Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoxic Escherichia coli.
Bottom Line: In order to investigate new effective and inexpensive therapeutic approaches, we analyzed nanoparticles synthesized by a green approach using corresponding salt (silver or zinc nitrate) with aqueous extract of Caltropis procera fruit or leaves.Using the expression levels of the outer membrane porin OmpT as an indicator for cAMP levels, our results suggest that zinc nanoparticles inhibit adenylyl cyclase activity.Finally, we demonstrated that a single oral administration of silver nanoparticles to infant mice colonized with V. cholerae or ETEC significantly reduces the colonization rates of the pathogens by 75- or 100-fold, respectively.
Affiliation: University of Graz, Institute of Molecular Biosciences, BioTechMed-Graz, Humboldtstrasse 50, A-8010 Graz, Austria; South Valley University, Faculty of Science, Qena, Egypt.Show MeSH
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Mentions: Finally, we addressed the impact of Ag-NPs-L on the small intestine colonization of V. cholerae or ETEC using the infant mouse model. Therefore, mice were infected with V. cholerae or ETEC and orally treated with an Ag-NPs-L solution 6 h post-infection. The control group received an equal volume of saline buffer, which was also administered orally. The number of V. cholerae CFU recovered from the small bowel at 24 h post-infection are shown in Fig. 8. All control mice were stable colonized with median colonization levels of 3 × 105 for V. cholerae or 5 × 104 CFU for ETEC, respectively. Treatment with Ag-NPs-L resulted in a significant decrease in the colonization levels, with a 50-fold reduction in case of V. cholerae and 200-fold reduction for ETEC compared to the control groups.
Affiliation: University of Graz, Institute of Molecular Biosciences, BioTechMed-Graz, Humboldtstrasse 50, A-8010 Graz, Austria; South Valley University, Faculty of Science, Qena, Egypt.