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Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast.

Vaga S, Bernardo-Faura M, Cokelaer T, Maiolica A, Barnes CA, Gillet LC, Hegemann B, van Drogen F, Sharifian H, Klipp E, Peter M, Saez-Rodriguez J, Aebersold R - Mol. Syst. Biol. (2014)

Bottom Line: We observed a pheromone-induced down-regulation of Hog1 phosphorylation due to Gpd1, Ste20, Ptp2, Pbs2, and Ptc1.Distinct Ste20 and Pbs2 phosphosites responded differently to the two stimuli, suggesting these proteins as key mediators of the information exchange.Our results show that the integration of the response to different stimuli requires complex interconnections between signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Institute of Molecular Systems Biology ETH Zürich, Zürich, Switzerland.

No MeSH data available.


Related in: MedlinePlus

Data representation and clustering
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fig03: Data representation and clustering

Mentions: To investigate changes in phosphorylation, we represented the P-peps measured dynamics (Fig3A) as a 3D graph (Fig3B) and as a combination of 2D charts (Fig3C) representing sections through the 3D graph. In the 2D representation, each curve follows the dynamic profile of the corresponding P-pep along one of the two stimuli's axis (Stimulus_1). Each curve differs from the other superimposed curves in the same graph by the period of the application of the second stimulus (Stimulus_2).


Phosphoproteomic analyses reveal novel cross-modulation mechanisms between two signaling pathways in yeast.

Vaga S, Bernardo-Faura M, Cokelaer T, Maiolica A, Barnes CA, Gillet LC, Hegemann B, van Drogen F, Sharifian H, Klipp E, Peter M, Saez-Rodriguez J, Aebersold R - Mol. Syst. Biol. (2014)

Data representation and clustering
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300490&req=5

fig03: Data representation and clustering
Mentions: To investigate changes in phosphorylation, we represented the P-peps measured dynamics (Fig3A) as a 3D graph (Fig3B) and as a combination of 2D charts (Fig3C) representing sections through the 3D graph. In the 2D representation, each curve follows the dynamic profile of the corresponding P-pep along one of the two stimuli's axis (Stimulus_1). Each curve differs from the other superimposed curves in the same graph by the period of the application of the second stimulus (Stimulus_2).

Bottom Line: We observed a pheromone-induced down-regulation of Hog1 phosphorylation due to Gpd1, Ste20, Ptp2, Pbs2, and Ptc1.Distinct Ste20 and Pbs2 phosphosites responded differently to the two stimuli, suggesting these proteins as key mediators of the information exchange.Our results show that the integration of the response to different stimuli requires complex interconnections between signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Institute of Molecular Systems Biology ETH Zürich, Zürich, Switzerland.

No MeSH data available.


Related in: MedlinePlus