Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.
Bottom Line: Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduced the abundance of a protein with about twice the mass of CRACR2A.It made a positive contribution to endothelial tube formation.The data suggest that endothelial cells contain a long variant of CRACR2A which is an EF-hand-containing Rab protein that lacks impact on CRAC channels.
Affiliation: School of Medicine, University of Leeds, Leeds LS2 9JT, UK.Show MeSH
Mentions: Analysis of the distinct long C-terminus of CRACR2A-L indicated G boxes and other sequence similarities characteristic of Rab GTPases (Fig. 3). Construction of a dendrogram placed CRACR2A-L as a previously unrecognised member of the large Rab protein family (Fig. S2). It is relatively unusual amongst the Rab proteins in containing EF hands (Fig. 3), Ca2+-binding motifs that have been shown to be functional in CRACR2A .
Affiliation: School of Medicine, University of Leeds, Leeds LS2 9JT, UK.