h after (below) application onto hairless and (d) hairy pig cadaver skin. (e) A 3 × 3 array of insulin-encapsulated DMNs, fabricated on the Microlancer, before (above) and after (below) application. Scale bars on (c) and (d) are 1 mm. Scale bar for (e) is 2 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300505&req=5

f4: Comparison of insulin release profiles obtained with the Franz diffusion cell.(a) Comparison of cumulative insulin release profiles of the DMN patch compared with the 50-μm and 100-μm insertions into sections of hairless and (b) hairy pig cadaver skin. (c) Insulin-loaded DMNs on the patch before (above) and 2 h after (below) application onto hairless and (d) hairy pig cadaver skin. (e) A 3 × 3 array of insulin-encapsulated DMNs, fabricated on the Microlancer, before (above) and after (below) application. Scale bars on (c) and (d) are 1 mm. Scale bar for (e) is 2 mm.
Mentions: To compare the drug release efficiency of the Microlancer with that of the DMN patch, we set up a Franz diffusion cell for insulin-loaded DMNs. A Franz diffusion cell mimics the natural blood circulation of the body by using a body temperature stimulator receptor and a donor cell (Supplementary Fig. S3). Insulin-loaded (0.2 IU) CMC DMNs were applied to pig cadaver skin; two groups of DMNs were investigated, the DMN patch group and the Microlancer group (n = 3 per group). The sections of pig cadaver skin were then fixed and pressed with a pinch clamp over the Franz diffusion cell. Samples were taken from the receptors at 10, 20, 30, 60 and 120 min, and the amount of insulin in each sample was quantitated using an insulin ELISA kit (n = 3 per group). The insulin release profiles of the Microlancer and the DMN patch differed greatly after only 10 min. While the DMN patch had only released 12 ± 2% of its total insulin content after 10 min, the Microlancer (set at 50 μm) had already delivered 46 ± 1% (p < 0.0001). At 2 h post-application, the DMN patch had released 56 ± 5% of its total insulin content, whereas the Microlancer had released 92 ± 2% (p < 0.0001) (Fig. 4a). To analyze the insertion efficacy of the DMNs into hairy skin, we set up a Franz diffusion cell under the same conditions, except that hairy pig cadaver skin was used. Insulin release profiles were measured at the same time intervals as in the first study. The insulin release profiles of the DMNs inserted by the Microlancer remained constant, whereas the DMNs inserted by the patch had only delivered 26 ± 2% of their total insulin content after 2 h (p = 0.0001) (Fig. 4b). Therefore, these results indicate that, unlike the DMN patch, the Microlancer is capable of inserting DMNs into either hairy or hairless skin with the same level of efficacy.

Bottom Line: Dissolving microneedles (DMNs) are polymeric, microscopic needles that deliver encapsulated drugs in a minimally invasive manner.However, due to wide variations in skin elasticity and the amount of hair on the skin, the arrays fabricated on the patch are often not completely inserted and large amount of loaded materials are not delivered.Here, we report "Microlancer", a novel micropillar based system by which patients can self-administer DMNs and which would also be capable of achieving 97 ± 2% delivery efficiency of the loaded drugs regardless of skin type or the amount of hair on the skin in less than a second.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Korea.

ABSTRACT
Dissolving microneedles (DMNs) are polymeric, microscopic needles that deliver encapsulated drugs in a minimally invasive manner. Currently, DMN arrays are superimposed onto patches that facilitate their insertion into skin. However, due to wide variations in skin elasticity and the amount of hair on the skin, the arrays fabricated on the patch are often not completely inserted and large amount of loaded materials are not delivered. Here, we report "Microlancer", a novel micropillar based system by which patients can self-administer DMNs and which would also be capable of achieving 97 ± 2% delivery efficiency of the loaded drugs regardless of skin type or the amount of hair on the skin in less than a second.

Show MeSH