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Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

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Effect of CIN gavage on gastric emptying of TRPA1 (-/-) mice.After a overnight fasting, WT and TRPA1 (-/-) animals received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water containing a fluorescent tracer. One hour after the gavage the presence of the dye in the stomach was measured. There was no significant difference in the content of dye in the stomach of TRPA1(-/-) animals gavaged or not with CIN, whereas we observed an increase of the presence of dye in WT animals gavaged with CIN compared to those gavaged with water. (*, p<0.05; n.s., not significant; n = 6; error bars: SEM).
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f6: Effect of CIN gavage on gastric emptying of TRPA1 (-/-) mice.After a overnight fasting, WT and TRPA1 (-/-) animals received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water containing a fluorescent tracer. One hour after the gavage the presence of the dye in the stomach was measured. There was no significant difference in the content of dye in the stomach of TRPA1(-/-) animals gavaged or not with CIN, whereas we observed an increase of the presence of dye in WT animals gavaged with CIN compared to those gavaged with water. (*, p<0.05; n.s., not significant; n = 6; error bars: SEM).

Mentions: Secondly, it was tested if CIN affects gastric emptying and intestinal transit. Mice were gavaged with a non-absorbable fluorogenic marker (FITC-dextran; MW 70,000) with or without 250 mg/kg bw of CIN. No significant differences in the calculation of the geometric center were observed in animals treated with or without CIN: 6.205 ± 0.253 versus 6.325 ± 0.259 (geometric center: mean segment number ± SEM). The gastric emptying rate however was significantly slower in mice gavaged with CIN (Fig. 5B). Indeed, in mice gavaged with CIN, 19.2% of the FITC-dextran remained in the stomach in contrast to 4.6% in mice gavaged with control solution. To verify the role of TRPA1 in the effect of CIN on gastric emptying the same experiment was performed using TRPA1 (-/-) mice9 and the proper control mice. In TRPA1 (-/-) mice the difference in gastric emptying was not significant (Fig. 6).


Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Effect of CIN gavage on gastric emptying of TRPA1 (-/-) mice.After a overnight fasting, WT and TRPA1 (-/-) animals received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water containing a fluorescent tracer. One hour after the gavage the presence of the dye in the stomach was measured. There was no significant difference in the content of dye in the stomach of TRPA1(-/-) animals gavaged or not with CIN, whereas we observed an increase of the presence of dye in WT animals gavaged with CIN compared to those gavaged with water. (*, p<0.05; n.s., not significant; n = 6; error bars: SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300502&req=5

f6: Effect of CIN gavage on gastric emptying of TRPA1 (-/-) mice.After a overnight fasting, WT and TRPA1 (-/-) animals received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water containing a fluorescent tracer. One hour after the gavage the presence of the dye in the stomach was measured. There was no significant difference in the content of dye in the stomach of TRPA1(-/-) animals gavaged or not with CIN, whereas we observed an increase of the presence of dye in WT animals gavaged with CIN compared to those gavaged with water. (*, p<0.05; n.s., not significant; n = 6; error bars: SEM).
Mentions: Secondly, it was tested if CIN affects gastric emptying and intestinal transit. Mice were gavaged with a non-absorbable fluorogenic marker (FITC-dextran; MW 70,000) with or without 250 mg/kg bw of CIN. No significant differences in the calculation of the geometric center were observed in animals treated with or without CIN: 6.205 ± 0.253 versus 6.325 ± 0.259 (geometric center: mean segment number ± SEM). The gastric emptying rate however was significantly slower in mice gavaged with CIN (Fig. 5B). Indeed, in mice gavaged with CIN, 19.2% of the FITC-dextran remained in the stomach in contrast to 4.6% in mice gavaged with control solution. To verify the role of TRPA1 in the effect of CIN on gastric emptying the same experiment was performed using TRPA1 (-/-) mice9 and the proper control mice. In TRPA1 (-/-) mice the difference in gastric emptying was not significant (Fig. 6).

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

Show MeSH
Related in: MedlinePlus