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Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

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Related in: MedlinePlus

Effect of chronic intake of 0.2% CIN included in the diet of obese mice on blood glucose after a gavage with glucose.After 5 weeks of consumption of high fat diet containing 0.2% of CIN, an oral glucose tolerance test (OGTT) was performed by gavaging the animal with 2 g/kg bw of 20% D-glucose in water. Glucose was assessed with a glucometer before and 15, 30, 60, and 120 min after glucose administration. Blood glucose levels are significantly lower for CIN treated animals as compared to the controls for each time point before and after glucose gavage. (*, p<0.05; **, p<0.01; n = 12; error bars: SEM).
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f8: Effect of chronic intake of 0.2% CIN included in the diet of obese mice on blood glucose after a gavage with glucose.After 5 weeks of consumption of high fat diet containing 0.2% of CIN, an oral glucose tolerance test (OGTT) was performed by gavaging the animal with 2 g/kg bw of 20% D-glucose in water. Glucose was assessed with a glucometer before and 15, 30, 60, and 120 min after glucose administration. Blood glucose levels are significantly lower for CIN treated animals as compared to the controls for each time point before and after glucose gavage. (*, p<0.05; **, p<0.01; n = 12; error bars: SEM).

Mentions: To test whether the ability to use glucose was changed after the treatment with CIN, an oral glucose tolerance test (OGTT) was performed in fasting conditions during the last week of the chronic treatment. Mice treated with CIN showed statistically significant decrease of blood glucose levels after a glucose load. These mice showed already a reduction of 10.6% of blood glucose levels in fasting conditions compared to the control group. During the OGTT, CIN treated mice showed a reduction of 21%, 29.8%, 25% and 8.25% of blood glucose levels after 15′, 30′, 60′ and 120′ respectively after the ingestion of glucose (Fig. 8). However, plasma insulin levels in the fasting state (Table 1) and during the OGTT (data not shown) were unchanged by the CIN treatment.


Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Effect of chronic intake of 0.2% CIN included in the diet of obese mice on blood glucose after a gavage with glucose.After 5 weeks of consumption of high fat diet containing 0.2% of CIN, an oral glucose tolerance test (OGTT) was performed by gavaging the animal with 2 g/kg bw of 20% D-glucose in water. Glucose was assessed with a glucometer before and 15, 30, 60, and 120 min after glucose administration. Blood glucose levels are significantly lower for CIN treated animals as compared to the controls for each time point before and after glucose gavage. (*, p<0.05; **, p<0.01; n = 12; error bars: SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300502&req=5

f8: Effect of chronic intake of 0.2% CIN included in the diet of obese mice on blood glucose after a gavage with glucose.After 5 weeks of consumption of high fat diet containing 0.2% of CIN, an oral glucose tolerance test (OGTT) was performed by gavaging the animal with 2 g/kg bw of 20% D-glucose in water. Glucose was assessed with a glucometer before and 15, 30, 60, and 120 min after glucose administration. Blood glucose levels are significantly lower for CIN treated animals as compared to the controls for each time point before and after glucose gavage. (*, p<0.05; **, p<0.01; n = 12; error bars: SEM).
Mentions: To test whether the ability to use glucose was changed after the treatment with CIN, an oral glucose tolerance test (OGTT) was performed in fasting conditions during the last week of the chronic treatment. Mice treated with CIN showed statistically significant decrease of blood glucose levels after a glucose load. These mice showed already a reduction of 10.6% of blood glucose levels in fasting conditions compared to the control group. During the OGTT, CIN treated mice showed a reduction of 21%, 29.8%, 25% and 8.25% of blood glucose levels after 15′, 30′, 60′ and 120′ respectively after the ingestion of glucose (Fig. 8). However, plasma insulin levels in the fasting state (Table 1) and during the OGTT (data not shown) were unchanged by the CIN treatment.

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

Show MeSH
Related in: MedlinePlus