Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.
Bottom Line: Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduced the abundance of a protein with about twice the mass of CRACR2A.It made a positive contribution to endothelial tube formation.The data suggest that endothelial cells contain a long variant of CRACR2A which is an EF-hand-containing Rab protein that lacks impact on CRAC channels.
Affiliation: School of Medicine, University of Leeds, Leeds LS2 9JT, UK.Show MeSH
Mentions: To determine if CRACR2A-L has functional significance in endothelial cells we performed an assay in which HUVECs form tube, capillary-like, structures on a bed of fibroblasts, mimicking aspects of angiogenesis (Fig. 4A). Depletion of CRACR2A-L did not prevent tube formation but reduced the overall length of tubes and the number of branch points (Fig. 4B and C). The data suggest that CRACR2A-L is a functional protein of endothelial cells.
Affiliation: School of Medicine, University of Leeds, Leeds LS2 9JT, UK.