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Transcriptional regulation of copper metabolism genes in the liver of fetal and neonatal control and iron-deficient rats.

Lenartowicz M, Kennedy C, Hayes H, McArdle HJ - Biometals (2014)

Bottom Line: Atp7b levels, in contrast, decreased from a maximum early in gestation to low levels in the term and post-natal livers.Ceruloplasmin expression appeared to be diametrically opposite to Atp7b.The other two metallochaperones showed the same pattern of expression as Atox1, with a decrease to term, a rise at Day 1, or a rise after birth followed by a brief decrease at about Day 3.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Kraków, Poland.

ABSTRACT
Copper and iron metabolism have been known to interact for many years. We have previously shown, during pregnancy, that copper levels in the maternal liver rise as a consequence of iron deficiency, but that levels in the fetal liver decrease. In this paper, we measure expression of genes involved in copper metabolism in fetal and postnatal liver, to test whether alterations can explain this observation. Additionally, we study the extent to which gene expression changes in the latter stages of pregnancy and in the perinatal period. Ctr1 expression levels dropped to term, rising again thereafter. There was no difference in gene expression between control and iron deficient animals. Atox1 expression remained approximately stable until term, and then there was a rise to a maximum at about Day 8. Atp7a expression levels remained constant, except for a brief drop at term. Atp7b levels, in contrast, decreased from a maximum early in gestation to low levels in the term and post-natal livers. Ceruloplasmin expression appeared to be diametrically opposite to Atp7b. The other two metallochaperones showed the same pattern of expression as Atox1, with a decrease to term, a rise at Day 1, or a rise after birth followed by a brief decrease at about Day 3. None of the genes were significantly affected by iron deficiency, suggesting that changes in expression cannot explain the altered copper levels in the fetal and neonatal liver.

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Ceruloplasmin mRNA level in the liver through developmental stages of the control and Fe deficient fetus and neonates. The mRNA level is shown relative to ubiquitin. Values with different letters differ significantly (p < 0.05). Data are expressed as mean ± SEM
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Fig6: Ceruloplasmin mRNA level in the liver through developmental stages of the control and Fe deficient fetus and neonates. The mRNA level is shown relative to ubiquitin. Values with different letters differ significantly (p < 0.05). Data are expressed as mean ± SEM

Mentions: The ATP7B Cu-ATPase delivers copper to ceruloplasmin, so we determined expression levels of Cp in the fetal and neonatal liver. Before birth, expression increased in the fetal liver, dropped to their lowest levels at Day 3 and thereafter increased (Fig. 6). There was no difference in mRNA levels between control and Fe-deficient animals. Further, expression levels of Cp were not correlated with expression of Atp7b (data not shown).Fig. 6


Transcriptional regulation of copper metabolism genes in the liver of fetal and neonatal control and iron-deficient rats.

Lenartowicz M, Kennedy C, Hayes H, McArdle HJ - Biometals (2014)

Ceruloplasmin mRNA level in the liver through developmental stages of the control and Fe deficient fetus and neonates. The mRNA level is shown relative to ubiquitin. Values with different letters differ significantly (p < 0.05). Data are expressed as mean ± SEM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4300417&req=5

Fig6: Ceruloplasmin mRNA level in the liver through developmental stages of the control and Fe deficient fetus and neonates. The mRNA level is shown relative to ubiquitin. Values with different letters differ significantly (p < 0.05). Data are expressed as mean ± SEM
Mentions: The ATP7B Cu-ATPase delivers copper to ceruloplasmin, so we determined expression levels of Cp in the fetal and neonatal liver. Before birth, expression increased in the fetal liver, dropped to their lowest levels at Day 3 and thereafter increased (Fig. 6). There was no difference in mRNA levels between control and Fe-deficient animals. Further, expression levels of Cp were not correlated with expression of Atp7b (data not shown).Fig. 6

Bottom Line: Atp7b levels, in contrast, decreased from a maximum early in gestation to low levels in the term and post-natal livers.Ceruloplasmin expression appeared to be diametrically opposite to Atp7b.The other two metallochaperones showed the same pattern of expression as Atox1, with a decrease to term, a rise at Day 1, or a rise after birth followed by a brief decrease at about Day 3.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Kraków, Poland.

ABSTRACT
Copper and iron metabolism have been known to interact for many years. We have previously shown, during pregnancy, that copper levels in the maternal liver rise as a consequence of iron deficiency, but that levels in the fetal liver decrease. In this paper, we measure expression of genes involved in copper metabolism in fetal and postnatal liver, to test whether alterations can explain this observation. Additionally, we study the extent to which gene expression changes in the latter stages of pregnancy and in the perinatal period. Ctr1 expression levels dropped to term, rising again thereafter. There was no difference in gene expression between control and iron deficient animals. Atox1 expression remained approximately stable until term, and then there was a rise to a maximum at about Day 8. Atp7a expression levels remained constant, except for a brief drop at term. Atp7b levels, in contrast, decreased from a maximum early in gestation to low levels in the term and post-natal livers. Ceruloplasmin expression appeared to be diametrically opposite to Atp7b. The other two metallochaperones showed the same pattern of expression as Atox1, with a decrease to term, a rise at Day 1, or a rise after birth followed by a brief decrease at about Day 3. None of the genes were significantly affected by iron deficiency, suggesting that changes in expression cannot explain the altered copper levels in the fetal and neonatal liver.

Show MeSH
Related in: MedlinePlus