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The effect of fatal carbon monoxide poisoning on the equilibria between cell membranes and the electrolyte solution.

Petelska AD, Kotyńska J, Figaszewski ZA - J. Membr. Biol. (2014)

Bottom Line: These parameters were used to calculate the theoretical values of surface charge density.The model was validated by comparison of these values to experimental data, which were determined from the electrophoretic mobility measurements of the blood cells.The experimental and theoretical surface charge density values agree at pH 2-8, and at higher pH, the deviation was observed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University of Bialystok, Al. J. Pilsudskiego 11/4, 15-443, Białystok, Poland, aneta@uwb.edu.pl.

ABSTRACT
The effect of fatal carbon monoxide poisoning on equilibria between cell membranes and surrounding ions was described using a theoretical four-equilibria model. The model was developed to obtain parameters characterizing the interactions between solution ions and erythrocyte or thrombocyte membrane surface. The parameters are the total surface concentrations of both acidic and basic groups C A, C B and their association constants with solution ions K AH, K BOH. These parameters were used to calculate the theoretical values of surface charge density. The model was validated by comparison of these values to experimental data, which were determined from the electrophoretic mobility measurements of the blood cells. The experimental and theoretical surface charge density values agree at pH 2-8, and at higher pH, the deviation was observed.

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pH dependence of surface charge density of thrombocytes: filled circle control, unfilled square fatal carbon monoxide poisoning (the experimental values are indicated by points and the theoretical values by the curve)
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Fig2: pH dependence of surface charge density of thrombocytes: filled circle control, unfilled square fatal carbon monoxide poisoning (the experimental values are indicated by points and the theoretical values by the curve)

Mentions: The effect of fatal carbon monoxide poisoning on equilibria between cell membranes and surrounding ions was described. The experimental data of surface charge density were calculated from measured electrophoretic mobility values using Eq. (11). The measurements were performed at several pH values using 0.155 M NaCl as a supporting electrolyte. The theoretical values of surface charge density were determined by applying Eq. (8) to the experimental data. Experimental and theoretical surface charge density values of the erythrocyte and thrombocyte membranes as a function of pH are presented in Figs. 1 and 2, respectively. The experimental data are indicated by points, and the theoretical data are indicated by the curve.Fig. 1


The effect of fatal carbon monoxide poisoning on the equilibria between cell membranes and the electrolyte solution.

Petelska AD, Kotyńska J, Figaszewski ZA - J. Membr. Biol. (2014)

pH dependence of surface charge density of thrombocytes: filled circle control, unfilled square fatal carbon monoxide poisoning (the experimental values are indicated by points and the theoretical values by the curve)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4300427&req=5

Fig2: pH dependence of surface charge density of thrombocytes: filled circle control, unfilled square fatal carbon monoxide poisoning (the experimental values are indicated by points and the theoretical values by the curve)
Mentions: The effect of fatal carbon monoxide poisoning on equilibria between cell membranes and surrounding ions was described. The experimental data of surface charge density were calculated from measured electrophoretic mobility values using Eq. (11). The measurements were performed at several pH values using 0.155 M NaCl as a supporting electrolyte. The theoretical values of surface charge density were determined by applying Eq. (8) to the experimental data. Experimental and theoretical surface charge density values of the erythrocyte and thrombocyte membranes as a function of pH are presented in Figs. 1 and 2, respectively. The experimental data are indicated by points, and the theoretical data are indicated by the curve.Fig. 1

Bottom Line: These parameters were used to calculate the theoretical values of surface charge density.The model was validated by comparison of these values to experimental data, which were determined from the electrophoretic mobility measurements of the blood cells.The experimental and theoretical surface charge density values agree at pH 2-8, and at higher pH, the deviation was observed.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University of Bialystok, Al. J. Pilsudskiego 11/4, 15-443, Białystok, Poland, aneta@uwb.edu.pl.

ABSTRACT
The effect of fatal carbon monoxide poisoning on equilibria between cell membranes and surrounding ions was described using a theoretical four-equilibria model. The model was developed to obtain parameters characterizing the interactions between solution ions and erythrocyte or thrombocyte membrane surface. The parameters are the total surface concentrations of both acidic and basic groups C A, C B and their association constants with solution ions K AH, K BOH. These parameters were used to calculate the theoretical values of surface charge density. The model was validated by comparison of these values to experimental data, which were determined from the electrophoretic mobility measurements of the blood cells. The experimental and theoretical surface charge density values agree at pH 2-8, and at higher pH, the deviation was observed.

Show MeSH
Related in: MedlinePlus