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Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

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Regulation of ghrelin secretion by CIN in MGN 3-1 cells.Four hours treatment of MGN 3-1 cells with CIN (100 μM) decreases both octanoyl ghrelin (A) and total ghrelin (B) secreted in the medium. This effect is partially recovered by the inhibition of TRPA1 with HC-030031 (25 µM). No effect is observed on the secretion of ghrelin when the inhibitor is applied alone (A and B). (*, p<0.05; **, p<0.01*; ***, p<0.005 n = 3; error bars: SEM).
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f4: Regulation of ghrelin secretion by CIN in MGN 3-1 cells.Four hours treatment of MGN 3-1 cells with CIN (100 μM) decreases both octanoyl ghrelin (A) and total ghrelin (B) secreted in the medium. This effect is partially recovered by the inhibition of TRPA1 with HC-030031 (25 µM). No effect is observed on the secretion of ghrelin when the inhibitor is applied alone (A and B). (*, p<0.05; **, p<0.01*; ***, p<0.005 n = 3; error bars: SEM).

Mentions: Additionally, MGN 3-1 cells were used to study the effect of CIN on ghrelin secretion. Active and total ghrelin secreted in the cell culture medium 4 hours after stimulation with 100 µM of CIN were measured and we observed that octanoyl ghrelin and total ghrelin secreted were significantly lower (44%) than in control conditions. When 25 µM of the TRPA1 inhibitor HC-030031 was added at the same time than CIN to the cells, 12% of the effect of CIN on ghrelin secretion was blocked and no effect was observed with HC-030031 alone (Fig. 4A). Total ghrelin secreted in the media was affected exactly in the same manner than octanoyl ghrelin by CIN and HC-030031 (Fig. 4B). By Western Blot analysis, the reduction of ghrelin after CIN treatment has been confirmed (Supplementary Figure 1B), suggesting with regards to the qRT-PCR results (Fig. 3C) that CIN impacts ghrelin at a post-transcriptional level.


Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Regulation of ghrelin secretion by CIN in MGN 3-1 cells.Four hours treatment of MGN 3-1 cells with CIN (100 μM) decreases both octanoyl ghrelin (A) and total ghrelin (B) secreted in the medium. This effect is partially recovered by the inhibition of TRPA1 with HC-030031 (25 µM). No effect is observed on the secretion of ghrelin when the inhibitor is applied alone (A and B). (*, p<0.05; **, p<0.01*; ***, p<0.005 n = 3; error bars: SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300502&req=5

f4: Regulation of ghrelin secretion by CIN in MGN 3-1 cells.Four hours treatment of MGN 3-1 cells with CIN (100 μM) decreases both octanoyl ghrelin (A) and total ghrelin (B) secreted in the medium. This effect is partially recovered by the inhibition of TRPA1 with HC-030031 (25 µM). No effect is observed on the secretion of ghrelin when the inhibitor is applied alone (A and B). (*, p<0.05; **, p<0.01*; ***, p<0.005 n = 3; error bars: SEM).
Mentions: Additionally, MGN 3-1 cells were used to study the effect of CIN on ghrelin secretion. Active and total ghrelin secreted in the cell culture medium 4 hours after stimulation with 100 µM of CIN were measured and we observed that octanoyl ghrelin and total ghrelin secreted were significantly lower (44%) than in control conditions. When 25 µM of the TRPA1 inhibitor HC-030031 was added at the same time than CIN to the cells, 12% of the effect of CIN on ghrelin secretion was blocked and no effect was observed with HC-030031 alone (Fig. 4A). Total ghrelin secreted in the media was affected exactly in the same manner than octanoyl ghrelin by CIN and HC-030031 (Fig. 4B). By Western Blot analysis, the reduction of ghrelin after CIN treatment has been confirmed (Supplementary Figure 1B), suggesting with regards to the qRT-PCR results (Fig. 3C) that CIN impacts ghrelin at a post-transcriptional level.

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

Show MeSH
Related in: MedlinePlus