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A "green" strategy to construct non-covalent, stable and bioactive coatings on porous MOF nanoparticles.

Agostoni V, Horcajada P, Noiray M, Malanga M, Aykaç A, Jicsinszky L, Vargas-Berenguel A, Semiramoth N, Daoud-Mahammed S, Nicolas V, Martineau C, Taulelle F, Vigneron J, Etcheberry A, Serre C, Gref R - Sci Rep (2015)

Bottom Line: Here we bring the proof of concept that the outer surface of porous nanoMOFs can be specifically functionalized in a rapid, biofriendly and non-covalent manner, leading to stable and versatile coatings.The coating procedure did not affect the nanoMOF porosity, crystallinity, adsorption and release abilities.The stable cyclodextrin-based coating was further functionalized with: i) targeting moieties to increase the nanoMOF interaction with specific receptors and ii) poly(ethylene glycol) chains to escape the immune system.

View Article: PubMed Central - PubMed

Affiliation: Institut Galien, Université Paris-Sud, UMR CNRS 8612, 92290 Chatenay Malabry, France.

ABSTRACT
Nanoparticles made of metal-organic frameworks (nanoMOFs) attract a growing interest in gas storage, separation, catalysis, sensing and more recently, biomedicine. Achieving stable, versatile coatings on highly porous nanoMOFs without altering their ability to adsorb molecules of interest represents today a major challenge. Here we bring the proof of concept that the outer surface of porous nanoMOFs can be specifically functionalized in a rapid, biofriendly and non-covalent manner, leading to stable and versatile coatings. Cyclodextrin molecules bearing strong iron complexing groups (phosphates) were firmly anchored to the nanoMOFs' surface, within only a few minutes, simply by incubation with aqueous nanoMOF suspensions. The coating procedure did not affect the nanoMOF porosity, crystallinity, adsorption and release abilities. The stable cyclodextrin-based coating was further functionalized with: i) targeting moieties to increase the nanoMOF interaction with specific receptors and ii) poly(ethylene glycol) chains to escape the immune system. These results pave the way towards the design of surface-engineered nanoMOFs of interest for applications in the field of targeted drug delivery, catalysis, separation and sensing.

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Related in: MedlinePlus

Interaction between J774 macrophages and CD-P coated MOFs studied by confocal microscopy.Cells were stained in green with calcein, whereas the red signal comes from rhodamin-labelled CD-P-coated nanoMOFs. Images represent three distinct optical sections inside cells at different heights above the glass slide: a) 2.3 μm; b) 4.9 μm and c) 7.6 μm. Cell nucleus appears in black. Bar represents 5 μm.
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f6: Interaction between J774 macrophages and CD-P coated MOFs studied by confocal microscopy.Cells were stained in green with calcein, whereas the red signal comes from rhodamin-labelled CD-P-coated nanoMOFs. Images represent three distinct optical sections inside cells at different heights above the glass slide: a) 2.3 μm; b) 4.9 μm and c) 7.6 μm. Cell nucleus appears in black. Bar represents 5 μm.

Mentions: Noteworthy, CD-P coated MIL-100 nanoMOFs were devoid of toxicity, as the uncoated ones (SI, Fig. S7). No significant toxic effects were detected up to high concentrations regardless the type of cells (IC50 = 280 μg.mL−1 for J774 cell line and >500 μg.mL−1 for MCF7 and LP-1 cell lines; SI, Fig. S7). It was previously shown that the CD-P-R coating was stable in the cell culture media (Fig. S4). Confocal microscopy investigations (Fig. 6, particles appear in red) show MOFs particles having penetrated within J774 macrophages with their rhodaminylated coating.


A "green" strategy to construct non-covalent, stable and bioactive coatings on porous MOF nanoparticles.

Agostoni V, Horcajada P, Noiray M, Malanga M, Aykaç A, Jicsinszky L, Vargas-Berenguel A, Semiramoth N, Daoud-Mahammed S, Nicolas V, Martineau C, Taulelle F, Vigneron J, Etcheberry A, Serre C, Gref R - Sci Rep (2015)

Interaction between J774 macrophages and CD-P coated MOFs studied by confocal microscopy.Cells were stained in green with calcein, whereas the red signal comes from rhodamin-labelled CD-P-coated nanoMOFs. Images represent three distinct optical sections inside cells at different heights above the glass slide: a) 2.3 μm; b) 4.9 μm and c) 7.6 μm. Cell nucleus appears in black. Bar represents 5 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300503&req=5

f6: Interaction between J774 macrophages and CD-P coated MOFs studied by confocal microscopy.Cells were stained in green with calcein, whereas the red signal comes from rhodamin-labelled CD-P-coated nanoMOFs. Images represent three distinct optical sections inside cells at different heights above the glass slide: a) 2.3 μm; b) 4.9 μm and c) 7.6 μm. Cell nucleus appears in black. Bar represents 5 μm.
Mentions: Noteworthy, CD-P coated MIL-100 nanoMOFs were devoid of toxicity, as the uncoated ones (SI, Fig. S7). No significant toxic effects were detected up to high concentrations regardless the type of cells (IC50 = 280 μg.mL−1 for J774 cell line and >500 μg.mL−1 for MCF7 and LP-1 cell lines; SI, Fig. S7). It was previously shown that the CD-P-R coating was stable in the cell culture media (Fig. S4). Confocal microscopy investigations (Fig. 6, particles appear in red) show MOFs particles having penetrated within J774 macrophages with their rhodaminylated coating.

Bottom Line: Here we bring the proof of concept that the outer surface of porous nanoMOFs can be specifically functionalized in a rapid, biofriendly and non-covalent manner, leading to stable and versatile coatings.The coating procedure did not affect the nanoMOF porosity, crystallinity, adsorption and release abilities.The stable cyclodextrin-based coating was further functionalized with: i) targeting moieties to increase the nanoMOF interaction with specific receptors and ii) poly(ethylene glycol) chains to escape the immune system.

View Article: PubMed Central - PubMed

Affiliation: Institut Galien, Université Paris-Sud, UMR CNRS 8612, 92290 Chatenay Malabry, France.

ABSTRACT
Nanoparticles made of metal-organic frameworks (nanoMOFs) attract a growing interest in gas storage, separation, catalysis, sensing and more recently, biomedicine. Achieving stable, versatile coatings on highly porous nanoMOFs without altering their ability to adsorb molecules of interest represents today a major challenge. Here we bring the proof of concept that the outer surface of porous nanoMOFs can be specifically functionalized in a rapid, biofriendly and non-covalent manner, leading to stable and versatile coatings. Cyclodextrin molecules bearing strong iron complexing groups (phosphates) were firmly anchored to the nanoMOFs' surface, within only a few minutes, simply by incubation with aqueous nanoMOF suspensions. The coating procedure did not affect the nanoMOF porosity, crystallinity, adsorption and release abilities. The stable cyclodextrin-based coating was further functionalized with: i) targeting moieties to increase the nanoMOF interaction with specific receptors and ii) poly(ethylene glycol) chains to escape the immune system. These results pave the way towards the design of surface-engineered nanoMOFs of interest for applications in the field of targeted drug delivery, catalysis, separation and sensing.

Show MeSH
Related in: MedlinePlus