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Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

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Related in: MedlinePlus

Innervation of spinal YFP-immunoreactive neurons. Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lumbar enlargement. (A) A YFP-immunoreactive axon forms several close appositions (arrows) on the dendrite of the YFP-immunoreactive spinal neuron shown in Fig. 2B. (B) A YFP-immunoreactive varicosity apposes (arrow) the proximal dendrite of a YFP-immunoreactive spinal neuron. (C) The cell body of a YFP-immunoreactive spinal neuron receives a close apposition (arrow) from a YFP-immunoreactive varicosity. (D) The dendrite of a YFP-positive spinal neuron receives a close apposition from a YFP-positive bouton (arrow) as well as from several ChAT-positive boutons (double arrowheads). Micrographs in montages: A, 12; D, 3. Scale bars: 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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f0045: Innervation of spinal YFP-immunoreactive neurons. Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lumbar enlargement. (A) A YFP-immunoreactive axon forms several close appositions (arrows) on the dendrite of the YFP-immunoreactive spinal neuron shown in Fig. 2B. (B) A YFP-immunoreactive varicosity apposes (arrow) the proximal dendrite of a YFP-immunoreactive spinal neuron. (C) The cell body of a YFP-immunoreactive spinal neuron receives a close apposition (arrow) from a YFP-immunoreactive varicosity. (D) The dendrite of a YFP-positive spinal neuron receives a close apposition from a YFP-positive bouton (arrow) as well as from several ChAT-positive boutons (double arrowheads). Micrographs in montages: A, 12; D, 3. Scale bars: 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Mentions: In the LE, YFP-immunoreactive boutons formed close appositions on the somata or proximal dendrites of a subset of the spinal neurons that contained YFP (Fig. 9A–D). To assess the extent of this innervation, we counted YFP-immunoreactive neurons that were and were not apposed by YFP-immunoreactive axons in transverse sections that were stained for YFP and either ChAT or NOS from seven YFP-PPG mice. Of the 195 YFP-expressing spinal neurons examined, 40 (20.5%) received close appositions from YFP-immunoreactive terminals.


Spinally projecting preproglucagon axons preferentially innervate sympathetic preganglionic neurons.

Llewellyn-Smith IJ, Marina N, Manton RN, Reimann F, Gribble FM, Trapp S - Neuroscience (2014)

Innervation of spinal YFP-immunoreactive neurons. Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lumbar enlargement. (A) A YFP-immunoreactive axon forms several close appositions (arrows) on the dendrite of the YFP-immunoreactive spinal neuron shown in Fig. 2B. (B) A YFP-immunoreactive varicosity apposes (arrow) the proximal dendrite of a YFP-immunoreactive spinal neuron. (C) The cell body of a YFP-immunoreactive spinal neuron receives a close apposition (arrow) from a YFP-immunoreactive varicosity. (D) The dendrite of a YFP-positive spinal neuron receives a close apposition from a YFP-positive bouton (arrow) as well as from several ChAT-positive boutons (double arrowheads). Micrographs in montages: A, 12; D, 3. Scale bars: 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
© Copyright Policy - CC BY
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4300405&req=5

f0045: Innervation of spinal YFP-immunoreactive neurons. Two-color immunoperoxidase staining for YFP (black) and ChAT (brown) in transverse sections through the lumbar enlargement. (A) A YFP-immunoreactive axon forms several close appositions (arrows) on the dendrite of the YFP-immunoreactive spinal neuron shown in Fig. 2B. (B) A YFP-immunoreactive varicosity apposes (arrow) the proximal dendrite of a YFP-immunoreactive spinal neuron. (C) The cell body of a YFP-immunoreactive spinal neuron receives a close apposition (arrow) from a YFP-immunoreactive varicosity. (D) The dendrite of a YFP-positive spinal neuron receives a close apposition from a YFP-positive bouton (arrow) as well as from several ChAT-positive boutons (double arrowheads). Micrographs in montages: A, 12; D, 3. Scale bars: 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Mentions: In the LE, YFP-immunoreactive boutons formed close appositions on the somata or proximal dendrites of a subset of the spinal neurons that contained YFP (Fig. 9A–D). To assess the extent of this innervation, we counted YFP-immunoreactive neurons that were and were not apposed by YFP-immunoreactive axons in transverse sections that were stained for YFP and either ChAT or NOS from seven YFP-PPG mice. Of the 195 YFP-expressing spinal neurons examined, 40 (20.5%) received close appositions from YFP-immunoreactive terminals.

Bottom Line: These results show that brainstem PPG neurons innervate spinal autonomic and somatic motor neurons.SPN receive the densest PPG innervation.Brainstem PPG neurons could directly modulate sympathetic outflow through their spinal inputs to SPN or interneurons.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Medicine, Physiology and Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia.

Show MeSH
Related in: MedlinePlus