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Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.

Wilson LA, McKeown L, Tumova S, Li J, Beech DJ - Biochem. Biophys. Res. Commun. (2014)

Bottom Line: Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduced the abundance of a protein with about twice the mass of CRACR2A.It made a positive contribution to endothelial tube formation.The data suggest that endothelial cells contain a long variant of CRACR2A which is an EF-hand-containing Rab protein that lacks impact on CRAC channels.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, University of Leeds, Leeds LS2 9JT, UK.

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CRACR2A-L depletion suppresses endothelial tube formation. (A) Images of HUVECs labelled with anti-CD31 antibody (green) after forming tube-like structures on fibroblasts, which were not labelled. HUVECs were transfected with control siRNA or CRACR2A siRNA. (B) Summary histograms showing the effects of CRACR2A siRNA on tube length and number of branch points (n/N = 3/21). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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f0020: CRACR2A-L depletion suppresses endothelial tube formation. (A) Images of HUVECs labelled with anti-CD31 antibody (green) after forming tube-like structures on fibroblasts, which were not labelled. HUVECs were transfected with control siRNA or CRACR2A siRNA. (B) Summary histograms showing the effects of CRACR2A siRNA on tube length and number of branch points (n/N = 3/21). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Mentions: To determine if CRACR2A-L has functional significance in endothelial cells we performed an assay in which HUVECs form tube, capillary-like, structures on a bed of fibroblasts, mimicking aspects of angiogenesis (Fig. 4A). Depletion of CRACR2A-L did not prevent tube formation but reduced the overall length of tubes and the number of branch points (Fig. 4B and C). The data suggest that CRACR2A-L is a functional protein of endothelial cells.


Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.

Wilson LA, McKeown L, Tumova S, Li J, Beech DJ - Biochem. Biophys. Res. Commun. (2014)

CRACR2A-L depletion suppresses endothelial tube formation. (A) Images of HUVECs labelled with anti-CD31 antibody (green) after forming tube-like structures on fibroblasts, which were not labelled. HUVECs were transfected with control siRNA or CRACR2A siRNA. (B) Summary histograms showing the effects of CRACR2A siRNA on tube length and number of branch points (n/N = 3/21). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4300414&req=5

f0020: CRACR2A-L depletion suppresses endothelial tube formation. (A) Images of HUVECs labelled with anti-CD31 antibody (green) after forming tube-like structures on fibroblasts, which were not labelled. HUVECs were transfected with control siRNA or CRACR2A siRNA. (B) Summary histograms showing the effects of CRACR2A siRNA on tube length and number of branch points (n/N = 3/21). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Mentions: To determine if CRACR2A-L has functional significance in endothelial cells we performed an assay in which HUVECs form tube, capillary-like, structures on a bed of fibroblasts, mimicking aspects of angiogenesis (Fig. 4A). Depletion of CRACR2A-L did not prevent tube formation but reduced the overall length of tubes and the number of branch points (Fig. 4B and C). The data suggest that CRACR2A-L is a functional protein of endothelial cells.

Bottom Line: Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduced the abundance of a protein with about twice the mass of CRACR2A.It made a positive contribution to endothelial tube formation.The data suggest that endothelial cells contain a long variant of CRACR2A which is an EF-hand-containing Rab protein that lacks impact on CRAC channels.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, University of Leeds, Leeds LS2 9JT, UK.

Show MeSH