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Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

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Regulation of gene expression by CIN in MGN3-1 cells.Cinnamaldehyde (100 μM) increases TRPA1 (A) and InsR (B) expression significantly but does not affect Ghrelin (C) or GOAT (D) expression. (*; p<0.05, n = 4 error bars: SEM).
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f3: Regulation of gene expression by CIN in MGN3-1 cells.Cinnamaldehyde (100 μM) increases TRPA1 (A) and InsR (B) expression significantly but does not affect Ghrelin (C) or GOAT (D) expression. (*; p<0.05, n = 4 error bars: SEM).

Mentions: First, to confirm the expression of TRPA1 in MGN3-1 cells, the relative amount of TRPA1 mRNA was measured by qRT-PCR, which was similar to the one of ghrelinO-acyltransferase (GOAT) responsible for adding an octanoyl group to pro-ghrelin at position Ser-3. Second, to investigate the possible roles of TRPA1 in MGN3-1 cells, the effect of CIN on the expression of several genes (TRPA1, Insulin receptor (InsR), Ghrelin and GOAT) was measured. Addition of 100 µM CIN up-regulated the expression of two genes: TRPA1 and InsR (Fig. 3A and B). Upregulation of InsR at the protein level was also confirmed by Western Blot (Supplementary Fig. 1A). No significant differences were found for Ghrelin and GOAT mRNA (see Fig. 3C and D).


Anti-obesity and anti-hyperglycemic effects of cinnamaldehyde via altered ghrelin secretion and functional impact on food intake and gastric emptying.

Camacho S, Michlig S, de Senarclens-Bezençon C, Meylan J, Meystre J, Pezzoli M, Markram H, le Coutre J - Sci Rep (2015)

Regulation of gene expression by CIN in MGN3-1 cells.Cinnamaldehyde (100 μM) increases TRPA1 (A) and InsR (B) expression significantly but does not affect Ghrelin (C) or GOAT (D) expression. (*; p<0.05, n = 4 error bars: SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300502&req=5

f3: Regulation of gene expression by CIN in MGN3-1 cells.Cinnamaldehyde (100 μM) increases TRPA1 (A) and InsR (B) expression significantly but does not affect Ghrelin (C) or GOAT (D) expression. (*; p<0.05, n = 4 error bars: SEM).
Mentions: First, to confirm the expression of TRPA1 in MGN3-1 cells, the relative amount of TRPA1 mRNA was measured by qRT-PCR, which was similar to the one of ghrelinO-acyltransferase (GOAT) responsible for adding an octanoyl group to pro-ghrelin at position Ser-3. Second, to investigate the possible roles of TRPA1 in MGN3-1 cells, the effect of CIN on the expression of several genes (TRPA1, Insulin receptor (InsR), Ghrelin and GOAT) was measured. Addition of 100 µM CIN up-regulated the expression of two genes: TRPA1 and InsR (Fig. 3A and B). Upregulation of InsR at the protein level was also confirmed by Western Blot (Supplementary Fig. 1A). No significant differences were found for Ghrelin and GOAT mRNA (see Fig. 3C and D).

Bottom Line: Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown.After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates.Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

View Article: PubMed Central - PubMed

Affiliation: 1] Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland [2] Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

ABSTRACT
Cinnamon extract is associated to different health benefits but the active ingredients or pathways are unknown. Cinnamaldehyde (CIN) imparts the characteristic flavor to cinnamon and is known to be the main agonist of transient receptor potential-ankyrin receptor 1 (TRPA1). Here, expression of TRPA1 in epithelial mouse stomach cells is described. After receiving a single-dose of CIN, mice significantly reduce cumulative food intake and gastric emptying rates. Co-localization of TRPA1 and ghrelin in enteroendocrine cells of the duodenum is observed both in vivo and in the MGN3-1 cell line, a ghrelin secreting cell model, where incubation with CIN up-regulates expression of TRPA1 and Insulin receptor genes. Ghrelin secreted in the culture medium was quantified following CIN stimulation and we observe that octanoyl and total ghrelin are significantly lower than in control conditions. Additionally, obese mice fed for five weeks with CIN-containing diet significantly reduce their cumulative body weight gain and improve glucose tolerance without detectable modification of insulin secretion. Finally, in adipose tissue up-regulation of genes related to fatty acid oxidation was observed. Taken together, the results confirm anti-hyperglycemic and anti-obesity effects of CIN opening a new approach to investigate how certain spice derived compounds regulate endogenous ghrelin release for therapeutic intervention.

Show MeSH
Related in: MedlinePlus