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Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

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Related in: MedlinePlus

Effect on short term food intake and gastric emptying of mice receiving a CIN gavage.Mice, after overnight fasting, received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water for control animals. Subsequently, either food intake (A) or intestinal transit was measured (B). A: CIN reduces significantly cumulative food intake during the 2 hours after the gavage, compared to the control group. (*, p<0.05; n = 25) B: CIN does not affect the intestinal transit time: geometric center being 6.21 ± 0.25 for the CIN group and 6.32 ± 0.26 for the control group. However, gastric emptying is slowed with CIN compared to the control group, in stomach and the two first segments of the intestine there is significantly more fluorescent dye in CIN group, SI: small intestine (*, p<0.05; **, p<0.01*; ***, p<0.005; n = 6; error bars: SEM).
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f5: Effect on short term food intake and gastric emptying of mice receiving a CIN gavage.Mice, after overnight fasting, received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water for control animals. Subsequently, either food intake (A) or intestinal transit was measured (B). A: CIN reduces significantly cumulative food intake during the 2 hours after the gavage, compared to the control group. (*, p<0.05; n = 25) B: CIN does not affect the intestinal transit time: geometric center being 6.21 ± 0.25 for the CIN group and 6.32 ± 0.26 for the control group. However, gastric emptying is slowed with CIN compared to the control group, in stomach and the two first segments of the intestine there is significantly more fluorescent dye in CIN group, SI: small intestine (*, p<0.05; **, p<0.01*; ***, p<0.005; n = 6; error bars: SEM).

Mentions: Since in vivo co-localization of ghrelin-secreting cells and TRPA1 expression was found, and in vitro a decrease of ghrelin secretion in MGN3-1 cells when stimulated with CIN, we tested, first, if TRPA1 activation has any effect on FI after an acute treatment with CIN. To this end, the impact of a single dose of CIN on FI was measured. Mice received a gavage with 250 mg/kg (bw) of CIN and food intake was measured every two hours during the subsequent 24 hours. Strong and significant reduction of cumulative food intake was found 2 hours after the treatment. In addition, we observed that 10 hours after the gavage, mice treated with CIN, compensated the total amount of food ingested reaching the same quantity as control mice (Fig. 5A).


Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Effect on short term food intake and gastric emptying of mice receiving a CIN gavage.Mice, after overnight fasting, received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water for control animals. Subsequently, either food intake (A) or intestinal transit was measured (B). A: CIN reduces significantly cumulative food intake during the 2 hours after the gavage, compared to the control group. (*, p<0.05; n = 25) B: CIN does not affect the intestinal transit time: geometric center being 6.21 ± 0.25 for the CIN group and 6.32 ± 0.26 for the control group. However, gastric emptying is slowed with CIN compared to the control group, in stomach and the two first segments of the intestine there is significantly more fluorescent dye in CIN group, SI: small intestine (*, p<0.05; **, p<0.01*; ***, p<0.005; n = 6; error bars: SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300502&req=5

f5: Effect on short term food intake and gastric emptying of mice receiving a CIN gavage.Mice, after overnight fasting, received a single gavage of CIN (250 mg/kg bw) or an equivalent volume of water for control animals. Subsequently, either food intake (A) or intestinal transit was measured (B). A: CIN reduces significantly cumulative food intake during the 2 hours after the gavage, compared to the control group. (*, p<0.05; n = 25) B: CIN does not affect the intestinal transit time: geometric center being 6.21 ± 0.25 for the CIN group and 6.32 ± 0.26 for the control group. However, gastric emptying is slowed with CIN compared to the control group, in stomach and the two first segments of the intestine there is significantly more fluorescent dye in CIN group, SI: small intestine (*, p<0.05; **, p<0.01*; ***, p<0.005; n = 6; error bars: SEM).
Mentions: Since in vivo co-localization of ghrelin-secreting cells and TRPA1 expression was found, and in vitro a decrease of ghrelin secretion in MGN3-1 cells when stimulated with CIN, we tested, first, if TRPA1 activation has any effect on FI after an acute treatment with CIN. To this end, the impact of a single dose of CIN on FI was measured. Mice received a gavage with 250 mg/kg (bw) of CIN and food intake was measured every two hours during the subsequent 24 hours. Strong and significant reduction of cumulative food intake was found 2 hours after the treatment. In addition, we observed that 10 hours after the gavage, mice treated with CIN, compensated the total amount of food ingested reaching the same quantity as control mice (Fig. 5A).

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

Show MeSH
Related in: MedlinePlus